Mapping light strand transcripts near the origin of replication of Xenopus laevis mitochondrial DNA

Mapping light strand transcripts near the origin of replication of Xenopus laevis mitochondrial DNA

Transcription of the light strand of Xenopus laevis mitochondrial DNA initiates at two promoters located approximately 350 to 450 nucleotides upstream from the 5' ends of major D-loop DNA strands. Small RNAs within this region have been mapped by blot hybridization, primer extension and S1 nucl...

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Bibliographic Details
Published in:Nucleic acids research Vol. 18; no. 21; pp. 6377 - 6383
Main Authors: BOGENHAGEN, D. F, MORVILLO, M. V
Format: Journal Article
Language:English
Published: Oxford Oxford University Press 11-11-1990
Subjects:
Animals
Base Sequence
Biological and medical sciences
Chromosome Mapping
Cloning, Molecular
DNA Replication
DNA, Mitochondrial - genetics
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nucleic Acid Hybridization
Oligonucleotide Probes
Replication
RNA - genetics
RNA - isolation & purification
Transcription, Genetic
Xenopus laevis
Xenopus laevis - genetics
Genetic mapping
Vertebrata
Conserved sequence
Molecular processing
RNA
Transcription
Xenopus laevis
Amphibia
Replication
Salientia
Mitochondrial DNA
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Summary:Transcription of the light strand of Xenopus laevis mitochondrial DNA initiates at two promoters located approximately 350 to 450 nucleotides upstream from the 5' ends of major D-loop DNA strands. Small RNAs within this region have been mapped by blot hybridization, primer extension and S1 nuclease protection methods. The results reveal that the large majority of RNAs within this region have 3' termini located at a sequence element, designated CSB 2, that is conserved in sequence and position in Xenopus, mouse, rat and human mtDNA. However, the X. laevis CSB 2 appears to be a site of RNA processing only, since RNA-to-DNA transitions are not detectable at this site. RNAs containing sequences downstream of CSB 2 are extremely rare. A significant fraction of these RNAs are processed by cleavage at a site just upstream of the most predominant 5' ends of D-loop DNAs. We suggest that RNA processing at this site may play a role in priming mtDNA replication.
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ISSN:0305-1048
1362-4962
DOI:10.1093/nar/18.21.6377