Application and assessment of a dipstick assay in the diagnosis of hydatidosis and trichinosis

Application and assessment of a dipstick assay in the diagnosis of hydatidosis and trichinosis

The aim of the present work was to apply and evaluate a dipstick assay for the serodiagnosis of human hydatidosis as well as human and experimental trichinosis using camel hydatid cyst fluid (HCF) and Trichinella spiralis muscle larval (TSML) antigens, respectively, and compare this to enzyme-linked...

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Bibliographic Details
Published in:Parasitology research (1987) Vol. 93; no. 2; pp. 87 - 95
Main Authors: AL-SHERBINY, Maged M, FARRAG, Abd-Al Magid M. K, FAYAD, Mohamed H, MAKLED, Mohamed K, TAWFEEK, Gihan M, ALI, Nehad M. S
Format: Journal Article
Language:English
Published: Berlin Springer 01-06-2004
Subjects:
Adolescent
Adult
Aged
Animals
Antibodies, Helminth - blood
Biological and medical sciences
Blotting, Western
Child
Child, Preschool
Echinococcosis - diagnosis
Echinococcosis - parasitology
Echinococcus - immunology
Enzyme-Linked Immunosorbent Assay
Female
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Humans
Invertebrates
Male
Mice
Middle Aged
Reagent Strips
Sensitivity and Specificity
Trichinella spiralis - immunology
Trichinellosis - diagnosis
Trichinellosis - parasitology
Infection
Nematode disease
Parasite
Parasitosis
Diagnosis
Helminthiasis
Trichinosis
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Summary:The aim of the present work was to apply and evaluate a dipstick assay for the serodiagnosis of human hydatidosis as well as human and experimental trichinosis using camel hydatid cyst fluid (HCF) and Trichinella spiralis muscle larval (TSML) antigens, respectively, and compare this to enzyme-linked immunoelectrotransfer blot (EITB) and Falcon assay screening test-enzyme-linked immunosorbent assay (FAST-ELISA). Sera samples were collected from patients with confirmed hydatidosis and trichinosis and with other parasitic diseases as well as from normal healthy individuals. Also, sera samples were collected from mice experimentally infected with T. spiralis which were sacrificed at different time points post-infection (PI). HCF and TSML antigens were used in EITB after separation and characterization of their antigenic components using 5-22.5% sodium dodecyl sulphate-polyacrylamide gel electrophoresis under non-reducing condition. For the diagnosis of hydatidosis, the sensitivity, specificity and diagnostic accuracy of the dipstick assay and EITB were 100, 91.4 and 95.1% while those of FAST-ELISA were 96.2, 100 and 98.4%, respectively. For the diagnosis of human trichinosis, the sensitivity, specificity and diagnostic accuracy of the dipstick assay and EITB were 100% while those of FAST-ELISA were 85.7%. FAST-ELISA proved to be more sensitive in the early diagnosis of experimental T. spiralis infection (100% sensitivity from the second week PI) than the dipstick and EITB (100% sensitivity from the third week PI). All tests retained their sensitivity till the 12th week PI. Since the dipstick assay is extremely easy to perform with a visually interpreted result within 15 min, in addition to being both sensitive and specific, the test could be an acceptable alternative for use in clinical laboratories lacking specialized equipment and the technological expertise needed for EITB and FAST-ELISA.
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ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-004-1076-x