Search Results - "Luhrmann, R"

Spliceosomal UsnRNP biogenesis, structure and function by Will, Cindy L, Lührmann, Reinhard

“…Significant advances have been made in elucidating the biogenesis pathway and three-dimensional structure of the UsnRNPs, the building blocks of the…”
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Essential Role for the Tudor Domain of SMN in Spliceosomal U snRNP Assembly: Implications for Spinal Muscular Atrophy by Bühler, Dirk, Raker, Veronica, Lührmann, Reinhard, Fischer, Utz

“…Spinal muscular atrophy (SMA) is a neurodegenerative disease of spinal motor neurons caused by reduced levels of functional survival of motor neurons (SMN)…”
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Symmetrical dimethylation of arginine residues in spliceosomal Sm protein B/B′ and the Sm-like protein LSm4, and their interaction with the SMN protein by BRAHMS, HERO, MEHEUS, LYDIE, DE BRABANDERE, VERONIQUE, FISCHER, UTZ, LÜHRMANN, REINHARD

“…Arginine residues in RG-rich proteins are frequently dimethylated posttranslationally by protein arginine methyltransferases (PRMTs). The most common…”
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Crystal Structure of the Spliceosomal 15.5kD Protein Bound to a U4 snRNA Fragment by Vidovic, Ivan, Nottrott, Stephanie, Hartmuth, Klaus, Lührmann, Reinhard, Ficner, Ralf

“…We have determined the crystal structure of a spliceosomal RNP complex comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5′ stem-loop of U4…”
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The snRNP core assembly pathway: identification of stable core protein heteromeric complexes and an snRNP subcore particle in vitro by Raker, V. A., Plessel, G., Lührmann, R.

“…Stable association of the eight common Sm proteins with U1, U2, U4 or U5 snRNA to produce a spliceosomal snRNP core structure is required for snRNP biogenesis,…”
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The Sm Domain Is an Ancient RNA-Binding Motif with Oligo(U) Specificity by Achsel, Tilmann, Stark, Holger, Lührmann, Reinhard

“…Sm and Sm-like proteins are members of a family of small proteins that is widespread throughout eukaryotic kingdoms. These proteins form heteromers with one…”
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Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores by Segref, Alexandra, Sharma, Kishore, Doye, Valérie, Hellwig, Andrea, Huber, Jochen, Lührmann, Reinhard, Hurt, Ed

“…An essential cellular factor for nuclear mRNA export called Mex67p which has homologous proteins in human and Caenorhabditis elegans was identified through its…”
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Crystal Structures of Two Sm Protein Complexes and Their Implications for the Assembly of the Spliceosomal snRNPs by Kambach, Christian, Walke, Stefan, Young, Robert, Avis, Johanna M., de la Fortelle, Eric, Raker, Veronica A., Lührmann, Reinhard, Li, Jade, Nagai, Kiyoshi

“…The U1, U2, U4/U6, and U5 small nuclear ribonucleoprotein particles (snRNPs) involved in pre-mRNA splicing contain seven Sm proteins (B/B′, D1, D2, D3, E, F,…”
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The C-terminal RG Dipeptide Repeats of the Spliceosomal Sm Proteins D1 and D3 Contain Symmetrical Dimethylarginines, Which Form a Major B-cell Epitope for Anti-Sm Autoantibodies by Brahms, Hero, Raymackers, Jos, Union, Ann, de Keyser, Filip, Meheus, Lydie, Lührmann, Reinhard

“…The Sm proteins B/B′, D1, D2, D3, E, F, and G are components of the small nuclear ribonucleoproteins U1, U2, U4/U6, and U5 that are essential for the splicing…”
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Protein functions in pre-mRNA splicing by Will, Cindy L, Lührmann, Reinhard

