Search Results - "Lustbader, J. W"

Crystal structure of human chorionic gonadotropin by Lapthorn, A. J, Harris, D. C, Littlejohn, A, Lustbader, J. W, Canfield, R. E, Machin, K. J, Morgan, F. J, Isaacs, N. W

“…The three-dimensional structure of human chorionic gonadotropin shows that each of its two different subunits has a similar topology, with three disulphide…”
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Long-Acting Follicle-Stimulating Hormone Analogs Containing N-Linked Glycosylation Exhibited Increased Bioactivity Compared with O-Linked Analogs in Female Rats by Weenen, C., Peña, J. E., Pollak, S. V., Klein, J., Lobel, L., Trousdale, R. K., Palmer, S., Lustbader, E. G., Ogden, R. T., Lustbader, J. W.

“…The effects of altering the number and type of additional carbohydrate moieties on the pharmacokinetic and pharmacodynamic properties of FSH were examined in…”
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Development and characterization of a long‐acting recombinant hFSH agonist by Klein, J., Lobel, L., Pollak, S., Lustbader, B., Ogden, R.T., Sauer, M.V., Lustbader, J.W.

“…BACKGROUND: Fusion of the carboxyterminal peptide (CTP) of hCG to FSH results in a follitropin agonist with an extended half‐life, presumably due to the four…”
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ABAD Directly Links Aβ to Mitochondrial Toxicity in Alzheimer's Disease by Lustbader, Joyce W., Cirilli, Maurizio, Lin, Chang, Xu, Hong Wei, Takuma, Kazuhiro, Wang, Ning, Caspersen, Casper, Chen, Xi, Pollak, Susan, Chaney, Michael, Trinchese, Fabrizio, Liu, Shumin, Gunn-Moore, Frank, Lue, Lih-Fen, Walker, Douglas G., Kuppusamy, Periannan, Zewier, Zay L., Arancio, Ottavio, Stern, David, Yan, Shirley ShiDu, Wu, Hao

“…Mitochondrial dysfunction is a hallmark of β-amyloid (Aβ)-induced neuronal toxicity in Alzheimer's disease (AD). Here, we demonstrate that Aβ-binding alcohol…”
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A multifunctional prokaryotic protein expression system: overproduction, affinity purification, and selective detection by Kroll, D J, Abdel-Malek Abdel-Hafiz, H, Marcell, T, Simpson, S, Chen, C Y, Gutierrez-Hartmann, A, Lustbader, J W, Hoeffler, J P

“…A series of plasmid vectors, pRSET A, B, and C, have been developed for high-level protein expression in prokaryotes and have been characterized. Based upon…”
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Separation of nicked human chorionic gonadotropin (hCG), intact hCG, and hCG beta fragment from standard reference preparations and raw urine samples by Birken, S, Chen, Y, Gawinowicz, M A, Lustbader, J W, Pollak, S, Agosto, G, Buck, R, O'Connor, J

“…hCG is found in pregnancy urine and in urine from some cancer patients in a variety of forms whose concentrations have clinical importance. Recently, concerns…”
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Carbohydrate and peptide structure of the alpha- and beta-subunits of human chorionic gonadotropin from normal and aberrant pregnancy and choriocarcinoma by Elliott, M M, Kardana, A, Lustbader, J W, Cole, L A

“…Human chorionic gonadotropin (hCG), purified from the urine of 14 individuals with normal pregnancy, diabetic pregnancy, hydatidiform mole, or choriocarcinoma,…”
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Three-dimensional structures of gonadotropins by Lustbader, J.W., Pollak, S., Lobel, L., Trakht, I., Homans, S., Brown, J.M., Canfield, R.E.

