Search Results - "MOYLE, W. R"

Conversion of Human Choriogonadotropin into a Follitropin by Protein Engineering by Campbell, Robert K., Dean-Emig, Diana M., Moyle, William R.

“…Human reproduction is dependent upon the actions of follicle-stimulating hormone (hFSH), luteinizing hormone (hLH), and chorionic gonadotropin (hCG). While the…”
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Desensitization and resensitization of lutropin receptors expressed in transfected Y-1 adrenal cells by Ulaner, GA, Chuang, J, Lin, W, Woodbury, D, Myers, RV, Moyle, WR

“…Stimulation of gonadal cells by lutropins such as human chorionic gonadotropin (hCG) is often transient and followed by down-regulation and/or desensitization…”
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Chimeric proteins can exceed the sum of their parts : Implications for evolution and protein design by CAMPBELL, R. K, BERGERT, E. R, WANG, Y, MORRIS, J. C, MOYLE, W. R

“…Chimeric analogs derived from pairs of homologous proteins routinely exhibit activities found in one or both parents. We describe chimeras of two glycoprotein…”
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Localization of residues that confer antibody binding specificity using human chorionic gonadotropin/luteinizing hormone beta subunit chimeras and mutants by MOYLE, W. R, MATZUK, M. M, CAMPBELL, R. K, COGLIANI, E, DEAN-EMIG, D. M, KRICHEVSKY, A, BARNETT, R. W, BOIME, I

“…The glycoprotein hormones are a family of conserved heterodimeric proteins which share a common alpha subunit but differ in their hormone-specific beta…”
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Leutropin/beta-adrenergic receptor chimeras bind choriogonadotropin and adrenergic ligands but are not expressed at the cell surface by MOYLE, W. R, BERNARD, M. P, MYERS, R. V, MARKO, O. M, STRADER, C. D

“…In some G-protein-coupled receptors (e.g. beta-adrenergic receptor (beta 2 AR)), the ligand-binding pocket is contained within the hydrophobic transmembrane…”
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Co-evolution of ligand-receptor pairs by Moyle, William R, Campbell, Robert K, Myers, Rebecca V, Bernard, Michael P, Han, Yi, Wang, Xiaoying

“…Specific receptors for lutropin (luteinizing hormone; LH) and follitropin (follicle-stimulating hormone; FSH) mediate the actions of human chorionic…”
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Use of Monoclonal Antibodies to Subunits of Human Chorionic Gonadotropin to Examine the Orientation of the Hormone in Its Complex with Receptor by Moyle, W. R., Ehrlich, P. H., Canfield, R. E.

“…Monoclonal antibodies were prepared against the α and β subunits of human chorionic gonadotropin (hCG). Although all were selected on the basis of their…”
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Cloning of rat lutropin (LH) receptor analogs lacking the soybean lectin domain by Bernard, M P, Myers, R V, Moyle, W R

“…cDNAs coding for rat ovarian luteinizing hormone receptor analogs lacking three of the leucine repeats were detected in a library which had been prepared from…”
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Deglycosylation of a bifunctional lutropin-follitropin agonist reduced its follitropin activity more than its lutropin activity by Trout, S W, Han, Y, Myers, R V, Bernard, M P, Moyle, W R

“…To design a drug that blocks the gonadal actions of lutropins and follitropins. Controlled in vitro study. Academic laboratory. None. We removed three…”
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Detection of conformational changes in human chorionic gonadotropin upon binding to rat gonadal receptors by Moyle, W R, Pressey, A, Dean-Emig, D, Anderson, D M, Demeter, M, Lustbader, J, Ehrlich, P

“…After binding to rat testicular or ovarian luteinizing hormone (LH) receptors, human chorionic gonadotropin (hCG) and mammalian LH can be detected with…”
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Role of carbohydrate of human chorionic gonadotropin in the mechanism of hormone action by Moyle, W R, Bahl, O P, März, L

“…The role of the carbohydrate part of human chorionic gonadotropin (hCG) was investigated by measuring the ability of hCG derivatives lacking various sugar…”
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Mixing two monoclonal antibodies yields enhanced affinity for antigen by Ehrlich, PH, Moyle, WR, Moustafa, ZA, Canfield, RE

“…We observed that mixing monoclonal antibodies directed against various epitopes of human chorionic gonadotropin (hCG) can increase the sensitivity of…”
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A circular antibody-antigen complex is responsible for increased affinity shown by mixtures of monoclonal antibodies to human chorionic gonadotropin by Moyle, WR, Anderson, DM, Ehrlich, PH

“…Mixtures of some pairs of monoclonal antibodies that have separate epitopes on the beta-subunit of hCG have increased affinity for the hormone relative to that…”
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Effect of LH on steroidogenesis and cyclic AMP accumulation in rat Leydig cell preparations and mouse tumor Leydig cells by Moyle, W R, Ramachandran, J

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Further characterization of cooperative interactions of monoclonal antibodies by Ehrlich, PH, Moyle, WR, Moustafa, ZA

“…We reported previously that mixtures of some monoclonal antibodies directed against the glycoprotein hormone human chorionic gonadotropin (hCG) had a higher…”
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Model of Human Chorionic Gonadotropin and Lutropin Receptor Interaction That Explains Signal Transduction of the Glycoprotein Hormones (∗) by Moyle, William R., Campbell, Robert K., Rao, S.N. Venkateswara, Ayad, Nagi G., Bernard, Michael P., Han, Yi, Wang, Yanhong

“…The goal of these studies was to devise a model that explains how human chorionic gonadotropin (hCG) interacts with lutropin (LH) receptors to elicit a hormone…”
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Tight attachment of chitin-binding-domain-tagged proteins to surfaces coated with acetylated chitosan by Bernard, Michael P, Cao, Donghui, Myers, Rebecca V, Moyle, William R

“…Several excellent procedures for trapping tagged proteins have been devised, but many of these are expensive, cannot be used outside a limited pH range, fail…”
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Alternatively folded choriogonadotropin analogs. Implications for hormone folding and biological activity by Xing, Y, Lin, W, Jiang, M, Myers, R V, Cao, D, Bernard, M P, Moyle, W R

“…Most heterodimeric proteins are stabilized by intersubunit contacts or disulfide bonds. In contrast, human chorionic gonadotropin (hCG) and other glycoprotein…”
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Potential of a quantal response as a mechanism for oscillatory behavior: implications for our concepts of hormonal control mechanisms by Moyle, W R, Kuczek, T, Bailey, C A

“…This article describes the potential of a quantal (i.e., all-or-none) response as a model for understanding the interactions between endocrine, paracrine and…”
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Threading of a glycosylated protein loop through a protein hole: Implications for combination of human chorionic gonadotropin subunits by Xing, Yongna, Williams, Christopher, Campbell, Robert K., Cook, Stephanie, Knoppers, Maria, Addona, Theresa, Altarocca, Valter, Moyle, William R.

“…Chorionic gonadotropin (hCG) is a heterodimeric placental glycoprotein hormone essential for human reproduction. Twenty hCG β‐subunit residues, termed the…”
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