“…Proteins have been implicated in an expanding variety of functions during pre-mRNA splicing. Molecular cloning has identified genes encoding spliceosomal…”
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Functional interaction of a novel 15.5kD [U4/U6.U5] tri-snRNP protein with the 5' stem-loop of U4 snRNA by Nottrott, S, Hartmuth, K, Fabrizio, P, Urlaub, H, Vidovic, I, Ficner, R, Lührmann, R

“…Activation of the spliceosome for splicing catalysis requires the dissociation of U4 snRNA from the U4/U6 snRNA duplex prior to the first step of splicing. We…”
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Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure by WATKINS, NICHOLAS J., GOTTSCHALK, ALEXANDER, NEUBAUER, GITTE, KASTNER, BERTHOLD, FABRIZIO, PATRIZIA, MANN, MATTHIAS, LÜHRMANN, REINHARD

“…The eukaryotic nucleolus contains a large number of small nucleolar RNAs (snoRNAs) that are involved in pre-ribosomal RNA (pre-rRNA) processing. The H…”
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Sm protein-Sm site RNA interactions within the inner ring of the spliceosomal snRNP core structure by Urlaub, Henning, Raker, Veronica A., Kostka, Susanne, Lührmann, Reinhard

“…Seven Sm proteins, E, F, G, D1, D2, D3 and B/B′, assemble in a stepwise manner onto the single‐stranded Sm site element (PuAU4–6GPu) of the U1, U2, U4 and U5…”
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Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP by Gottschalk, A, Neubauer, G, Banroques, J, Mann, M, Luhrmann, R, Fabrizio, P

“…The 25S [U4/U6·U5] tri‐snRNP (small nuclear ribonucleoprotein) is a central unit of the nuclear pre‐mRNA splicing machinery. The U4, U5 and U6 snRNAs undergo…”
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Spliceosomal U snRNP Core Assembly: Sm Proteins Assemble onto an Sm Site RNA Nonanucleotide in a Specific and Thermodynamically Stable Manner by Raker, Veronica A., Hartmuth, Klaus, Kastner, Berthold, Lührmann, Reinhard

“…Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Identification of Both Shared and Distinct Proteins in the Major and Minor Spliceosomes by Will, Cindy L., Schneider, Claudia, Reed, Robin, Lührmann, Reinhard

“…In metazoans, two distinct spliceosomes catalyzing pre-messenger RNA splicing have been identified. Here, the human U11/U12 small nuclear ribonucleoprotein…”
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An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2 by Fabrizio, Patrizia, Laggerbauer, Bernhard, Lauber, Jürgen, Lane, William S., Lührmann, Reinhard

“…The driving forces behind the many RNA conformational changes occurring in the spliceosome are not well understood. Here we characterize an evolutionarily…”
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High Intranuclear Mobility and Dynamic Clustering of the Splicing Factor U1 snRNP Observed by Single Particle Tracking by Kues, Thorsten, Dickmanns, Achim, Lührmann, Reinhard, Peters, Reiner, Kubitscheck, Ulrich

“…Uridine-rich small nuclear ribonucleoproteins (U snRNPs) are components of the splicing machinery that removes introns from precursor mRNA. Like other splicing…”
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A general approach for identification of RNA-protein cross-linking sites within native human spliceosomal small nuclear ribonucleoproteins (snRNPs). Analysis of RNA-protein contacts in native U1 and U4/U6.U5 snRNPs by Urlaub, H, Hartmuth, K, Kostka, S, Grelle, G, Lührmann, R

“…We describe a novel approach to identify RNA-protein cross-linking sites within native small nuclear ribonucleoprotein (snRNP) particles from HeLa cells. It…”
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A doughnut-shaped heteromer of human Sm-like proteins binds to the 3′-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro by Achsel, Tilmann, Brahms, Hero, Kastner, Berthold, Bachi, Angela, Wilm, Matthias, Lührmann, Reinhard

“…We describe the isolation and molecular characterization of seven distinct proteins present in human [U4/U6·U5] tri‐snRNPs. These proteins exhibit clear…”
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