Search Results - "MOXLEY, Robert A"
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Purification and Identification of Positive Regulators Binding to a Novel Element in the c-Jun Promoter
Published in Biochemistry (Easton) (02-09-2008)“…A putative response element, GAGCCTC, was observed years ago in footprinting analysis of the c-jun promoter, and here we investigate its function in regulating…”
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Journal Article -
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Promoter trapping of c-jun promoter-binding transcription factors
Published in Journal of Chromatography A (10-11-2006)“…A new method called promoter trapping was developed to purify promoter-protein complex using the c-jun promoter (−200 + 81) as a model, which was shown to have…”
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Journal Article -
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Oligonucleotide trapping method for transcription factor purification systematic optimization using electrophoretic mobility shift assay
Published in Journal of Chromatography A (08-04-2005)“…Oligonucleotide trapping, where a transcription factor–DNA response element complex is formed in solution and then recovered (trapped) on a column, was…”
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Journal Article -
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Effect of the detergent Tween-20 on the DNA affinity chromatography of Gal4, C/EBPα, and lac repressor with observations on column regeneration
Published in Journal of Chromatography A (2004)“…C/EBPα, Gal4, and lac repressor, representing three different transcription factor homology families, were expressed as fusion proteins and used to…”
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Journal Article -
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Methods for transcription factor separation
Published in Journal of Chromatography B (25-11-2003)“…Recent advances in the separation of transcription factors (TFs) are reviewed in this article. An overview of the transcription factor families and their…”
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Book Review Journal Article -
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Effect of the detergent Tween-20 on the DNA affinity chromatography of Gal4, C/EBPalpha, and lac repressor with observations on column regeneration
Published in Journal of chromatography. A (23-01-2004)“…C/EBPalpha, Gal4, and lac repressor, representing three different transcription factor homology families, were expressed as fusion proteins and used to…”
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Journal Article -
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Chapter 43 - DNA Affinity Chromatography of Transcription Factors: The Oligonucleotide Trapping Approach
Published in Cell Biology (2006)“…This chapter describes the current oligonucleotide trapping method practiced in laboratories. A column is prepared with the single-stranded oligonucleotide…”
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Book Chapter