Search Results - "Mähönen, Anssi"

A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells by Kärkkäinen, Hanna-Riikka, Lesch, Hanna P, Määttä, Antti I, Toivanen, Pyry I, Mähönen, Anssi J, Roschier, Miia M, Airenne, Kari J, Laitinen, Olli H, Ylä-Herttuala, Seppo

“…Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous…”
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Baculovirus-mediated vascular endothelial growth factor-D(ΔNΔC) gene transfer induces angiogenesis in rabbit skeletal muscle by Heikura, Tommi, Nieminen, Tiina, Roschier, Miia M, Karvinen, Henna, Kaikkonen, Minna U, Mähönen, Anssi J, Lesch, Hanna P, Rissanen, Tuomas T, Laitinen, Olli H, Airenne, Kari J, Ylä-Herttuala, Seppo

“…Occluded arteries and ischemic tissues cannot always be treated by angioplasty, stenting or by-pass-surgery. Under such circumstances, viral gene therapy may…”
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Short and Long-Term Effects of hVEGF-A165 in Cre-Activated Transgenic Mice by Leppänen, Pia, Kholová, Ivana, Mähönen, Anssi J, Airenne, Kari, Koota, Suvi, Mansukoski, Hannu, Närväinen, Johanna, Wirzenius, Maria, Alhonen, Leena, Jänne, Juhani, Alitalo, Kari, Ylä-Herttuala, Seppo

“…We have generated a transgenic mouse where hVEGF-A165 expression has been silenced with loxP-STOP fragment, and we used this model to study the effects of…”
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Enhanced Gene Delivery by Avidin-Displaying Baculovirus by Räty, Jani K, Airenne, Kari J, Marttila, Ari T, Marjomäki, Varpu, Hytönen, Vesa P, Lehtolainen, Pauliina, Laitinen, Olli H, Mähönen, Anssi J, Kulomaa, Markku S, Ylä-Herttuala, Seppo

“…Flexible alteration of virus surface properties would be beneficial for enhanced and targeted gene delivery. A useful approach could be based on a…”
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Baculovirus capsid display: a novel tool for transduction imaging by Kukkonen, Sari P., Airenne, Kari J., Marjomäki, Varpu, Laitinen, Olli H., Lehtolainen, Pauliina, Kankaanpää, Pasi, Mähönen, Anssi J., Räty, Jani K., Nordlund, Henri R., Oker-Blom, Christian, Kulomaa, Markku S., Ylä-Herttuala, Seppo

“…Baculoviruses are enveloped insect viruses that can carry large quantities of foreign DNA in their genome. Baculoviruses have proved to be very promising gene…”
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A multipurpose vector system for the screening of libraries in bacteria, insect and mammalian cells and expression in vivo by Laitinen, Olli H., Airenne, Kari J., Hytönen, Vesa P., Peltomaa, Erik, Mähönen, Anssi J., Wirth, Thomas, Lind, Miia M., Mäkelä, Kari A., Toivanen, Pyry I., Schenkwein, Diana, Heikura, Tommi, Nordlund, Henri R., Kulomaa, Markku S., Ylä-Herttuala, Seppo

“…We have constructed a novel tetra-promoter vector (pBVboostFG) system that enables screening of gene/cDNA libraries for functional genomic studies. The vector…”
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In vivo application and tracking of baculovirus by Airenne, Kari J, Makkonen, Kaisa-Emilia, Mähönen, Anssi J, Ylä-Herttuala, Seppo

“…Baculoviruses are safe and high-capacity vectors for gene delivery which have matured from the initial successful experiments performed in liver cells into…”
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Transduction of vertebrate cells with recombinant baculovirus by Airenne, Kari J, Laitinen, Olli H, Mähönen, Anssi J, Ylä-Herttuala, Seppo

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Adeno-associated virus-mediated gene transfer of a secreted decoy human macrophage scavenger receptor reduces atherosclerotic lesion formation in LDL receptor knockout mice by Jalkanen, Johanna, Leppänen, Pia, Pajusola, Katri, Närvänen, Outi, Mähönen, Anssi, Vähäkangas, Elisa, Greaves, David R, Büeler, Hansruedi, Ylä-Herttuala, Seppo

“…Macrophage scavenger receptors (MSR) promote atherosclerotic lesion formation, and modulation of MSR activity has been shown to influence atherosclerosis…”
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Optimized self-excising Cre-expression cassette for mammalian cells by Mähönen, Anssi J, Airenne, Kari J, Lind, Miia M, Lesch, Hanna P, Ylä-Herttuala, Seppo

“…We observed that overexpression of Cre recombinase in 293 T cells has toxic effects and that the chicken β-actin promoter is active in Escherichia coli,…”
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Coxsackievirus B3 VLPs purified by ion exchange chromatography elicit strong immune responses in mice by Koho, Tiia, Koivunen, Minni R.L., Oikarinen, Sami, Kummola, Laura, Mäkinen, Selina, Mähönen, Anssi J., Sioofy-Khojine, Amirbabak, Marjomäki, Varpu, Kazmertsuk, Artur, Junttila, Ilkka, Kulomaa, Markku S., Hyöty, Heikki, Hytönen, Vesa P., Laitinen, Olli H.

