Search Results - "Lye, G.J."

On the fluid dynamics of a laboratory scale single-use stirred bioreactor by Odeleye, A.O.O., Marsh, D.T.J., Osborne, M.D., Lye, G.J., Micheletti, M.

“…The commercial success of mammalian cell-derived recombinant proteins has fostered an increase in demand for novel single-use bioreactor (SUB) systems that…”
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Impact of cofactor-binding loop mutations on thermotolerance and activity of E. coli transketolase by Morris, P., Rios-Solis, L., García-Arrazola, R., Lye, G.J., Dalby, P.A.

“…[Display omitted] •Transketolase variants with increased thermostability have been obtained by engineering cofactor-binding loops towards those found in a…”
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Immobilised enzyme microreactor for screening of multi-step bioconversions: Characterisation of a de novo transketolase-ω-transaminase pathway to synthesise chiral amino alcohols by Matosevic, S., Lye, G.J., Baganz, F.

“…Complex molecules are synthesised via a number of multi-step reactions in living cells. In this work, we describe the development of a continuous flow…”
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Modelling and optimisation of the one-pot, multi-enzymatic synthesis of chiral amino-alcohols based on microscale kinetic parameter determination by Rios-Solis, L., Morris, P., Grant, C., Odeleye, A.O.O., Hailes, H.C., Ward, J.M., Dalby, P.A., Baganz, F., Lye, G.J.

“…Advances in synthetic biology are facilitating the de novo design of complex, multi-step enzymatic conversions for industrial organic synthesis. This work…”
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Quantification of power consumption and oxygen transfer characteristics of a stirred miniature bioreactor for predictive fermentation scale-up by Gill, N.K, Appleton, M, Baganz, F, Lye, G.J

“…Miniature parallel bioreactors are becoming increasingly important as tools to facilitate rapid bioprocess design. Once the most promising strain and culture…”
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Evaluation of ionic liquids as dispersed phase during the production of lactones with E. coli in a three phase partitioning bioreactor by Melgarejo-Torres, R., Castillo-Araiza, C.O., López-Ordaz, P., Calleja-Castañeda, N.V., Cano-Velasco, J.L., Camacho-Ruíz, R.M., Lye, G.J., Huerta-Ochoa, S.

“…•Conversion at inhibitory conditions was improved with the use of the IL.•Parameters (Kpp, Kps, ksA, kpA, kin, kLa) were used to select the most proper…”
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Room-temperature ionic liquids as replacements for organic solvents in multiphase bioprocess operations by Cull, S.G., Holbrey, J.D., Vargas-Mora, V., Seddon, K.R., Lye, G.J.

“…Organic solvents are widely used in a range of multiphase bioprocess operations including the liquid–liquid extraction of antibiotics and two‐phase…”
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High throughput screening of monoamine oxidase (MAO-N-D5) substrate selectivity and rapid kinetic model generation by Rios-Solis, L., Mothia, B., Yi, S., Zhou, Y., Micheletti, M., Lye, G.J.

“…•Simultaneous microscale substrate screening and kinetic characterization.•Selective oxidation of secondary amines in 8 and 9 carbon rings.•Formation of new…”
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Impact of aeration strategies on fed-batch cell culture kinetics in a single-use 24-well miniature bioreactor by Betts, J.P.J., Warr, S.R.C., Finka, G.B., Uden, M., Town, M., Janda, J.M., Baganz, F., Lye, G.J.

“…•This paper is interested in small scale bioreactors for mammalian cell culture.•We perform a detailed engineering characterisation of the μ24 minibioreactor…”
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Design and characterisation of a miniature stirred bioreactor system for parallel microbial fermentations by Gill, N.K., Appleton, M., Baganz, F., Lye, G.J.

