Search Results - "Lutje Spelberg, Jeffrey H"

Catalytic Promiscuity of Halohydrin Dehalogenase and its Application in Enantioselective Epoxide Ring Opening by Hasnaoui-Dijoux, Ghannia, Majerić Elenkov, Maja, Lutje Spelberg, Jeffrey H, Hauer, Bernhard, Janssen, Dick B

“…Easy virtue: Halohydrin dehalogenase is a highly promiscuous enzyme that can catalyze enantioselective epoxide ring opening with at least nine different…”
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Discovery of a thermostable Baeyer–Villiger monooxygenase by genome mining by Fraaije, Marco W, Wu, Jin, Heuts, Dominic P. H. M, van Hellemond, Erik W, Spelberg, Jeffrey H. Lutje, Janssen, Dick B

“…Baeyer–Villiger monooxygenases represent useful biocatalytic tools, as they can catalyze reactions which are difficult to achieve using chemical means…”
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Characterization of the Gene Cluster Involved in Isoprene Metabolism in Rhodococcus sp. Strain AD45 by van Hylckama Vlieg, J E, Leemhuis, H, Spelberg, J H, Janssen, D B

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Stereo-Complementary Two-Step Cascades Using a Two-Enzyme System Leading to Enantiopure Epoxides by Seisser, Birgit, Lavandera, Iván, Faber, Kurt, Spelberg, Jeffrey H. Lutje, Kroutil, Wolfgang

“…A novel one‐pot, two‐step, two‐enzyme cascade is described. Pro‐chiral α‐chloro ketones are stereoselectively reduced to the corresponding halohydrins as an…”
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Improved enantioselective conversion of styrene epoxides and meso-epoxides through epoxide hydrolases with a mutated nucleophile-flanking residue by van Loo, Bert, Kingma, Jaap, Heyman, Gertjan, Wittenaar, Alex, Lutje Spelberg, Jeffrey H., Sonke, Theo, Janssen, Dick B.

“…In epoxide hydrolase from Agrobacterium radiobacter (EchA), phenylalanine 108 flanks the nucleophilic aspartate and forms part of the substrate-binding pocket…”
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Halohydrin Dehalogenases Are Structurally and Mechanistically Related to Short-Chain Dehydrogenases/Reductases by van Hylckama Vlieg, J E, Tang, L, Lutje Spelberg, J H, Smilda, T, Poelarends, G J, Bosma, T, van Merode, A E, Fraaije, M W, Janssen, D B

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Highly Enantioselective and Regioselective Biocatalytic Azidolysis of Aromatic Epoxides by Lutje Spelberg, Jeffrey H, van Hylckama Vlieg, Johan E. T, Tang, Lixia, Janssen, Dick B, Kellogg, Richard M

“…The halohydrin dehalogenase from Agrobacterium radiobacter AD1 catalyzed the highly enantioselective and β-regioselective azidolysis of (substituted) styrene…”
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Exploration of the biocatalytic potential of a halohydrin dehalogenase using chromogenic substrates by Lutje Spelberg, Jeffrey H, Tang, Lixia, van Gelder, Marc, Kellogg, Richard M, Janssen, Dick B

“…Halohydrin dehalogenases are bacterial enzymes that catalyse the reversible formation of epoxides from vicinal halohydrins. A spectrophotometric assay for…”
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Steady-State Kinetics and Tryptophan Fluorescence Properties of Halohydrin Dehalogenase from Agrobacterium radiobacter. Roles of W139 and W249 in the Active Site and Halide-Induced Conformational Change by Tang, Lixia, van Merode, Annet E. J., Lutje Spelberg, Jeffrey H., Fraaije, Marco W., Janssen, Dick B.

“…Halohydrin dehalogenase (HheC) from Agrobacterium radiobacter AD1 is a homotetrameric protein containing four tryptophan residues per subunit. The fluorescence…”
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Tyrosine Residues Serve as Proton Donor in the Catalytic Mechanism of Epoxide Hydrolase from Agrobacterium radiobacter by Rink, Rick, Kingma, Jaap, Lutje Spelberg, Jeffrey H, Janssen, Dick B

“…Epoxide hydrolase from Agrobacterium radiobacter catalyzes the hydrolysis of epoxides to their diols via an alkyl-enzyme intermediate. The recently solved…”
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Mutation of Tyrosine Residues Involved in the Alkylation Half Reaction of Epoxide Hydrolase from Agrobacterium radiobacter AD1 Results in Improved Enantioselectivity by Rink, Rick, Lutje Spelberg, Jeffrey H, Pieters, Roland J, Kingma, Jaap, Nardini, Marco, Kellogg, Richard M, Dijkstra, Bauke W, Janssen, Dick B

