Search Results - "Ly, Mellisa"

The proteoglycan bikunin has a defined sequence by Ly, Mellisa, Leach, Franklin E, Laremore, Tatiana N, Toida, Toshihiko, Amster, I Jonathan, Linhardt, Robert J

“…Complex polysaccharides are generally thought not to have a defined carbohydrate sequence because their synthesis is not template-directed. Detailed mass…”
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Hexuronic Acid Stereochemistry Determination in Chondroitin Sulfate Glycosaminoglycan Oligosaccharides by Electron Detachment Dissociation by Leach, Franklin E., Ly, Mellisa, Laremore, Tatiana N., Wolff, Jeremy J., Perlow, Jacob, Linhardt, Robert J., Amster, I. Jonathan

“…Electron detachment dissociation (EDD) has previously provided stereo-specific product ions that allow for the assignment of the acidic C-5stereochemistry in…”
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Analysis of E. coli K5 capsular polysaccharide heparosan by Ly, Mellisa, Wang, Zhenyu, Laremore, Tatiana N, Zhang, Fuming, Zhong, Weihong, Pu, Dennis, Zagorevski, Dmitri V, Dordick, Jonathan S, Linhardt, Robert J

“…Heparosan is the key precursor for the preparation of bioengineered heparin, a potential replacement for porcine intestinal heparin, an important anticoagulant…”
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Signal Amplification by Glyco-qPCR for Ultrasensitive Detection of Carbohydrates: Applications in Glycobiology by Kwon, Seok Joon, Lee, Kyung Bok, Solakyildirim, Kemal, Masuko, Sayaka, Ly, Mellisa, Zhang, Fuming, Li, Lingyun, Dordick, Jonathan S., Linhardt, Robert J.

“…Tiny amounts of carbohydrates (ca. 1 zmol) can be detected quantitatively by a real‐time method based on the conjugation of carbohydrates with DNA markers (see…”
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High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis by Laremore, Tatiana N., Ly, Mellisa, Solakyildirim, Kemal, Zagorevski, Dmitri V., Linhardt, Robert J.

“…Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6h using continuous elution…”
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Controlled photochemical depolymerization of K5 heparosan, a bioengineered heparin precursor by Higashi, Kyohei, Ly, Mellisa, Wang, Zhenyu, Masuko, Sayaka, Bhaskar, Ujjwal, Sterner, Eric, Zhang, Fuming, Toida, Toshihiko, Dordick, Jonathan S., Linhardt, Robert J.

“…• Heparosan produced from Escherichia coli is a precursor of bioengineered heparin. • Control of heparosan molecular weight is critical to produce a generic…”
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Domain structure elucidation of human decorin glycosaminoglycans by Laremore, Tatiana N, Ly, Mellisa, Zhang, Zhenqing, Solakyildirim, Kemal, McCallum, Scott A, Owens, Richard T, Linhardt, Robert J

“…The structure of the GAG (glycosaminoglycan) chain of recombinantly expressed decorin proteoglycan was examined using a combination of intact-chain analysis…”
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Proteoglycan sequence by Li, Lingyun, Ly, Mellisa, Linhardt, Robert J

“…Proteoglycans (PGs) are among the most structurally complex biomacromolecules in nature. They are present in all animal cells and frequently exert their…”
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Development of an activated carbon filtration step and high throughput screening method to remove host cell proteins from a recombinant enzyme process by Slocum, Ashley, Santora, Steven, Ly, Mellisa, Zhang, Junyan, Castano, Juan, Becerra‐Arteaga, Alejandro

“…An increasing number of non‐mAb recombinant proteins are being developed today. These biotherapeutics provide greater purification challenges where multiple…”
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Complete Mass Spectral Characterization of a Synthetic Ultralow-Molecular-Weight Heparin Using Collision-Induced Dissociation by Kailemia, Muchena J, Li, Lingyun, Ly, Mellisa, Linhardt, Robert J, Amster, I. Jonathan

“…Glycosaminoglycans (GAGs) are a class of biologically important molecules, and their structural analysis is the target of considerable research effort…”
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E. coli K5 fermentation and the preparation of heparosan, a bioengineered heparin precursor by Wang, Zhenyu, Ly, Mellisa, Zhang, Fuming, Zhong, Weihong, Suen, Amy, Hickey, Anne Marie, Dordick, Jonathan S, Linhardt, Robert J

