Anti‐Zika Virus Activity of Plant Extracts Containing Polyphenols and Triterpenes on Vero CCL‐81 and Human Neuroblastoma SH‐SY5Y Cells

Anti‐Zika Virus Activity of Plant Extracts Containing Polyphenols and Triterpenes on Vero CCL‐81 and Human Neuroblastoma SH‐SY5Y Cells

Zika virus (ZIKV) infection is a global threat associated to neurological disorders in adults and microcephaly in children born to infected mothers. No vaccine or drug is available against ZIKV. We herein report the anti‐ZIKV activity of 36 plant extracts containing polyphenols and/or triterpenes. Z...

Full description

Saved in:
Bibliographic Details
Published in:Chemistry & biodiversity Vol. 19; no. 4; pp. e202100842 - n/a
Main Authors: Santos Pereira, Rosângela, Vasconcelos Costa, Vivian, Luiz Menezes Gomes, Gabriel, Rodrigues Valadares Campana, Priscilla, Maia de Pádua, Rodrigo, Barbosa, Milton, Oki, Yumi, Heiden, Gustavo, Fernandes, Geraldo Wilson, Menezes de Oliveira, Djalma, Souza, Daniele G., Martins Teixeira, Mauro, Castro Braga, Fernão
Format: Journal Article
Language:English
Published: Switzerland Wiley Subscription Services, Inc 01-04-2022
Subjects:
Animals
chemosystematic approach
Child
Chlorocebus aethiops
Cytotoxicity
Flavonoids
Humans
Maytenus
Maytenus ilicifolia
Microencephaly
Neuroblastoma
Neuroblastoma - drug therapy
Neurological diseases
Phenolic acids
Phenols
Plant extracts
Plant Extracts - pharmacology
Plant Extracts - therapeutic use
Polyphenols
Polyphenols - pharmacology
SH-SY5Y cells
Tandem Mass Spectrometry
Terminalia phaeocarpa
Triterpenes
Triterpenes - pharmacology
Vaccines
Vector-borne diseases
Vero Cells
Viruses
Zika Virus
Zika Virus Infection - drug therapy
Zika virus inhibition
Zika virus inhibition
Maytenus ilicifolia
chemosystematic approach
SH-SY5Y cells
Terminalia phaeocarpa
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Zika virus (ZIKV) infection is a global threat associated to neurological disorders in adults and microcephaly in children born to infected mothers. No vaccine or drug is available against ZIKV. We herein report the anti‐ZIKV activity of 36 plant extracts containing polyphenols and/or triterpenes. ZIKV‐infected Vero CCL‐81 cells were treated with samples at non‐cytotoxic concentrations, determined by MTT and LDH assays. One third of the extracts elicited concentration‐dependent anti‐ZIKV effect, with viral loads reduction from 0.4 to 3.8 log units. The 12 active extracts were tested on ZIKV‐infected SH‐SY5Y cells and significant reductions of viral loads (in log units) were induced by Maytenus ilicifolia (4.5 log), Terminalia phaeocarpa (3.7 log), Maytenus rigida (1.7 log) and Echinodorus grandiflorus (1.7 log) extracts. Median cytotoxic concentration (CC50) of these extracts in Vero cells were higher than in SH‐SY5Y lineage. M. ilicifolia (IC50=16.8±10.3 μg/mL, SI=3.4) and T. phaeocarpa (IC50=22.0±6.8 μg/mL, SI=4.8) were the most active extracts. UPLC‐ESI‐MS/MS analysis of M. ilicifolia extract led to the identification of 7 triterpenes, of which lupeol and a mixture of friedelin/friedelinol showed no activity against ZIKV. The composition of T. phaeocarpa extract comprises phenolic acids, ellagitannins and flavonoids, as recently reported by us. In conclusion, the anti‐ZIKV activity of 12 plant extracts is here described for the first time and polyphenols and triterpenes were identified as the probable bioactive constituents of T. phaeocarpa and M. ilicifolia, respectively.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1612-1872
1612-1880
DOI:10.1002/cbdv.202100842