Search Results - "Lukavsky, Peter"

Molecular basis of UG-rich RNA recognition by the human splicing factor TDP-43 by Lukavsky, Peter J, Daujotyte, Dalia, Tollervey, James R, Ule, Jernej, Stuani, Cristiana, Buratti, Emanuele, Baralle, Francisco E, Damberger, Fred F, Allain, Frédéric H-T

“…TDP-43 regulates alternative splicing of the cystic fibrosis transmembrane regulator (CFTR) and is found in cytosolic granules associated with several…”
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The Solution Structure of FUS Bound to RNA Reveals a Bipartite Mode of RNA Recognition with Both Sequence and Shape Specificity by Loughlin, Fionna E., Lukavsky, Peter J., Kazeeva, Tamara, Reber, Stefan, Hock, Eva-Maria, Colombo, Martino, Von Schroetter, Christine, Pauli, Phillip, Cléry, Antoine, Mühlemann, Oliver, Polymenidou, Magdalini, Ruepp, Marc-David, Allain, Frédéric H.-T.

“…Fused in sarcoma (FUS) is an RNA binding protein involved in regulating many aspects of RNA processing and linked to several neurodegenerative diseases…”
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Structure and function of HCV IRES domains by Lukavsky, Peter J.

“…The HCV IRES is a highly structured RNA which mediates cap-independent translation initiation in higher eukaryotes. This function is encoded in conserved…”
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NMR solution structure determination of large RNA-protein complexes by Yadav, Deepak Kumar, Lukavsky, Peter J

“…Structure determination of RNA-protein complexes is essential for our understanding of the multiple layers of RNA-mediated posttranscriptional regulation of…”
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Rapid, nondenaturing RNA purification using weak anion-exchange fast performance liquid chromatography by Easton, Laura E, Shibata, Yoko, Lukavsky, Peter J

“…We present a simple and fast method for large-scale purification of RNA oligonucleotides suitable for biochemical and structural studies. RNAs are transcribed…”
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Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition by Yadav, Deepak Kumar, Zigáčková, Dagmar, Zlobina, Maria, Klumpler, Tomáš, Beaumont, Christelle, Kubíčková, Monika, Vaňáčová, Štěpánka, Lukavsky, Peter J

“…Staufen1 (STAU1) is a dsRNA binding protein mediating mRNA transport and localization, translational control and STAU1-mediated mRNA decay (SMD). The STAU1…”
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A′-form RNA helices are required for cytoplasmic mRNA transport in Drosophila by Bullock, Simon L, Ringel, Inbal, Ish-Horowicz, David, Lukavsky, Peter J

“…The Drosophila fs(1)K10 transcript is localized asymmetrically during oogenesis through a cis -acting RNA element called the TLS. The tertiary structure of…”
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Cancer-Associated Substitutions in RNA Recognition Motifs of PUF60 and U2AF65 Reveal Residues Required for Correct Folding and 3′ Splice-Site Selection by Kralovicova, Jana, Borovska, Ivana, Kubickova, Monika, Lukavsky, Peter J., Vorechovsky, Igor

“…U2AF65 (U2AF2) and PUF60 (PUF60) are splicing factors important for recruitment of the U2 small nuclear ribonucleoprotein to lariat branch points and selection…”
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HCV and CSFV IRES domain II mediate eIF2 release during 80S ribosome assembly by Locker, Nicolas, Easton, Laura E, Lukavsky, Peter J

“…Internal ribosome entry site (IRES) RNAs from the hepatitis C virus (HCV) and classical swine fever virus (CSFV) coordinate cap‐independent assembly of…”
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The Indispensable N-Terminal Half of eIF3j/HCR1 Cooperates with its Structurally Conserved Binding Partner eIF3b/PRT1-RRM and with eIF1A in Stringent AUG Selection by ElAntak, Latifa, Wagner, Susan, Herrmannová, Anna, Karásková, Martina, Rutkai, Edit, Lukavsky, Peter J., Valášek, Leoš