“…Heparosan is an acidic polysaccharide natural product, which serves as the critical precursor in heparin biosynthesis and in the chemoenzymatic synthesis of…”
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Ultrasensitive Detection and Quantification of Acidic Disaccharides Using Capillary Electrophoresis and Quantum Dot-Based Fluorescence Resonance Energy Transfer by Chang, Yuqing, Cai, Chao, Li, Lingyun, Miao, Jianjun, Ucakturk, Ebru, Li, Guoyun, Ly, Mellisa, Linhardt, Robert J

“…Rapid and highly sensitive detection of the carbohydrate components of glycoconjugates is critical for advancing glycobiology. Fluorescence (or Förster)…”
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Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease by Zhong, Xiaotian, Jagarlapudi, Srinath, Weng, Yan, Ly, Mellisa, Rouse, Jason C., McClure, Kim, Ishino, Tetsuya, Zhang, Yan, Sousa, Eric, Cohen, Justin, Tzvetkova, Boriana, Cote, Kaffa, Scarcelli, John J., Johnson, Keith, Palandra, Joe, Apgar, James R., Yaddanapudi, Suma, Gonzalez-Villalobos, Romer A., Opsahl, Alan C., Lam, Khetemenee, Yao, Qing, Duan, Weili, Sievers, Annette, Zhou, Jing, Ferguson, Darren, D'Antona, Aaron, Zollner, Richard, Zhu, Hongli L., Kriz, Ron, Lin, Laura, Clerin, Valerie

“…The fortuitously discovered antiaging membrane protein αKlotho (Klotho) is highly expressed in the kidney, and deletion of the Klotho gene in mice causes a…”
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Proteoglycomics: recent progress and future challenges by Ly, Mellisa, Laremore, Tatiana N, Linhardt, Robert J

“…Proteoglycomics is a systematic study of structure, expression, and function of proteoglycans, a posttranslationally modified subset of a proteome. Although…”
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Control of the heparosan N-deacetylation leads to an improved bioengineered heparin by Wang, Zhenyu, Yang, Bo, Zhang, Zhenqing, Ly, Mellisa, Takieddin, Majde, Mousa, Shaker, Liu, Jian, Dordick, Jonathan S., Linhardt, Robert J.

“…The production of the anticoagulant drug heparin from non-animal sources has a number of advantages over the current commercial production of heparin. These…”
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Structural characterization of heparins from different commercial sources by Zhang, Fuming, Yang, Bo, Ly, Mellisa, Solakyildirim, Kemal, Xiao, Zhongping, Wang, Zhenyu, Beaudet, Julie M., Torelli, Amanda Y., Dordick, Jonathan S., Linhardt, Robert J.

“…Seven commercial heparin active pharmaceutical ingredients and one commercial low molecular weight from different manufacturers were characterized with a view…”
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Evolution of a comprehensive, orthogonal approach to sequence variant analysis for biotherapeutics by Lin, T. Jennifer, Beal, Kathryn M., Brown, Paul W., DeGruttola, Heather S., Ly, Mellisa, Wang, Wenge, Chu, Chia H., Dufield, Robert L., Casperson, Gerald F., Carroll, James A., Friese, Olga V., Figueroa, Bruno, Marzilli, Lisa A., Anderson, Karin, Rouse, Jason C.

“…Amino acid sequence variation in protein therapeutics requires close monitoring during cell line and cell culture process development. A cross-functional team…”
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Heparin Mapping Using Heparin Lyases and the Generation of a Novel Low Molecular Weight Heparin by Xiao, Zhongping, Tappen, Britney R, Ly, Mellisa, Zhao, Wenjing, Canova, Lauren P, Guan, Huashi, Linhardt, Robert J

“…Seven pharmaceutical heparins were investigated by oligosaccharide mapping by digestion with heparin lyase 1, 2, or 3, followed by high performance liquid…”
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Glycan Quantitation Strategies Based on High Sensitivity NanoLC/MS by Staples, G., Yin, H., Killeen, K., Ly, Mellisa

“…Monoclonal antibodies (mAb), therapeutic proteins for treatments of cancers and immunological diseases, need detailed structural elucidation and improved…”
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Stereoselective Synthesis of a C-Linked Neuraminic Acid Disaccharide: Potential Building Block for the Synthesis of C-Analogues of Polysialic acids by Kim, Jin-Hwan, Huang, Fei, Ly, Mellisa, Linhardt, Robert J

“…C-Linked neuraminic acid disaccharide was synthesized in a diastereoselective manner from a sulfone donor and aldehyde acceptor, which was protected as a…”
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