Application of bacterial magnetic particles as novel DNA carriers for Ballistic transformation of a marine cyanobacterium

Bacterial magnetite particles (BMPs) of 50 to 100 nm diam were used as DNA carriers for the ballistic transformation of the marine cyanobacterium Synechococcus. BMPs were bombarded into the cyanobacterial cells at several bombardment velocities using a particle gun. Successful transformation and gen...

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Bibliographic Details
Published in:	Biotechnology techniques Vol. 9; no. 5; pp. 355 - 360
Main Authors:	TAKEYAMA, H, YAMZAWA, A, NALAMIURA C, MATDSUNAGA, T
Format:	Journal Article
Language:	English
Published:	London Chapman and Hall 01-05-1995
Subjects:	Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Magnetospirillum Marine Methods. Procedures. Technologies Synechococcus Vectors (cloning, transfer, expression). Insertion sequences and transposons Performance evaluation Microprojectile bombardment Magnetite Transfection Genetic transformation Cyanobacteria Synechococcus Bacteria Gene expression Velocity Operating conditions
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Summary:	Bacterial magnetite particles (BMPs) of 50 to 100 nm diam were used as DNA carriers for the ballistic transformation of the marine cyanobacterium Synechococcus. BMPs were bombarded into the cyanobacterial cells at several bombardment velocities using a particle gun. Successful transformation and gene expression were confirmed by Southern hybridization and CAT assay, respectively. The BMPs were also observed in the cyanobacterial cells by transmission electron microscopy. These results suggested that BMPs can be used as carriers for introducing DNA into bacterial cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0951-208X 1573-6784
DOI:	10.1007/BF00638870