Search Results - "MEI LIE WONG"
-
1
In vitro reconstitution of DNA replication initiated by genetic recombination: a T4 bacteriophage model for a type of DNA synthesis important for all cells
Published in Molecular biology of the cell (01-01-2019)“…Using a mixture of 10 purified DNA replication and DNA recombination proteins encoded by the bacteriophage T4 genome, plus two homologous DNA molecules, we…”
Get full text
Journal Article -
2
Centrosome Loss in the Evolution of Planarians
Published in Science (American Association for the Advancement of Science) (27-01-2012)“…The centrosome, a cytoplasmic organelle formed by cylinder-shaped centrioles surrounded by a microtubule-organizing matrix, is a hallmark of animal cells. The…”
Get full text
Journal Article -
3
Structural and functional characterizations of the proteasome-activating protein PA26 from Trypanosoma brucei
Published in The Journal of biological chemistry (26-11-1999)“…The activated 20 S proteasome, which has been found only in mammalian cells, is composed of two heptamer rings of an activator protein on each end of the 20 S…”
Get full text
Journal Article -
4
Nucleation of microtubule assembly by a γ-tubulin-containing ring complex
Published in Nature (London) (07-12-1995)“…The highly conserved protein gamma-tubulin is required for microtubule nucleation in vivo. When viewed in the electron microscope, a highly purified…”
Get full text
Journal Article -
5
A purified Drosophila septin complex forms filaments and exhibits GTPase activity
Published in The Journal of cell biology (01-05-1996)“…Septin proteins are necessary for cytokinesis in budding yeast and Drosophila and are thought to be the subunits of the yeast neck filaments. To test whether…”
Get full text
Journal Article -
6
Purification and assay of a septin complex from Drosophila embryos
Published in Methods in enzymology (1998)Get more information
Journal Article -
7
In vitro replication through nucleosomes without histone displacement
Published in Nature (London) (22-02-1990)“…A well-characterized set of proteins encoded by bacteriophage T4 replicates DNA in vitro and generates replication forks that can pass nucleosomes. The histone…”
Get full text
Journal Article -
8
Reorganization of the cytoskeleton during Drosophila oogenesis: implications for axis specification and intercellular transport
Published in Development (Cambridge) (01-08-1992)“…Inhibitor studies have implicated microtubules in at least three important developmental processes during Drosophila oogenesis: oocyte determination and growth…”
Get full text
Journal Article -
9
Regulation of clathrin assembly and trimerization defined using recombinant triskelion hubs
Published in Cell (20-10-1995)“…Clathrin polymerization into a polyhedral vesicle coat drives receptor sorting at cellular membranes during endocytosis and organelle biogenesis. To study…”
Get full text
Journal Article -
10
α5 subunit in Trypanosoma brucei proteasome can self-assemble to form a cylinder of four stacked heptamer rings
Published in Biochemical journal (01-12-1999)Get full text
Journal Article -
11
Mitotic Regulators Govern Progress through Steps in the Centrosome Duplication Cycle
Published in The Journal of cell biology (27-12-1999)“…Centrosome duplication is marked by discrete changes in centriole structure that occur in lock-step with cell cycle transitions. We show that mitotic…”
Get full text
Journal Article -
12
A Eukaryotic Cytosolic Chaperonin Is Associated with a High Molecular Weight Intermediate in the Assembly of Hepatitis B Virus Capsid, a Multimeric Particle
Published in The Journal of cell biology (01-04-1994)“…We have established a system for assembly of hepatitis B virus capsid, a homomultimer of the viral core polypeptide, using cell-free transcription-linked…”
Get full text
Journal Article -
13
The DNA replication fork can pass RNA polymerase without displacing the nascent transcript
Published in Nature (London) (04-11-1993)“…Replication proteins encoded by bacteriophage T4 generate DNA replication forks that can pass a molecule of Escherichia coli RNA polymerase moving in the same…”
Get full text
Journal Article -
14
Purification and characterization of proteasomes from Trypanosoma brucei
Published in Molecular and biochemical parasitology (01-06-1996)“…Proteasomes are multisubunit proteases that exist universally among eukaryotes. They have multiple proteolytic activities, and are believed to have important…”
Get full text
Journal Article -
15
A Transcribing RNA Polymerase Molecule Survives DNA Replication without Aborting its Growing RNA Chain
Published in Proceedings of the National Academy of Sciences - PNAS (25-10-1994)“…We have demonstrated elsewhere that a precisely placed, stalled Escherichia coli RNA polymerase ternary transcription complex (polymerase-RNA-DNA) stays on the…”
Get full text
Journal Article -
16
Simultaneous Interactions of Bacteriophage T4 DNA Replication Proteins gp59 and gp32 with Single-stranded (ss) DNA
Published in The Journal of biological chemistry (06-08-1999)“…The T4 gp59 protein is the major accessory protein of the phageâs replicative DNA helicase, gp41. gp59 helps load gp41 at DNA replication forks by promoting…”
Get full text
Journal Article -
17
α5 subunit in Trypanosoma brucei proteasome can self-assemble to form a cylinder of four stacked heptamer rings
Published in Biochemical journal (01-12-1999)“…The proteasomes have a central role in catalysing protein degradation among both prokaryotes and eukaryotes. The 20 S proteasome constitutes their catalytic…”
Get full text
Journal Article -
18
Purification and assay of gamma tubulin ring complex
Published in Methods in enzymology (1998)Get more information
Journal Article -
19
Properties of the Insulin Receptor Ectodomain
Published in Proceedings of the National Academy of Sciences - PNAS (01-10-1988)“…To study the properties of the extracellular insulin-binding domain of the human insulin receptor (hIR), we have expressed portions of the parent molecule in…”
Get full text
Journal Article -
20
A Different Sort of Mott Cell
Published in Proceedings of the National Academy of Sciences - PNAS (15-12-1992)“…NYC is a B lymphoma cell line derived from B/W mice. Upon fusion of NYC cells with a plasmacytoma, which itself produces no immunoglobulin, the resulting NYCH…”
Get full text
Journal Article