Fast decline of hematopoiesis and uncoupling protein 2 content in human liver after birth : Location of the protein in Kupffer cells

Fast decline of hematopoiesis and uncoupling protein 2 content in human liver after birth : Location of the protein in Kupffer cells

Hepatic hematopoiesis is prominent during fetal life and ceases around birth. In rodent liver, the decline of the hepatic hematopoiesis starts abruptly at birth being accompanied by a decrease of mitochondrial uncoupling protein 2 (UCP2) expression in monocytes/macrophages, whereas hepatocytes may e...

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Published in:Pediatric research Vol. 49; no. 3; pp. 440 - 447
Main Authors: BRAUNER, Petr, NIBBELINK, Maryse, KOPECKY, Jan, FLACHS, Pavel, VITKOVA, Ivana, KOPECKY, Pavel, MERTELIKOVA, Irena, JANDEROVA, Lenka, PENICAUD, Luc, CASTEILLA, Louis, PLAVKA, Richard
Format: Journal Article
Language:English
Published: Hagerstown, MD Lippincott Williams & Wilkins 01-03-2001
Subjects:
Biological and medical sciences
Down-Regulation
Embryology: invertebrates and vertebrates. Teratology
Female
Fundamental and applied biological sciences. Psychology
Hematopoiesis
Humans
Infant, Newborn
Infant, Premature
Infant, Very Low Birth Weight
Ion Channels
Kupffer Cells - cytology
Kupffer Cells - metabolism
Liver - cytology
Liver - physiology
Male
Membrane Transport Proteins
Mitochondrial Proteins
Organogenesis. Physiological fonctions
Physiological fonctions
Proteins - metabolism
Uncoupling Protein 2
Vertebrates: blood, hematopoietic organs, reticuloendothelial system
Human
Immunohistochemistry
Premature
Pathology
Kupffer cell
Newborn
Uncoupling protein
Liver
Extramedullary hematopoiesis
Hematopoietic cell
Perinatal
Evolution
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Summary:Hepatic hematopoiesis is prominent during fetal life and ceases around birth. In rodent liver, the decline of the hepatic hematopoiesis starts abruptly at birth being accompanied by a decrease of mitochondrial uncoupling protein 2 (UCP2) expression in monocytes/macrophages, whereas hepatocytes may express UCP2 only under pathologic situations. The goals of this study were to characterize hepatic hematopoiesis in humans around birth, and to identify cells expressing UCP2. Hematopoiesis was evaluated histologically in the liver of 22 newborns (mostly very premature neonates), who died between 45 min and 140 d after birth, and one fetus. UCP2 expression was characterized by Northern blots, immunoblotting, immunohistochemistry, and by in situ hybridization. The number of hematopoietic cells started to decrease rapidly at birth, irrespectively of the gestational age (23-40 wk) of neonates. A similar decline was observed for UCP2 expression, which was relatively high in fetal liver. UCP2 was detected only in myeloid cells (mainly in Kupffer cells), but not in hepatocytes, although sepsis or other pathologies occurred in the critically ill newborns. Kupffer cells represent the major site of mitochondrial UCP2 expression in the human newborn. UCP2 may be essential for the differentiation and function of macrophages and serve as a marker for these cells in human liver during the perinatal period.
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ISSN:0031-3998
1530-0447
DOI:10.1203/00006450-200103000-00022