Latent equid herpesviruses 1 and 4: detection and distinction using the polymerase chain reaction and co-cultivation from lymphoid tissues

The 1 Royal Veterinary College, Royal College Street, London NW1 0TU and 2 MRC Collaborative Centre, 1-3 Burtonhole Lane, Mill Hill, London NW7 1AD, U.K. The polymerase chain reaction (PCR) and co-cultivation were used to identify the lymphoreticular system as the site of latency of equid herpesviru...

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Bibliographic Details
Published in:	Journal of general virology Vol. 73; no. 2; pp. 261 - 268
Main Authors:	Welch, Hazel M, Bridges, C. Gordon, Lyon, Allyson M, Griffiths, Lyn, Edington, Neil
Format:	Journal Article
Language:	English
Published:	Reading Soc General Microbiol 01-02-1992 Society for General Microbiology
Subjects:	Acute Disease Animals Base Sequence Biological and medical sciences DNA, Viral - analysis DNA, Viral - chemistry Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Herpesviridae - genetics Herpesviridae - isolation & purification Herpesviridae Infections - microbiology Herpesviridae Infections - veterinary Herpesvirus 1, Equid - genetics Herpesvirus 1, Equid - isolation & purification Horse Diseases - microbiology Horses Leukocytes - microbiology Lymphoid Tissue - microbiology Microbiology Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Respiratory System - microbiology Restriction Mapping Techniques used in virology Virology Equine herpesvirus 4 Equine herpesvirus 1 Herpesviridae Alphaherpesvirinae Method Lymphoid tissue Infection Virus Polymerase chain reaction Experimental disease DNA Viral disease Detection
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Summary:	The 1 Royal Veterinary College, Royal College Street, London NW1 0TU and 2 MRC Collaborative Centre, 1-3 Burtonhole Lane, Mill Hill, London NW7 1AD, U.K. The polymerase chain reaction (PCR) and co-cultivation were used to identify the lymphoreticular system as the site of latency of equid herpesvirus I (EHV-1). Primers for PCR were designed from aligned nucleotide sequences of the glycoprotein gB genes to amplify the same region of both the EHV-1 and EHV-4 genomes. Subsequent restriction digests using specific enzymes distinguished the amplified fragments of the EHV-1 genome from those of the EHV-4 genome. Ten weeks following an experimental infection of five ponies with EHV-1, latent virus was detected by PCR and recovered by co-cultivation, predominantly from lymphoid tissues draining the respiratory tract. Significantly, latent EHV-1 also persisted in peripheral blood leukocytes (PBL). Latent EHV-4, presumably from a preceding natural infection, was also detected in some tissues, including PBL, from all animals. Of additional interest was the recovery of EHV-1 and -4 only in the presence of the ubiquitous EHV-2. Received 18 June 1991; accepted 30 October 1991.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0022-1317 1465-2099
DOI:	10.1099/0022-1317-73-2-261