Search Results - "MIKESKA, Thomas"

DNA methylation biomarkers: cancer and beyond by Mikeska, Thomas, Craig, Jeffrey M

“…Biomarkers are naturally-occurring characteristics by which a particular pathological process or disease can be identified or monitored. They can reflect past…”
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DNA methylation biomarkers in cancer: progress towards clinical implementation by Mikeska, Thomas, Bock, Christoph, Do, Hongdo, Dobrovic, Alexander

“…Altered DNA methylation is ubiquitous in human cancers and specific methylation changes are often correlated with clinical features. DNA methylation…”
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Silencing of Irf7 pathways in breast cancer cells promotes bone metastasis through immune escape by Bidwell, Bradley N, Slaney, Clare Y, Withana, Nimali P, Forster, Sam, Cao, Yuan, Loi, Sherene, Andrews, Daniel, Mikeska, Thomas, Mangan, Niamh E, Samarajiwa, Shamith A, de Weerd, Nicole A, Gould, Jodee, Argani, Pedram, Möller, Andreas, Smyth, Mark J, Anderson, Robin L, Hertzog, Paul J, Parker, Belinda S

“…The authors identify Irf7 and associated interferon signaling as an important factor suppressing bone metastasis of breast cancers. Irf7 is lost in…”
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Optimization of Quantitative MGMT Promoter Methylation Analysis Using Pyrosequencing and Combined Bisulfite Restriction Analysis by Mikeska, Thomas, Bock, Christoph, El-Maarri, Osman, Hübner, Anika, Ehrentraut, Denise, Schramm, Johannes, Felsberg, Jörg, Kahl, Philip, Büttner, Reinhard, Pietsch, Torsten, Waha, Andreas

“…Resistance to chemotherapy is a major complication during treatment of cancer patients. Hypermethylation of the MGMT gene alters DNA repair and is associated…”
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The implications of heterogeneous DNA methylation for the accurate quantification of methylation by Mikeska, Thomas, Candiloro, Ida L M, Dobrovic, Alexander

“…DNA methylation based biomarkers have considerable potential for molecular diagnostics, both as tumor specific biomarkers for the early detection or…”
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Assessing combined methylation-sensitive high resolution melting and pyrosequencing for the analysis of heterogeneous DNA methylation by Candiloro, Ida L.M., Mikeska, Thomas, Dobrovic, Alexander

“…Heterogeneous DNA methylation leads to difficulties in accurate detection and quantification of methylation. Methylation-sensitive high resolution melting…”
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Epigenetic Downregulation of Mitogen-Activated Protein Kinase Phosphatase MKP-2 Relieves Its Growth Suppressive Activity in Glioma Cells by WAHA, Anke, FELSBERG, Jörg, WIESTLER, Otmar D, REIFENBERGER, Guido, PIETSCH, Torsten, WAHA, Andreas, HARTMANN, Wolfgang, VON DEM KNESEBECK, Anna, MIKESKA, Thomas, JOOS, Stefan, WOLTER, Marietta, KOCH, Arend, YAN, Pearlly S, ENDL, Elmar

“…Critical tumor suppression pathways in brain tumors have yet to be fully defined. Along with mutational analyses, genome-wide epigenetic investigations may…”
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Assessing alternative base substitutions at primer CpG sites to optimise unbiased PCR amplification of methylated sequences by Candiloro, Ida L M, Mikeska, Thomas, Dobrovic, Alexander

“…Determining the role of DNA methylation in various biological processes is dependent on the accurate representation of often highly complex patterns. Accurate…”
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Validation of a primer optimisation matrix to improve the performance of reverse transcription - quantitative real-time PCR assays by Mikeska, Thomas, Dobrovic, Alexander

“…The development of reverse transcription - quantitative real-time PCR (RT-qPCR) platforms that can simultaneously measure the expression of multiple genes is…”
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Epigenetic silencing of the candidate tumor suppressor gene PROX1 in sporadic breast cancer by Versmold, Beatrix, Felsberg, Jörg, Mikeska, Thomas, Ehrentraut, Denise, Köhler, Juliane, Hampl, Juergen A., Röhn, Gabriele, Niederacher, Dieter, Betz, Beate, Hellmich, Martin, Pietsch, Torsten, Schmutzler, Rita K., Waha, Andreas

“…Extensive hypermethylation and consecutive transcriptional silencing of tumorsuppressor genes have been documented in multiple tumor entities including breast…”
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Selective inhibition of proliferation in colorectal carcinoma cell lines expressing mutant APC or activated B‐Raf by Zhang, Hui‐Hua, Walker, Francesca, Kiflemariam, Sara, Whitehead, Robert H., Williams, David, Phillips, Wayne A., Mikeska, Thomas, Dobrovic, Alexander, Burgess, Antony W.