“…Enantiomerically pure epoxides (oxiranes) are uniquely suited building blocks for synthetic purposes. Such epoxides are often prepared by means of remarkably…”
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Shifting the equilibrium of a biocatalytic cascade synthesis to enantiopure epoxides using anion exchangers by Schrittwieser, Joerg H., Lavandera, Iván, Seisser, Birgit, Mautner, Barbara, Lutje Spelberg, Jeffrey H., Kroutil, Wolfgang

“…Hydroxide-loaded anion exchangers have been successfully employed to shift the equilibrium of a one-pot, two-step, two-enzyme cascade reaction affording…”
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Enzymatic dynamic kinetic resolution of epihalohydrins by Lutje Spelberg, Jeffrey H, Tang, Lixia, Kellogg, Richard M, Janssen, Dick B

“…Graphic The haloalcohol dehalogenase from Agrobacterium radiobacter AD1 catalyses the reversible ring closure of vicinal haloalcohols to produce epoxides and…”
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Kinetic Mechanism and Enantioselectivity of Halohydrin Dehalogenase from Agrobacterium radiobacter by Tang, Lixia, Lutje Spelberg, Jeffrey H, Fraaije, Marco W, Janssen, Dick B

“…Halohydrin dehalogenase (HheC) from Agrobacterium radiobacter AD1 catalyzes the reversible intramolecular nucleophilic displacement of a halogen by a hydroxyl…”
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Biocatalytic Potential of the Epoxide Hydrolase from Agrobacterium radiobacter AD1 and a Mutant with Enhanced Enantioselectivity by Spelberg, Jeffrey H. Lutje, Rink, Rick, Archelas, Alain, Furstoss, Roland, Janssen, Dick B.

“…Optically pure epoxides are useful synthons for a variety of biologically active compounds. The epoxide hydrolase obtained from Agrobacterium radiobacter AD1…”
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A tandem enzyme reaction to produce optically active halohydrins, epoxides and diols by Lutje Spelberg, Jeffrey H., van Hylckama Vlieg, Johan E.T., Bosma, Tjibbe, Kellogg, Richard M., Janssen, Dick B.

“…The recombinant halohydrin dehalogenase from Agrobacterium radiobacter AD1 was used to obtain enantiomerically pure halohydrins and epoxides by kinetic…”
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Improved stability of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by replacement of cysteine residues by Tang, Lixia, van Hylckama Vlieg, Johan E.T, Lutje Spelberg, Jeffrey H, Fraaije, Marco W, Janssen, Dick B

“…Halohydrin dehalogenase from Agrobacterium radiobacter AD1 is a homo-tetrameric protein containing three cysteines per 28 kDa subunit. Under oxidizing…”
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Microbiological transformations. Part 48: Enantioselective biohydrolysis of 2-, 3- and 4-pyridyloxirane at high substrate concentration using the Agrobacterium radiobacter AD1 epoxide hydrolase and its Tyr215Phe mutant by Genzel, Yvonne, Archelas, Alain, Lutje Spelberg, Jeffrey H, Janssen, Dick B, Furstoss, Roland

“…The epoxide hydrolase (EH) from Agrobacterium radiobacter AD1 wild type (ArWT) and its Tyr215Phe mutant were compared for the biocatalyzed hydrolytic kinetic…”
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The enantioselectivity of haloalkane dehalogenases by Pieters, Roland J, Lutje Spelberg, Jeffrey H, Kellogg, Richard M, Janssen, Dick B

“…Two haloalkane dehalogenases were tested for their ability to perform kinetic resolutions of a series of racemic substrates and to convert meso substrates…”
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Nitrite-mediated hydrolysis of epoxides catalyzed by halohydrin dehalogenase from Agrobacterium radiobacter AD1: a new tool for the kinetic resolution of epoxides by Hasnaoui, Ghannia, Lutje Spelberg, Jeffrey H., de Vries, Erik, Tang, Lixia, Hauer, Bernhard, Janssen, Dick B.

“…[Display omitted] Halohydrin dehalogenase obtained from Agrobacterium radiobacter AD1, has been tested for the nitrite-mediated ring opening of epoxides. This…”
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