“…The establishment of a high productivity microbial fermentation process requires the experimental investigation of many interacting variables. In order to…”
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Kinetic mathematical model for ketone bioconversion using Escherichia coli TOP10 pQR239 by Melgarejo-Torres, R., Castillo-Araiza, O., López-Ordaz, P., Torres-Martínez, D., Gutiérrez-Rojas, M., Lye, G.J., Huerta-Ochoa, S.

“…•A non-reported pseudo intrinsic kinetic model was developed on basic reactions.•The reaction mechanism proposed followed the formalism of…”
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Non-linear kinetic modelling of reversible bioconversions: Application to the transaminase catalyzed synthesis of chiral amino-alcohols by Rios-Solis, L., Bayir, N., Halim, M., Du, C., Ward, J.M., Baganz, F., Lye, G.J.

“…► Created a rapid methodology to determine kinetic parameters for complex enzymatic reactions. ► Used statistical analysis to allow selection of the most…”
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Fluid mixing in shaken bioreactors: Implications for scale-up predictions from microlitre-scale microbial and mammalian cell cultures by Micheletti, M., Barrett, T., Doig, S.D., Baganz, F., Levy, M.S., Woodley, J.M., Lye, G.J.

“…Pressures on pharmaceutical companies to speed bioprocess development have led to significant interest in small scale, parallel experimentation. A particular…”
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Mass transfer coefficient determination in three biphasic systems (water–ionic liquid) using a modified Lewis cell by Melgarejo-Torres, R., Torres-Martínez, D., Castillo-Araiza, C.O., Arriaga-Juárez, C., Gutiérrez-Rojas, M., Esponda-Aguilar, P., Aroca, G., Lye, G.J., Huerta-Ochoa, S.

“…► We determine mass transfer coefficients in three biphasic water–ionic liquid systems. ► We used a modified Lewis cell to determine global mass transfer…”
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Regime analysis of a Baeyer–Villiger bioconversion in a three-phase (air–water–ionic liquid) stirred tank bioreactor by Melgarejo-Torres, R., Torres-Martínez, D., Gutiérrez-Rojas, M., Gómez de Jesús, A., Lye, G.J., Huerta-Ochoa, S.

“…► We conduct a regime analysis on a three-phase stirred tank bioreactor. ► We studied a bioconversion process using [MeBuPyrr][BTA] as the dispersed phase. ►…”
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Study of a novel tube forming method for preparing engineered blood vessels by Eames, I., Chau, G., Landeryou, M., Town, M., Levy, M.S., Lye, G.J., Hoare, M., Mason, C.

“…A novel tube forming process for preparing engineered blood vessels is described. The process involves charging a vertical cylindrical tube with a matrix…”
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Use of microwells to investigate the effect of quorum sensing on growth and antigen production in Bacillus anthracis Sterne 34F2 by Mukhopadhyay, T.K, Allison, N, Charlton, S, Ward, J, Lye, G.J

“…Aim: The aim of this study was to investigate the role of quorum sensing in Bacillus anthracis growth and toxin production. Methods and Results: A microwell…”
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Scale-down prediction of industrial scale pleated membrane cartridge performance by Brown, A.I., Titchener-Hooker, N.J., Lye, G.J.

“…Flat‐sheet membrane discs represent the current standard format used for experimental prediction of the scale‐up of normal flow filtration processes. Use of…”
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Flux and transmission characteristics of a vibrating microfiltration system operated at high biomass loading by Postlethwaite, J., Lamping, S.R., Leach, G.C., Hurwitz, M.F., Lye, G.J.

“…Conventional, crossflow microfiltration systems rely on high liquid crossflow velocities to generate shear at the liquid-membrane surface. Shear is necessary…”
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Scale-up of Escherichia coli growth and recombinant protein expression conditions from microwell to laboratory and pilot scale based on matched kLa by Islam, R.S, Tisi, D, Levy, M.S, Lye, G.J

“…Fermentation optimization experiments are ideally performed at small scale to reduce time, cost and resource requirements. Currently microwell plates (MWPs)…”
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