Isolation and polymerase chain reaction detection of Mycoplasma pneumoniae in malaysian patients with respiratory tract infections

Isolation and polymerase chain reaction detection of Mycoplasma pneumoniae in malaysian patients with respiratory tract infections

Isolation and polymerase chain reaction (PCR) were performed for detection of Mycoplasma pneumoniae from respiratory tract specimens obtained from 200 adult and 200 pediatric patients. M. pneumoniae was isolated from bronchoalveolar lavage fluid of 1(0.5%) adult patient and 4(2.0%) tracheal aspirate...

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Bibliographic Details
Published in:Southeast Asian journal of tropical medicine and public health Vol. 31; no. 4; pp. 688 - 692
Main Authors: ST, Tay, MY, Habsah, SC, Tan, MY, Rohani
Format: Journal Article
Language:English
Published: Bangkok Southeast Asian Ministers of Education Organization, Regional Tropical Medicine and Public Health Network 01-12-2000
Subjects:
Adult
Bacterial diseases
Bacterial diseases of the respiratory system
Base Sequence
Biological and medical sciences
Child
Child, Preschool
DNA Primers
Female
Human bacterial diseases
Humans
Infectious diseases
Malaysia - epidemiology
Male
Medical sciences
Mycoplasma Infections - epidemiology
Mycoplasma Infections - microbiology
Mycoplasma pneumoniae - genetics
Mycoplasma pneumoniae - isolation & purification
Polymerase Chain Reaction
Respiratory Tract Infections - epidemiology
Respiratory Tract Infections - microbiology
Tropical medicine
Asia
Malaysia
Human
Respiratory disease
Mycoplasma pneumoniae
Bacteriological investigation
Mycoplasmataceae
Infection
Mollicutes
Polymerase chain reaction
Respiratory tract
Mycoplasmatales
Bacteriosis
Bacteria
Detection
Child
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Summary:Isolation and polymerase chain reaction (PCR) were performed for detection of Mycoplasma pneumoniae from respiratory tract specimens obtained from 200 adult and 200 pediatric patients. M. pneumoniae was isolated from bronchoalveolar lavage fluid of 1(0.5%) adult patient and 4(2.0%) tracheal aspirates of pediatric patients. PCR was positive for only one (0.5%) broncoalveolar lavage fluid of an adult patient and fifteen (7.5%) tracheal aspirates of pediatric patients. This study suggested that M. pneumoniae was more frequently detected in pediatric patients and PCR appears to have advantages over isolation, in terms of rapidity and sensitivity.
Bibliography:ObjectType-Article-1
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ISSN:0125-1562