Human Fibroblasts Produce Granulocyte-CSF, Macrophage-CSF, and Granulocyte-Macrophage-CSF Following Stimulation by Interleukin-1 and Poly(rI).Poly(rC)

Electrophoretically pure human interleukin-1 (IL-1) beta was found to stimulate human fibroblasts in a monolayer culture to elaborate colony-stimulating activity (CSA). Supernatant fluids from cultures induced with increasing concentrations of IL-1 were found to stimulate colony formation of myeloid...

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Bibliographic Details
Published in:	Blood Vol. 72; no. 3; pp. 860 - 866
Main Authors:	Fibbe, Willem E., Damme, Jo Van, Billiau, Alfons, Duinkerken, Nelleke, Lurvink, Elly, Ralph, Peter, Altrock, Bruce W., Kaushansky, Kenneth, Willemze, Roel, Falkenburg, J.H. Frederik
Format:	Journal Article
Language:	English
Published:	Washington, DC Elsevier Inc 01-09-1988 The Americain Society of Hematology
Subjects:	Analytical, structural and metabolic biochemistry Biological and medical sciences Colony-Forming Units Assay Colony-Stimulating Factors - biosynthesis Culture Media Fibroblasts - drug effects Fibroblasts - metabolism Fundamental and applied biological sciences. Psychology Granulocyte Colony-Stimulating Factor Granulocyte-Macrophage Colony-Stimulating Factor Growth Substances - biosynthesis Hematopoietic Stem Cells - drug effects Humans Interleukin-1 - pharmacology Kinetics Poly I-C - pharmacology Protein hormones. Growth factors. Cytokines Proteins Human Cell culture Induction Interleukin 1 Biosynthesis Colony stimulating factor Fibroblast Polyribonucleotide
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Summary:	Electrophoretically pure human interleukin-1 (IL-1) beta was found to stimulate human fibroblasts in a monolayer culture to elaborate colony-stimulating activity (CSA). Supernatant fluids from cultures induced with increasing concentrations of IL-1 were found to stimulate colony formation of myeloid (CFU-GM), erythroid (BFU-E), and multipotent (CFU-GEMM) progenitor cells in a dose-depen-dent fashion. The effect on mixed colony formation, however, was less than on CFU-GM and BFU-E growth. Similar to IL-1, the synthetical double-stranded RNA poly(rl).poly(rC) also stimulated release of CSA by fibroblasts. The kinetics of IL-1- and poly(rl).poly(rC)-induced CSA release were found to be different, in that poly(rl).poly(rC)-induced CSA production occurred more slowly. Anti-IL-1 antiserum was able to completely neutralize the IL-1 -induced CSA release, but had no effect on poly(rl).poly(rC)-induced CSF production, suggesting that the latter effect was mediated by other mechanisms than IL-1 in supernatant. By the use of specific immunologic assays, G-CSF, M-CSF, and GM-CSF could be identified in media conditioned by fibroblasts treated with IL-1 or poly(rl).poly(rC). Poly(rl).poly(rC) appeared to be a better inducer for M-CSF than IL-1.1988 by Grune & Stratton, Inc. 0006-4971/88/7203-0001$3.00/0.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0006-4971 1528-0020
DOI:	10.1182/blood.V72.3.860.860