Search Results - "MILES, E. W"

Domain Architecture of the Heme-Independent Yeast Cystathionine β-Synthase Provides Insights into Mechanisms of Catalysis and Regulation by Jhee, Kwang-Hwan, McPhie, Peter, Miles, Edith Wilson

“…Cystathionine β-synthase from yeast (Saccharomyces cerevisiae) provides a model system for understanding some of the effects of disease-causing mutations in…”
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Three-dimensional structure of the tryptophan synthase alpha 2 beta 2 multienzyme complex from Salmonella typhimurium by Hyde, C C, Ahmed, S A, Padlan, E A, Miles, E W, Davies, D R

“…The three-dimensional structure of the alpha 2 beta 2 complex of tryptophan synthase from Salmonella typhimurium has been determined by x-ray crystallography…”
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Serine modulates substrate channeling in tryptophan synthase. A novel intersubunit triggering mechanism by ANDERSON, K. S, MILES, E. W, JOHNSON, K. A

“…Tryptophan synthase, an alpha 2 beta 2 complex, is a classic example of an enzyme that is thought to "channel" a metabolic intermediate (indole) from the…”
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Experimental Validation of Cryobot Thermal Models for the Exploration of Ocean Worlds by do Vale Pereira, Paula, Durka, Michael J., Hogan, Bartholomew P., Richmond, Kristof, Smith, Miles W. E., Winebrenner, Dale P., Elam, W. Timothy, Hockman, Benjamin J., Lopez, Alberto, Tanner, Neal, Moor, Joshua, Ralston, James, Alexander, Miriam, Zimmerman, Wayne, Flannery, Nolan, Kuhl, William, Wielgosz, Sarah, Cahoy, Kerri L., Cwik, Thomas A., Stone, William C.

“…Accessing the potentially habitable subsurface waters of Ocean Worlds requires a robotic ice probe (or “cryobot”) to traverse tens of kilometers of ice with…”
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Constraints on the behavior of a descending ice probe due to force balance by Cassler, Bailey, Smith, Miles W.E., Durka, Michael J., Furst, Benjamin I., Hockman, Benjamin J., Ullman, Michael J.

“…Water is a key ingredient to the potential for life, and several icy moons, such as Europa and Enceladus, are thought to harbor a dynamic subsurface ocean of…”
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βD305A Mutant of Tryptophan Synthase Shows Strongly Perturbed Allosteric Regulation and Substrate Specificity by Ferrari, Davide, Yang, Li-Hong, Miles, Edith W, Dunn, Michael F

“…Substrate channeling in the tryptophan synthase bienzyme is regulated by allosteric interactions. Allosteric signals are transmitted via a scaffolding of…”
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Modeling a class of thermal ice probes for accessing the solar system’s ocean worlds by Durka, Michael J., Smith, Miles W.E., Ullman, Michael J., Cassler, Bailey, Otis, Richard, Cwik, Thomas A., Hockman, Benjamin J., Barry, Matthew M.

“…The subsurface oceans of icy moons in the outer solar system are prime candidates for harboring extra-terrestrial life. Missions to melt through the ice shells…”
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Investigation of Allosteric Linkages in the Regulation of Tryptophan Synthase:  The Roles of Salt Bridges and Monovalent Cations Probed by Site-Directed Mutation, Optical Spectroscopy, and Kinetics by Weber-Ban, Eilika, Hur, Oscar, Bagwell, Candy, Banik, Utpal, Yang, Li-Hong, Miles, Edith W, Dunn, Michael F

“…The tryptophan synthase bienzyme complex is the most extensively documented example of substrate channeling in which the oligomeric unit has been described at…”
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The Reaction of Yeast Cystathionine β-Synthase Is Rate-Limited by the Conversion of Aminoacrylate to Cystathionine by Jhee, Kwang-Hwan, Niks, Dimitri, McPhie, Peter, Dunn, Michael F, Miles, Edith Wilson

