Search Results - "Lysogorskaia, E N"

A new approach to the use of fluorogenic dinitrophenyl-containing substrates for determining the proteolytic activity of aspartyl proteinases by Goptar', I A, Balandina, G N, Lysogorskaia, E N, Filippova, I Iu

“…A method for the determination of proteolytic activity of aspartyl proteinases using known colored fluorogenic substrates was developed. The technique utilizes…”
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Chemoenzymatic synthesis of new fluorogenous substrates for cysteine proteases of the papain family by Semashko, T A, Lysogorskaia, E N, Oksenoĭt, E S, Bacheva, A V, Filippova, I Iu

“…A chemoenzymatic syntheses was developed for new highly specific fluorogenic substrates for cysteine proteases of the papain family, Abz-Phe-Ala-pNA (I) and…”
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Flu virion as a substrate for proteolytic enzymes by Smirnova, Iu A, Kordiukova, L V, Serebriakova, M V, Filippova, I Iu, Lysogorskaia, E N, Baratova, L A

“…The proteolysis of flu virions of the strain A/Puerto Rico/8/34 (subtype H1N1) by enzymes of various classes was studied to develop an approach to the study of…”
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Procedure for obtaining and catalytic properties of trypsin immobilized in cryogels of polyvinyl alcohol by Lysogorskaia, E N, Rosliakova, T V, Beliaeva, A V, Bacheva, A V, Lozinskiĭ, V I, Filippova, I Iu

“…Commercial preparations of trypsin, varying in activity, were immobilized in a cryogel of polyvinyl alcohol, activated by dialdehydes (terephthalic, succinic,…”
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Modified proteinases in peptide synthesis in organic media by Filippova, I Iu, Lysogorskaia, E N

“…We showed that modified proteases could catalyze synthesis of a wide variety of peptides of various lengths and structures both in solution and on solid phase…”
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Biocatalytic properties of thermolysin immobilized on polyvinyl alcohol cryogel by Beliaeva, A V, Smirnova, Iu A, Lysogorskaia, E N, Oksenoĭt, E S, Timofeeva, A V, Lozinskiĭ, V I, Filippova, I Iu

“…Preparations with different contents of thermolysin were obtained by the immobilization of the enzyme on granulated polyvinyl alcohol cryogel. Their activity…”
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Properties of post-proline cleaving enzymes from Tenebrio molitor by Goptar', I A, Kulemzina, I A, Filippova, I Iu, Lysogorskaia, E N, Oksenoĭt, E S, Zhuzhikov, D P, Dunaevskiĭ, Ia E, Belozerskiĭ, M A, Elpidina, E N

“…Two post-proline cleaving enzymes PRE1 and PRE2 with molecular masses of 101 and 62 kDa, respectively, capable of hydrolyzing Z-AlaAlaPro-pNA were isolated for…”
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Native and modified subtilisin Karlsberg as an effective catalyst of peptide bond formation in organic media by Anikina, O M, Semashko, T A, Oksenoĭt, E S, Lysogorskaia, E N, Filippova, I Iu

“…The activity and stability of native subtilisin Karlsberg and subtilisin 72 and their complexes with sodium dodecyl sulfate (SDS) in organic solvents were…”
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Peptide synthesis in organic media with the use of subtilisin 72 immobilized on a poly(vinyl alcohol) cryogel by Beliaeva, A V, Bacheva, A V, Oksenoĭt, E S, Lysogorskaia, E N, Lozinskiĭ, V I, Filippova, I Iu

“…Subtilisin 72 serine protease (EC 3.4.21.14) immobilized on a poly(vinyl alcohol) cryogel was used as a catalyst in the syntheses of N-protected peptide…”
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The enzymatic segment condensation of peptides on a solid phase in organic medium by Kolobanova, S V, Filippova, I Iu, Lysogorskaia, E N, Bacheva, A V, Oksenoĭt, E S, Stepanov, V M

“…The segment condensation of peptides on a solid phase (Aminosilochrom) in organic medium catalyzed by a subtilisin complex with sodium dodecylsulfate was…”
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Segmented condensation of peptides on a solid phase using subtilisin complexed with sodium dodecyl sulfate by Kolobanova, S V, Filippova, I Iu, Lysogorskaia, E N, Getun, I V, Bacheva, A V, Stepanov, V M