“…Despite recent progress in our understanding of the numerous functions of individual subunits of eukaryotic translation initiation factor (eIF) 3, little is…”
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Bicaudal-D uses a parallel, homodimeric coiled coil with heterotypic registry to coordinate recruitment of cargos to dynein by Liu, Yang, Salter, Hannah K, Holding, Andrew N, Johnson, Christopher M, Stephens, Elaine, Lukavsky, Peter J, Walshaw, John, Bullock, Simon L

“…Cytoplasmic dynein is the major minus end-directed microtubule motor in eukaryotes. However, there is little structural insight into how different cargos are…”
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Efficient large-scale preparation and purification of short single-stranded RNA oligonucleotides by Zlobina, Maria, Sedo, Ondrej, Chou, Ming-Yuan, Slepankova, Lucia, Lukavsky, Peter J

“…Sequence-specific RNA recognition by RNA-binding proteins plays a crucial role in the post-translational regulation of gene expression. Biophysical and…”
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Conserved functional domains and a novel tertiary interaction near the pseudoknot drive translational activity of hepatitis C virus and hepatitis C virus-like internal ribosome entry sites by Easton, Laura E, Locker, Nicolas, Lukavsky, Peter J

“…The translational activity of the hepatitis C virus (HCV) internal ribosome entry site (IRES) and other HCV-like IRES RNAs depends on structured RNA elements…”
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Structure of HCV IRES domain II determined by NMR by Puglisi, Joseph D, Lukavsky, Peter J, Kim, Insil, Otto, Geoff A

“…Complex RNA structures regulate many biological processes, but are often too large for structure determination by NMR methods. The 5' untranslated region (5'…”
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Structural analysis of an eIF3 subcomplex reveals conserved interactions required for a stable and proper translation pre-initiation complex assembly by Herrmannová, Anna, Daujotyt, Dalia, Yang, Ji-Chun, Cuchalová, Lucie, Gorrec, Fabrice, Wagner, Susan, Dányi, István, Lukavsky, Peter J., Shivaya Valášek, Leoš

“…Translation initiation factor eIF3 acts as the key orchestrator of the canonical initiation pathway in eukaryotes, yet its structure is greatly unexplored. We…”
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Structure of eIF3b RNA recognition motif and its interaction with eIF3j: structural insights into the recruitment of eIF3b to the 40 S ribosomal subunit by ElAntak, Latifa, Tzakos, Andreas G, Locker, Nicolas, Lukavsky, Peter J

“…Mammalian eIF3 is a 700-kDa multiprotein complex essential for initiation of protein synthesis in eukaryotic cells. It consists of 13 subunits (eIF3a to -m),…”
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Large-scale preparation and purification of polyacrylamide-free RNA oligonucleotides by Lukavsky, Peter J, Puglisi, Joseph D

“…We present a fast and simple protocol for large-scale preparation and purification of RNA oligonucleotides. RNA oligonucleotides are prepared by in vitro…”
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Physicochemical analysis of rotavirus segment 11 supports a ‘modified panhandle’ structure and not the predicted alternative tRNA-like structure (TRLS) by Biswas, Subhajit, Li, Wilson, Manktelow, Emily, Lever, Jonathan, Easton, Laura E, Lukavsky, Peter J, Desselberger, Ulrich, Lever, Andrew M

“…Rotaviruses are a major cause of acute gastroenteritis, which is often fatal in infants. The viral genome consists of 11 double-stranded RNA segments, but…”
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Preparation of large RNA oligonucleotides with complementary isotope-labeled segments for NMR structural studies by Lukavsky, Peter J, Tzakos, Andreas G, Easton, Laura E

“…RNA structure determination by solution NMR spectroscopy is often restricted to small RNAs (<15 kDa) owing to the problem of chemical shift degeneracy. A…”
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NMR Study of 100 kDa HCV IRES RNA Using Segmental Isotope Labeling by Kim, Insil, Lukavsky, Peter J, Puglisi, Joseph D

“…RNA NMR is hindered by the large size of most biological RNAs. We present here a simple method for segmental isotopic labeling of an RNA fragment within the…”
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