Search Results - "Lunin, V G"
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Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models
Published in PloS one (28-04-2017)“…New innovative vaccines are highly needed to combat the global threat posed by tuberculosis. Efficient components-antigens and adjuvants-are crucial for…”
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Staphylococcus simulans recombinant lysostaphin: Production, purification, and determination of antistaphylococcal activity
Published in Biochemistry (Moscow) (01-05-2016)“…Staphylococcus simulans lysostaphin is an endopeptidase lysing staphylococcus cell walls by cleaving pentaglycine cross-bridges in their peptidoglycan. A…”
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Endonuclease Activity of MutL Protein of the Rhodobacter sphaeroides Mismatch Repair System
Published in Biochemistry (Moscow) (01-03-2018)“…We have purified the MutL protein from Rhodobacter sphaeroides mismatch repair system (rsMutL) for the first time. rsMutL demonstrated endonuclease activity in…”
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Chimeric Lactase Capable of Spontaneous and Strong Immobilization on Cellulose and Development of a Continuous-Flow System for Lactose Hydrolysis at High Temperatures
Published in Applied and Environmental Microbiology (01-12-2010)“…Recombinant plasmids containing fusion proteins composed of two different modules were constructed and expressed in Escherichia coli. The modules encoded the…”
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Regulation of the binding of the BMP-2 growth factor with collagen by blood plasma fibronectin
Published in Applied biochemistry and microbiology (01-03-2014)“…The release kinetics of recombinant human bone morphogenic factor 2 (rhBMP-2) from collagen hydrogel in the presence of human blood plasma have been studied…”
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Production of the recombinant human bone morphogenetic protein-2 in Escherichia coli and testing of its biological activity in vitro and in vivo
Published in Molecular biology (New York) (01-12-2010)“…Bone morphogenetic protein-2 (rhBMP-2) is an osteoinductive protein factor which plays a dominant role in growth and regeneration of bone tissue. In clinical…”
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Two variants of recombinant human bone morphogenetic protein-2 (rhBMP-2) with additional protein domains: Synthesis in an Escherichia coli heterologous expression system
Published in Biochemistry (Moscow) (01-05-2017)“…Two variants of recombinant human bone morphogenetic protein-2 (rhBMP-2) with additional N-terminal protein domains were obtained by expression in E. coli …”
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Recombinant RBD of the SARS-CoV-2 Spike Protein: Production in Escherichia coli Cells, Binding to Antibodies, and Antiviral Activity
Published in Molecular genetics, microbiology and virology (01-06-2023)“…The aim of the study is to synthesize a recombinant protein in Escherichia coli cells that carries the receptor-binding domain (RBD) of Spike protein of SARS…”
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Recombinant human erythropoietin with additional processable protein domains: Purification of protein synthesized in Escherichia coli heterologous expression system
Published in Biochemistry (Moscow) (01-11-2017)“…Three variants of human recombinant erythropoietin (rhEPO) with additional N-terminal protein domains were obtained by synthesis in an Escherichia coli…”
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Synthesis in Escherichia coli and Characterization of Human Recombinant Erythropoietin with Additional Heparin-Binding Domain
Published in Biochemistry (Moscow) (01-10-2018)“…Recombinant human erythropoietin (EPO) with additional N-terminal heparin-binding protein domain (HBD) from bone morphogenetic protein 2 was synthesized in…”
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Combined Effect of Bone Morphogenetic Protein-2 and Erythropoietin on Regeneration of Cranial Bone Defects in Mice
Published in Bulletin of experimental biology and medicine (01-07-2019)“…Using mouse model of regeneration of critical size cranial defects, we studied combined effect of 1 and 10 μg of BMP-2 of prokaryotic origin and recombinant…”
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Modern Approaches to Studies of New Osteogenic Biomaterials on the Model of Regeneration of Critical-Size Cranial Defects in Rats
Published in Bulletin of experimental biology and medicine (01-12-2016)“…Osteoinductive characteristics of new osteoplastic materials based on demineralized bone matrix of xenogenic origin with high and controlled degree of…”
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Isolation and characterization of collagen-binding domains from human von Willebrand factor
Published in Molecular genetics, microbiology and virology (01-09-2009)“…DNA fragments that encode two collagen-binding decapeptides from human von Willebrand factors vWF-H1 and vWF-H2 were cloned in Escherichia coli cells. The…”
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Disruption of bacterial biofilms using recombinant dispersin B
Published in Microbiology (New York) (01-07-2015)“…A synthetic gene encoding dispersin B of Aggregatibacter actinomycetemcomitans was cloned and expressed in Escherichia coli cells. Procedure for purification…”
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Vacuolar Na+/H+ antiporter from barley: identification and response to salt stress
Published in Biochemistry (Moscow) (01-01-2005)“…One of the protective mechanisms used by plants to survive under conditions of salt stress caused by high NaCl concentration is the removal of Na+ from the…”
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Recombinant Human Bone Morphogenetic Protein-2 (rhBMP-2) with Additional Protein Domain Synthesized in E. coli: In Vivo Osteoinductivity in Experimental Models on Small and Large Laboratory Animals
Published in Bulletin of experimental biology and medicine (01-12-2017)“…Recombinant human bone morphogenetic protein-2 with an additional s-tag domain (s-tag-BMP-2) synthesized in E. coli is characterized by higher solubility and…”
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Optimization of rhBMP-2 active-form production in a heterologous expression system using microbiological and molecular genetic approaches
Published in Molecular genetics, microbiology and virology (01-10-2016)“…Recombinant bone morphogenetic protein-2 (rhBMP-2) has pronounced osteoinductive properties, as evidenced by the results of experimental and clinical…”
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Influence of DNA aptamer structure on the specificity of binding to Taq DNA polymerase
Published in Biochemistry (Moscow) (01-02-2003)“…The secondary structure of DNA aptamer to Taq DNA polymerase was established as a hairpin. Both stem and loop structures of DNA ligand were shown to be…”
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Changed Serum Cytokine Profile in Mice in Response to Streptococcus A Culture
Published in Bulletin of experimental biology and medicine (01-05-2015)“…Comparative analysis of serum cytokine profiles of CBA mice was carried out 1, 5, 24, and 48 h after intraperitoneal injection of killed culture of different…”
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