“…Our studies of the reaction mechanism of cystathionine β-synthase from Saccharomyces cerevisiae (yeast) are facilitated by the spectroscopic properties of the…”
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A Quasi-Experimental Assessment of the Effect of Computerizing Noncognitive Paper-and-Pencil Measurements: A Test of Measurement Equivalence by King, Wesley C, Miles, Edward W

“…Previous studies have provided evidence to question the equivalence of data gathered by different administration modes and to suggest that noncognitive…”
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The beta subunit of tryptophan synthase. Clarification of the roles of histidine 86, lysine 87, arginine 148, cysteine 170, and cysteine 230 by MILES, E. W, KAWASAKI, H, SYED ASHRAFUDDIN AHMED, MORITA, H, NAGATA, S

“…Our studies, which are aimed at understanding the catalytic mechanism of the beta subunit of tryptophan synthase from Salmonella typhimurium, use site-directed…”
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The molecular basis of substrate channeling by Miles, E W, Rhee, S, Davies, D R

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Lysine 87 in the beta subunit of tryptophan synthase that forms an internal aldimine with pyridoxal phosphate serves critical roles in transimination, catalysis, and product release by ZICHUN LU, NAGATA, S, MCPHIE, P, MILES, E. W

“…This study provides valuable insights into the functions of the lysine residue that forms an internal aldimine with pyridoxal phosphate in the beta subunit of…”
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Evidence that mutations in a loop region of the alpha-subunit inhibit the transition from an open to a closed conformation in the tryptophan synthase bienzyme complex by BRZOVIC, P. S, SAWA, Y, HYDE, C. C, MILES, E. W, DUNN, M. F

“…Rapid-scanning stopped-flow (RSSF) UV-visible spectroscopy has been used to investigate the effects of single amino acid mutations in the alpha-subunit of the…”
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The alpha subunit of tryptophan synthase. Evidence that aspartic acid 60 is a catalytic residue and that the double alteration of residues 175 and 211 in a second-site revertant restores the proper geometry of the substrate binding site by NAGATA, S, HYDE, C. C, MILES, E. W

“…Our studies, which are aimed at understanding the catalytic mechanism of the alpha subunit of tryptophan synthase from Salmonella typhimurium, use…”
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The Importance of Employee Demographic Profiles for Understanding Experiences of Work-Family Interrole Conflicts by Eagle, Bruce W., Icenogle, Marjorie L., Maes, Jeanne D., Miles, Edward W.

“…This article investigated the reported experiences of work-family interrole conflict differences between American men and women. Participants answered…”
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Characterization of the functional role of a flexible loop in the .alpha.-subunit of tryptophan synthase from Salmonella typhimurium by rapid-scanning, stopped-flow spectroscopy and site-directed mutagenesis by Brzovic, Peter S., Hyde, C. Craig, Miles, Edith W., Dunn, Michael F.

“…The function of a flexible loop (loop 6) in the alpha-subunit from the tryptophan synthase alpha 2 beta 2 bienzyme complex has been investigated utilizing…”
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Mechanism of mutual activation of the tryptophan synthase alpha and beta subunits. Analysis of the reaction specificity and substrate-induced inactivation of active site and tunnel mutants of the beta subunit by ASHRAF AHMED, S, RUVINOV, S. B, KAYASTHA, A. M, MILES, E. W

“…The origin of reaction and substrate specificity and the control of activity by protein-protein interaction are investigated using the tryptophan synthase…”
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Tryptophan synthase: A multienzyme complex with an intramolecular tunnel by Miles, Edith Wilson

“…Tryptophan synthase is a classic enzyme that channels a metabolic intermediate, indole. The crystal structure of the tryptophan synthase α2β2 complex from…”
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Site-specific mutagenesis of the alpha subunit of tryptophan synthase from Salmonella typhimurium. Changing arginine 179 to leucine alters the reciprocal transmission of substrate-induced conformational changes between the alpha and beta 2 subunits by Kawasaki, H, Bauerle, R, Zon, G, Ahmed, S A, Miles, E W

“…Arginine 179 of the alpha subunit of tryptophan synthase of Salmonella typhimurium was changed to leucine by site-directed mutagenesis. The mutant alpha…”
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