“…The subtilisin-sodium dodecyl sulfate complex was shown to catalyze the coupling of peptide segments on a solid phase in organic medium. By a two-stage enzymic…”
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Peptide synthesis catalyzed by subtilisin-72 in organic solvents by Getun, I V, Filippova, I Iu, Lysogorskaia, E N, Bacheva, A V, Oksenoĭt, E S

“…The solubility, stability, and activity of native subtilisin 72 and of its complex with SDS were comparatively studied in a number of polar organic solvents…”
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Synthesis of three- and tetrapeptides catalyzed by subtilisin suspensions in organic solvents by Getun, I V, Filippova, I Iu, Lysogorskaia, E N, Kolobanova, S V, Oksenoĭt, E S, Anisimova, V V, Stepanov, V M

“…The behavior of subtilisin 72 in some aprotic solvents (acetonitrile, dioxane, and tetrahydrofurane) was studied. The enzyme was shown to be partially soluble…”
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A study of aspartyl proteases using intramolecularly quenched fluorogenic peptide substrates by Filippova, I Iu, Lysogorskaia, E N, Lavrenova, G I, Oksenoĭt, E S, Suvorov, L I, Starovoĭtova, V V

“…A series of fluorogenic tetra-, penta-, and hexapeptide substrates of the general structure Abz-X-Phe-Phe-Y-Ded (or -pNa in place of -Ded), where X = Ala,…”
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Synthesis of peptides in organic solvents catalyzed by pepsin by Anisimova, V V, Lysogorskaia, E N, Filippova, I Iu, Oksenoĭt, E S, Stepanov, V M

“…The porcine pepsin immobilized on inorganic supports catalyzes the peptide bond formation in organic solvents. Dependence of the peptide bond formation between…”
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Pepsin in the enzymatic synthesis of esters and n-nitroanilide peptides by Lysogorskaia, E N, Filippova, I Iu, Abdel' Malak, K A, Lavrenova, G I, Anisimova, V V, Oksenoĭt, E S, Stepanov, V M

“…Pepsin was shown to catalyze synthesis of esters or p-nitroanilides tri-, tetra-, penta- and hexapeptides of general formula Z-X-Y-B, where X = Ala-Phe,…”
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Co-precipitation of pepsin with products from the enzymatic synthesis of peptides as a factor limiting the effectiveness of the enzyme by Filippova, I Iu, Lysogorskaia, E N, Anisimova, V V, Abdel' Malak, K A, Lavrenova, G I, Oksenoĭt, E S, Stepanov, V M

“…Porcine pepsin behaviour during the synthesis of peptide p-nitroanilides and esters has been studied. In many cases, especially when long-chain peptides, such…”
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New fluorescent substrates for metalloendopeptidases with internal quenching of fluorescence by Filippova, I Iu, Lysogorskaia, E N, Oksenoĭt, E S, Troshchenkova, E P, Stepanov, V M

“…p-Nitroanilides of antranyloyltripeptides of the general structure Abz-Ala-Ala-P'1-pNA (P'1 = Phe, Leu, Ile, Val) containing intramolecularly quenched…”
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Serine proteinase from the archaebacterium Halobacterium mediterranei--an analog of eubacterium subtilisin by Rudenskaia, G N, Revina, L P, Griaznova, Iu B, Lysogorskaia, E N, Oksenoĭt, E S, Filippova, I Iu, Stepanov, V M, Ivanova, I I

“…A homogeneous serine proteinase was isolated from cultural filtrates of the extreme halophilic bacteria Halobacterium mediterranei 1538 using affinity…”
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Isolation and characteristics of Bacillus megaterium metalloproteinase by Morozova, I P, Chestukhina, G G, Bormatova, M E, Gololobov, M Iu, Ivanova, N M, Lysogorskaia, E N, Filippova, I Iu, Khodova, O M, Timokhina, E A, Stepanov, V M

“…Stepwise application of affinity chromatography on bacitracin-silochrome, gel filtration on Acrylex P-10, rechromatography on bacitracin-Sepharose 4B and gel…”
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