Search Results - "MIYAZAKI, CHIE"
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Catalytic Activity in Organic Solvents and Stability of Immobilized Enzymes Depend on the Pore Size and Surface Characteristics of Mesoporous Silica
Published in Chemistry of materials (01-11-2000)“…An enzyme, horseradish peroxidase (HRP), was adsorbed in the manner of the single immersion method on the silica mesoporous materials FSM-16, MCM-41, and…”
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Thermophilic fungal protein disulfide isomerase
Published in Methods in enzymology (1998)Get more information
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Improvement of H2O2 stability of manganese peroxidase by combinatorial mutagenesis and high‐throughput screening using in vitro expression with protein disulfide isomerase
Published in Protein engineering (01-06-2003)“…A functional expression system for a heme protein of Phanerochaete chrysosporium, manganese peroxidase (MnP), was developed using the Escherichia coli in vitro…”
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Immobilized enzymes in ordered mesoporous silica materials and improvement of their stability and catalytic activity in an organic solvent
Published in Microporous and mesoporous materials (01-06-2001)“…Enzyme immobilization in mesoporous materials with various pore sizes was studied. The size of the mesopores was controlled by means of the combination of the…”
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Isolation of a protease-deficient mutant of Bacillus brevis and efficient secretion of a fungal protein disulfide isomerase by the mutant
Published in Journal of bioscience and bioengineering (1999)“…The efficient production of a thermostable protein disulfide isomerase (PDI) was successfully achieved using the newly isolated protease-deficient mutant,…”
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Engineering of the H2O2‐binding pocket region of a recombinant manganese peroxidase to be resistant to H2O2
Published in FEBS letters (30-11-2001)“…The manganese peroxidase produced by Phanerochaete chrysosporium, which catalyzes the oxidation of Mn2+ to Mn3+, is easily inactivated by the hydrogen peroxide…”
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Engineering of the H 2 O 2 ‐binding pocket region of a recombinant manganese peroxidase to be resistant to H 2 O 2
Published in FEBS letters (30-11-2001)“…The manganese peroxidase produced by Phanerochaete chrysosporium , which catalyzes the oxidation of Mn 2+ to Mn 3+ , is easily inactivated by the hydrogen…”
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Engineering of the H 2O 2-binding pocket region of a recombinant manganese peroxidase to be resistant to H 2O 2
Published in FEBS letters (2001)“…The manganese peroxidase produced by Phanerochaete chrysosporium, which catalyzes the oxidation of Mn 2+ to Mn 3+, is easily inactivated by the hydrogen…”
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New strategies for enzyme stabilization involving molecular evolution and immobilization in mesoporous materials
Published in E-journal of surface science and nanotechnology (01-01-2005)“…Heme containing peroxidase, for example manganese peroxidase (MnP), is easily inactivated by the hydrogen peroxide (H2O2) presented in the reaction. Here we…”
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Effects of Placental Extract on Healthy Women’s Body Constitution in Traditional Chinese Medicine: an Open-label Clinical Trial
Published in Japanese Journal of Complementary and Alternative Medicine (14-12-2020)“…We conducted a clinical trial in 26 healthy adult women to evaluate the effect of foods containing placental extract ingested for 12 consecutive weeks on 9…”
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Two-step strategy for alteration of immunoglobulin specificity by in vitro mutagenesis
Published in Methods in molecular biology (Clifton, N.J.) (2002)Get more information
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Changes in the specificity of antibodies by site-specific mutagenesis followed by random mutagenesis
Published in Protein engineering (01-05-1999)“…The specificity for 11-deoxycortisol (11-DOC) of a monoclonal antibody (mAb), designated SCET, was changed to specificity for cortisol (CS) by site-specific…”
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CLINICAL EVALUATION OF A NEW MUSCLE RELAXANT PANCURONIUM BROMIDE
Published in Kurume medical journal (1974)“…Our findings deserve the following conclusions.1) With an initial dose of 0.1 to 0.15 mg/kg, sufficient and excellent muscular relaxation for endotracheal…”
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