Search Results - "MOULIS, Claire"
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Computer-aided engineering of a branching sucrase for the glucodiversification of a tetrasaccharide precursor of S. flexneri antigenic oligosaccharides
Published in Scientific reports (13-10-2021)“…Enzyme engineering approaches have allowed to extend the collection of enzymatic tools available for synthetic purposes. However, controlling the…”
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Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens
Published in Scientific reports (28-01-2021)“…The (chemo-)enzymatic synthesis of oligosaccharides has been hampered by the lack of appropriate enzymatic tools with requisite regio- and…”
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Deciphering an Undecided Enzyme: Investigations of the Structural Determinants Involved in the Linkage Specificity of Alternansucrase
Published in ACS catalysis (01-03-2019)“…Understanding how polymerases catalyze the synthesis of biopolymers is a timely and important issue in generating controlled structures with well-defined…”
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Bacterial α-Glucan and Branching Sucrases from GH70 Family: Discovery, Structure–Function Relationship Studies and Engineering
Published in Microorganisms (Basel) (01-08-2021)“…Glucansucrases and branching sucrases are classified in the family 70 of glycoside hydrolases. They are produced by lactic acid bacteria occupying very diverse…”
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Exopolysaccharide (EPS) synthesis by Oenococcus oeni: from genes to phenotypes
Published in PloS one (05-06-2014)“…Oenococcus oeni is the bacterial species which drives malolactic fermentation in wine. The analysis of 50 genomic sequences of O. oeni (14 already available…”
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Understanding the Polymerization Mechanism of Glycoside-Hydrolase Family 70 Glucansucrases
Published in The Journal of biological chemistry (20-10-2006)“…Glucan formation catalyzed by two GH-family 70 enzymes, Leuconostoc mesenteroides NRRL B-512F dextransucrase and L. mesenteroides NRRL B-1355 alternansucrase,…”
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Computer-Aided Engineering of a Transglycosylase for the Glucosylation of an Unnatural Disaccharide of Relevance for Bacterial Antigen Synthesis
Published in ACS catalysis (06-02-2015)“…The exploration of chemo-enzymatic routes to complex carbohydrates has been hampered by the lack of appropriate enzymatic tools having the substrate…”
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Combinatorial engineering of dextransucrase specificity
Published in PloS one (18-10-2013)“…We used combinatorial engineering to investigate the relationships between structure and linkage specificity of the dextransucrase DSR-S from Leuconostoc…”
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Genome Sequence of Weissella confusa LBAE C39-2, Isolated from a Wheat Sourdough
Published in Journal of Bacteriology (01-03-2012)“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Genome Sequences of Three Leuconostoc citreum Strains, LBAE C10, LBAE C11, and LBAE E16, Isolated from Wheat Sourdoughs
Published in Journal of Bacteriology (01-03-2012)“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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A New Raw-Starch-Digesting α-Amylase: Production Under Solid-State Fermentation on Crude Millet and Biochemical Characterization
Published in Journal of microbiology and biotechnology (01-04-2013)“…A new Bacillus strain degrading starch, named Bacillus sp. UEB-S, was isolated from a southern Tunisian area. Amylase production using solid-state fermentation…”
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Phosphorylated polysaccharides: Applications, natural abundance, and new-to-nature structures generated by chemical and enzymatic functionalisation
Published in Biotechnology advances (01-07-2023)“…Polysaccharides are foreseen as serious candidates for the future generation of polymers, as they are biosourced and biodegradable materials. Their…”
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Regioselective thexyldimethylsilylation of (1➔3)-glucans – Does the linkage type matter
Published in Carbohydrate polymers (01-11-2024)“…α- and β-(1➔3)-linked polysaccharides dissolved in N,N-dimethyl acetamide (DMA) were subjected to conversion with thexyldimethylchlorosilane (TDMS-Cl) in…”
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A New Raw-Starch-Digesting ${\alpha}$-Amylase: Production Under Solid-State Fermentation on Crude Millet and Biochemical Characterization
Published in Journal of microbiology and biotechnology (2013)“…A new Bacillus strain degrading starch, named Bacillus sp. UEB-S, was isolated from a southern Tunisian area. Amylase production using solid-state fermentation…”
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15
Natural and engineered transglycosylases: Green tools for the enzyme-based synthesis of glycoproducts
Published in Current opinion in chemical biology (01-04-2021)“…An increasing number of transglycosylase-based processes provide access to oligosaccharides or glycoconjugates, some of them reaching performance levels…”
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The stability of an mRNA is influenced by its concentration: a potential physical mechanism to regulate gene expression
Published in Nucleic acids research (16-11-2017)“…Changing mRNA stability is a major post-transcriptional way of controlling gene expression, particularly in newly encountered conditions. As the concentration…”
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GH13 amylosucrases and GH70 branching sucrases, atypical enzymes in their respective families
Published in Cellular and molecular life sciences : CMLS (01-07-2016)“…Amylosucrases and branching sucrases are α-retaining transglucosylases found in the glycoside-hydrolase families 13 and 70, respectively, of the clan GH-H…”
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A chemo-enzymatic pathway to expand cellooligosaccharide chemical space through amine bond introduction
Published in Carbohydrate polymers (15-08-2024)“…Enzymatic functionalization of oligosaccharides is a useful and environmentally friendly way to expand their structural chemical space and access to a wider…”
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Processivity of dextransucrases synthesizing very-high-molar-mass dextran is mediated by sugar-binding pockets in domain V
Published in The Journal of biological chemistry (24-04-2020)“…The dextransucrase DSR-OK from the Gram-positive bacterium Oenococcus kitaharae DSM17330 produces a dextran of the highest molar mass reported to date (∼109…”
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Expanding the Enzymatic Polymerization Landscape by Lipid Mesophase Soft Nanoconfinement
Published in Angewandte Chemie International Edition (02-01-2024)“…Soft nanoconfinement can increase chemical reactivity in nature and has therefore led to considerable interest in transferring this universal feature to…”
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