“…Tumor‐derived cell lines are indispensable tools for understanding the contribution of activated signaling pathways to the cancer phenotype and for the design…”
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Rapid analysis of heterogeneously methylated DNA using digital methylation-sensitive high resolution melting: application to the CDKN2B (p15) gene by Candiloro, Ida L M, Mikeska, Thomas, Hokland, Peter, Dobrovic, Alexander

“…Methylation-sensitive high resolution melting (MS-HRM) methodology is able to recognise heterogeneously methylated sequences by their characteristic melting…”
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RANK ( TNFRSF11A ) Is Epigenetically Inactivated and Induces Apoptosis in Gliomas by von dem Knesebeck, Anna, Felsberg, Jörg, Waha, Anke, Hartmann, Wolfgang, Scheffler, Björn, Glas, Martin, Hammes, Jennifer, Mikeska, Thomas, Yan, Pearlly S, Endl, Elmar, Simon, Matthias, Reifenberger, Guido, Pietsch, Torsten, Waha, Andreas

“…Abstract Alterations of DNA methylation play an important role in gliomas. In a genome-wide screen, we identified a CpG-rich fragment within the 5′ region of…”
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Aberrant Methylation and Reduced Expression of LHX9 in Malignant Gliomas of Childhood by Vladimirova, Valentina, Mikeska, Thomas, Waha, Andreas, Soerensen, Niels, Xu, Jingying, Reynolds, Patrick C., Pietsch, Torsten

“…High-grade gliomas (HGGs) of childhood represent approximately 7% of pediatric brain tumors. They are highly invasive tumors and respond poorly to conventional…”
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A multiplex endpoint RT-PCR assay for quality assessment of RNA extracted from formalin-fixed paraffin-embedded tissues by Takano, Elena A, Mikeska, Thomas, Dobrovic, Alexander, Byrne, David J, Fox, Stephen B

“…RNA extracted from formalin-fixed paraffin-embedded (FFPE) samples is chemically modified and degraded, which compromises its use in gene expression studies…”
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Mutations of the Wnt antagonist AXIN2 (Conductin) result in TCF‐dependent transcription in medulloblastomas by Koch, Arend, Hrychyk, Aksana, Hartmann, Wolfgang, Waha, Anke, Mikeska, Thomas, Waha, Andreas, Schüller, Ulrich, Sörensen, Nils, Berthold, Frank, Goodyer, Cynthia G., Wiestler, Otmar D., Birchmeier, Walter, Behrens, Jürgen, Pietsch, Torsten

“…Medulloblastomas (MBs) represent the most common malignant brain tumors in children. Most MBs develop sporadically in the cerebellum, but their incidence is…”
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p75NTR induces apoptosis in medulloblastoma cells by Küchler, Jan, Hartmann, Wolfgang, Waha, Anke, Koch, Arend, Endl, Elmar, Wurst, Peter, Kindler, Dagmar, Mikeska, Thomas, Waha, Andreas, Goodyer, Cynthia G., Büttner, Reinhard, Schilling, Karl, Pietsch, Torsten

“…The classic medulloblastoma (CMB) and the desmoplastic medulloblastoma (DMB) subtypes represent the major medulloblastoma variants. In contrast to CMB, DMB…”
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CpG island methylation in human lymphocytes is highly correlated with DNA sequence, repeats, and predicted DNA structure by Bock, Christoph, Paulsen, Martina, Tierling, Sascha, Mikeska, Thomas, Lengauer, Thomas, Walter, Jörn

“…CpG island methylation plays an important role in epigenetic gene control during mammalian development and is frequently altered in disease situations such as…”
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Crystal structures of O-acetylated 2-acylamino-2-deoxy- d-galactose derivatives by Mikeska, Thomas, Nieger, Martin, Mansikkamäki, Heidi, Daniels, Jörg, Kolter, Thomas

“…The X-ray structures of 1,3,4,6-tetra- O-acetyl-2-deoxy-α- d-galactopyranoside derivatives with four different 2-(acylamino) substituents have been determined…”
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Nonequivalent Gene Expression and Copy Number Alterations in High-Grade Serous Ovarian Cancers with BRCA1 and BRCA2 Mutations by GEORGE, Joshy, ALSOP, Kathryn, BOWTELL, David D, ETEMADMOGHADAM, Dariush, HONDOW, Heather, MIKESKA, Thomas, DOBROVIC, Alexander, DEFAZIO, Anna, SMYTH, Gordon K, LEVINE, Douglas A, MITCHELL, Gillian

“…High-grade serous carcinoma (HGSC) accounts for the majority of epithelial ovarian cancer deaths. Genomic and functional data suggest that approximately half…”
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