Mutants of T7 RNA polymerase that are able to synthesize both RNA and DNA

Mutants of T7 RNA polymerase that are able to synthesize both RNA and DNA

A mutant T7 RNA polymerase (T7 RNAP) having two amino-acid substitutions (Y639F and S641A) is altered in its specificity towards nucleotide substrates, but is not affected in the specificity of its interaction with promoter and terminator sequences. The mutant enzyme gains the ability to utilize dNT...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters Vol. 369; no. 2; pp. 165 - 168
Main Authors: Kostyuk, D.A., Dragan, S.M., Lyakhov, D.L., Rechinsky, V.O., Tunitskaya, V.L., Chernov, B.K., Kochetkov, S.N.
Format: Journal Article
Language:English
Published: England Elsevier B.V 07-08-1995
Subjects:
Amino Acid Sequence
Base Sequence
DNA - biosynthesis
DNA polymerizing activity
DNA, Single-Stranded - metabolism
DNA, Superhelical - metabolism
DNA-Directed DNA Polymerase - metabolism
DNA-Directed RNA Polymerases - genetics
DNA-Directed RNA Polymerases - metabolism
dNTP utilization
Molecular Sequence Data
Mutagenesis
Mutation
Nucleotides - metabolism
Plasmids - metabolism
RNA - biosynthesis
Substrate Specificity
T7 RNA polymerase
Viral Proteins
T7 RNA polymerase
dNTP utilization
Mutagenesis
DNA polymerizing activity
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A mutant T7 RNA polymerase (T7 RNAP) having two amino-acid substitutions (Y639F and S641A) is altered in its specificity towards nucleotide substrates, but is not affected in the specificity of its interaction with promoter and terminator sequences. The mutant enzyme gains the ability to utilize dNTPs and catalyze RNA and DNA synthesis from circular supercoiled plasmid DNA. DNA synthesis can also be initiated from a single stranded template using a DNA primer. Another T7 RNAP mutant having only the single substitution S641A loses RNA polymerase activity but is able to synthesize DNA.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(95)00732-O