“…Most secreted proteins are modified post-translationally with the addition of carbohydrate. It has been difficult to use crystallography to solve the…”
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Crystallization and characterization of human chorionic gonadotropin in chemically deglycosylated and enzymatically desialylated states by LUSTBADER, J. W, BIRKEN, S, PILEGGI, N. F, GAWINOWICZ KOLKS, M. A, POLLAK, S, CUFF, M. E, WEI YANG, HENDRICKSON, W. A, CANFIELD, R. E

“…Crystals suitable for X-ray diffraction studies at moderate resolution have been grown from two forms of human chorionic gonadotropin (hCG): HF-treated hCG and…”
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Hemoglobin-binding site on haptoglobin probed by selective proteolysis by Lustbader, J W, Arcoleo, J P, Birken, S, Greer, J

“…Selective proteolysis has been used to delineate the hemoglobin-binding site on haptoglobin heavy chain. Haptoglobin was cleaved specifically by plasmin,…”
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Preparation and characterization of antibodies to the urinary fragment of the human chorionic gonadotropin beta-subunit by Krichevsky, A, Armstrong, E G, Schlatterer, J, Birken, S, O'Connor, J, Bikel, K, Silverberg, S, Lustbader, J W, Canfield, R E

“…hCG is a glycoprotein hormone composed of two dissimilar subunits (alpha and beta) and is normally synthesized by trophoblastic tissue. Its measurement by…”
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A method for assessing the reassembly of a multisubunit glycoprotein by western blotting by Lustbader, J W, Pollak, S

“…The Western blot procedure has been adapted to detect the reassembly of a two-subunit glycoprotein, urinary human chorionic gonadotropin (hCG), directly on the…”
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Identification of multiple nuclear factors that interact with cyclic adenosine 3',5'-monophosphate response element-binding protein and activating transcription factor-2 by protein-protein interactions by Hoeffler, J P, Lustbader, J W, Chen, C Y

“…Understanding the nature and importance of protein-protein interactions in the mechanisms of eukaryotic gene expression is essential to understanding the…”
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Characterization of a bioeffective monoclonal antibody against thyrotropin beta-subunit by Hill, B L, Lustbader, J W, Erlanger, B F

“…To describe a region of the TSH molecule participating in binding to receptor, a monoclonal antibody specific for a TSH epitope shared by beta-subunits of…”
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The application of chemical studies of human chorionic gonadotropin to visualize its three-dimensional structure by Lustbader, J W, Yarmush, D L, Birken, S, Puett, D, Canfield, R E

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High-level bacterial expression of a natively folded, soluble extracellular domain fusion protein of the human luteinizing hormone/chorionic gonadotropin receptor in the cytoplasm of Escherichia coli by Lobel, L I, Pollak, S, Klein, J, Lustbader, J W

“…We have expressed the extracellular domain of the human luteinizing hormone/chorionic gonadotropin (hLH/CG) receptor as a fusion protein with thioredoxin in…”
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Expression of human chorionic gonadotropin uniformly labeled with NMR isotopes in Chinese hamster ovary cells: an advance toward rapid determination of glycoprotein structures by Lustbader, J W, Birken, S, Pollak, S, Pound, A, Chait, B T, Mirza, U A, Ramnarain, S, Canfield, R E, Brown, J M

“…Most secreted eukaryotic proteins are modified by glycosylation, and it has been difficult to solve their structures by crystallographic or NMR techniques…”
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Heterogeneity of proteins in commercial preparations of human chorionic gonadotropin (hCG) demonstrated by Western blotting by Morse, J H, Lustbader, J W, Harrington, J W, Canfield, R E

“…Immunoblotting has been utilized to detect the presence of human chorionic gonadotropin (hCG), its alpha and beta subunits, the Tamm-Horsfall protein…”
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Filamentous phage displaying the extracellular domain of the hLH/CG receptor bind hCG specifically by Lobel, L I, Rausch, P, Trakht, I, Pollak, S, Lustbader, J W

“…We have expressed the extracellular binding domain of the human LH/CG receptor as a fusion with the cpIII filamentous phage coat protein. These fusion phage…”
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Expression and characterization of recombinant beta-subunit hCG homodimer by Lobel, L, Pollak, S, Wang, S, Chaney, M, Lustbader, J W

“…We have linked two human chorionic gonadotropin (hCG) beta-subunit cDNAs in tandem such that the expressed fusion protein consists of two mature beta-subunits…”
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