“…•Scalable chromatographic purification method for CVB3 VLPs and virus was developed.•High-quality CVB3 VLPs and virus were achieved.•CVB3 VLPs induced strong…”
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Preparation of recombinant baculoviruses with the BVboost system by Airenne, Kari J, Laitinen, Olli H, Mähönen, Anssi J, Ylä-Herttuala, Seppo

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TiO2 coating promotes human mesenchymal stem cell proliferation without the loss of their capacity for chondrogenic differentiation by Kaitainen, Salla, Mähönen, Anssi J, Lappalainen, Reijo, Kröger, Heikki, Lammi, Mikko J, Qu, Chengjuan

“…Human mesenchymal stem cells (hMSCs) are used in applications, which may require a large amount of cells; therefore, efficient expansion of the cells is…”
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Baculovirus is an efficient vector for the transduction of the eye: comparison of baculovirus- and adenovirus-mediated intravitreal vascular endothelial growth factor D gene transfer in the rabbit eye by Kinnunen, Kati, Kalesnykas, Giedrius, Mähönen, Anssi J., Laidinen, Svetlana, Holma, Liisa, Heikura, Tommi, Airenne, Kari, Uusitalo, Hannu, Ylä-Herttuala, Seppo

“…Background The present study aimed to determine the efficiency and safety of baculovirus‐mediated intravitreal gene transfer in rabbit eye and to compare its…”
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Post-transcriptional regulatory element boosts baculovirus-mediated gene expression in vertebrate cells by Mähönen, Anssi J., Airenne, Kari J., Purola, Sanna, Peltomaa, Erik, Kaikkonen, Minna U., Riekkinen, Mervi S., Heikura, Tommi, Kinnunen, Kati, Roschier, Miia M., Wirth, Thomas, Ylä-Herttuala, Seppo

“…Baculoviruses can express transgenes in a wide range of vertebrate cells. However, in some cells transgene expression is weak. To enhance transgene expression,…”
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Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery by Mähönen, Anssi J., Makkonen, Kaisa-Emilia, Laakkonen, Johanna P., Ihalainen, Teemu O., Kukkonen, Sari P., Kaikkonen, Minna U., Vihinen-Ranta, Maija, Ylä-Herttuala, Seppo, Airenne, Kari J.

“…Baculoviruses can express transgenes under mammalian promoters in a wide range of vertebrate cells. However, the success of transgene expression is dependent…”
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Baculovirus-mediated vascular endothelial growth factor-DΔNΔC gene transfer induces angiogenesis in rabbit skeletal muscle by Heikura, Tommi, Nieminen, Tiina, Roschier, Miia M., Karvinen, Henna, Kaikkonen, Minna U., Mähönen, Anssi J., Lesch, Hanna P., Rissanen, Tuomas T., Laitinen, Olli H., Airenne, Kari J., Ylä-Herttuala, Seppo

“…Background Occluded arteries and ischemic tissues cannot always be treated by angioplasty, stenting or by‐pass‐surgery. Under such circumstances, viral gene…”
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Short and long-term effects of hVEGF-A(165) in Cre-activated transgenic mice by Leppänen, Pia, Kholová, Ivana, Mähönen, Anssi J, Airenne, Kari, Koota, Suvi, Mansukoski, Hannu, Närväinen, Johanna, Wirzenius, Maria, Alhonen, Leena, Jänne, Juhani, Alitalo, Kari, Ylä-Herttuala, Seppo

“…We have generated a transgenic mouse where hVEGF-A(165) expression has been silenced with loxP-STOP fragment, and we used this model to study the effects of…”
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Baculoviruses mediate efficient gene expression in a wide range of vertebrate cells by Airenne, Kari J, Makkonen, Kaisa-Emilia, Mähönen, Anssi J, Ylä-Herttuala, Seppo

“…Baculovirus expression vector system (BEVS) is well known as a feasible and safe technology to produce recombinant (re-)proteins in a eukaryotic milieu of…”
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Adenovirus‐mediated VEGF‐A gene transfer induces bone formation in vivo by Hiltunen, Mikko Olavi, Ruuskanen, Markku, Huuskonen, Jouni, Mähönen, Anssi Juhani, Ahonen, Mari, Rutanen, Juha, Kosma, Veli-Matti, Mahonen, Anitta, Kröger, Heikki, Ylä-Herttuala, Seppo

“…ABSTRACT Osteoporosis is a major problem in elderly population. We tested the hypothesis whether vascular endothelial growth factor (VEGF‐A) gene transfer is…”
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