Conditioned media and DMSO enhance the cryopreservation of bovine adipose tissue-derived mesenchymal stem cells

Mesenchymal stem cells derived from adipose tissue (AD-MSCs) show great potential for repro ductive biotechnology in the livestock sector. However, enzyme-based isolation of MSCs is expensive and time-consuming, so it is still rarely done, especially for applications in the livestock sector in de ve...

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Bibliographic Details
Published in:	Journal of the Indonesian Tropical Animal Agriculture Vol. 49; no. 2; pp. 181 - 190
Main Authors:	S. Irfan, S. Suyatno, H. Zulfiqar, D. A. Lestari, A. Hafid, T. Kostaman, H. Herdis, T. P. Priyatno, P. I. Sitaresmi, M. F. Hudaya, F. B. I. Lupitasari, M. Pangestu
Format:	Journal Article
Language:	English
Published:	Diponegoro University, Faculty of Animal and Agricultural Sciences 01-06-2024
Subjects:	adipose tissue cryopreservation cryoprotective agents livestock mesenchymal stem cells proliferation viability
Online Access:	Get full text
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Summary:	Mesenchymal stem cells derived from adipose tissue (AD-MSCs) show great potential for repro ductive biotechnology in the livestock sector. However, enzyme-based isolation of MSCs is expensive and time-consuming, so it is still rarely done, especially for applications in the livestock sector in de veloping countries. So, MSCs must be cryopreserved with an efficient cryoprotective agent to be stored and reproduced in various laboratories after isolation. This study was aimed to optimize the cryopreser vation media for adipose-derived MSCs in cattle. This study evaluated the viability, proliferation, and morphology of AD-MSCs. The results of this study indicate that a combination of 10% DMSO, 45% DMEM, and 45% conditioned media significantly improves post-thaw viability, proliferation, and sur vival as compared to other mediums. Furthermore, AD-MSCs cryopreserved in this medium exhibit similar morphology as fresh cells. These findings suggest that the optimized cryopreservation medium can enhance the quality and safety of AD-MSCs for clinical applications in the livestock industry.
ISSN:	2087-8273 2460-6278
DOI:	10.14710/jitaa.49.2.181-190