Adaptation and validation of antibody-ELISA using dried blood spots on filter paper for epidemiological surveys of tsetse-transmitted trypanosomosis in cattle

The indirect enzyme-linked immunosorbent assay (ELISA) for the detection of anti-trypanosomal antibodies in bovine serum was adapted for use with dried blood spots on filter paper. Absorbance (450 nm) results for samples were expressed as percent positivity, i.e. percentage of the median absorbance...

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Bibliographic Details
Published in:	Preventive veterinary medicine Vol. 37; no. 1; pp. 91 - 99
Main Authors:	Hopkins, Julian S, Chitambo, Harrison, Machila, Noreen, Luckins, Anthony G, Rae, Phillip F, van den Bossche, Peter, Eisler, Mark C
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-12-1998
Subjects:	Animals ANTIBODIES Antibodies, Protozoan - blood Antibody ANTICORPS ANTICUERPOS BOVIN CATTLE Cattle parasitological diseases DIAGNOSIS DIAGNOSTIC DIAGNOSTICO DISEASE SURVEYS ELISA ENCUESTAS SANITARIAS ENQUETE PATHOLOGIQUE Enzyme-Linked Immunosorbent Assay - methods GANADO BOVINO Prevalence Reproducibility of Results Sensitivity Seroepidemiologic Studies Specificity TEST ELISA TRIPANOSOMIASIS TRYPANOSOMA BRUCEI TRYPANOSOMA CONGOLENSE TRYPANOSOMA VIVAX TRYPANOSOMIASIS Trypanosomiasis, African - veterinary Trypanosomiasis, Bovine - diagnosis Trypanosomiasis, Bovine - epidemiology Trypanosomiasis, Bovine - immunology TRYPANOSOMOSE Trypanosomosis ZAMBIA Zambia - epidemiology ZAMBIE Zambia Cattle parasitological diseases Sensitivity Specificity Antibody Diagnosis ELISA Trypanosomosis
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Summary:	The indirect enzyme-linked immunosorbent assay (ELISA) for the detection of anti-trypanosomal antibodies in bovine serum was adapted for use with dried blood spots on filter paper. Absorbance (450 nm) results for samples were expressed as percent positivity, i.e. percentage of the median absorbance result of four replicates of the strong positive control serum. The antibody-ELISA was evaluated in Zambia for use in epidemiological surveys of the prevalence of tsetse-transmitted bovine trypanosomosis. Known negative samples (sera, n=209; blood spots, n=466) were obtained from cattle from closed herds in tsetse-free areas close to Lusaka. Known positive samples (sera, n=367; blood spots, n=278) were obtained from cattle in Zambia's Central, Lusaka and Eastern Provinces, diagnosed as being infected with Trypanosoma brucei, T. congolense, or T. vivax using the phase-contrast buffy-coat technique or Giemsa-stained thick and thin blood smears. For sera (at a cut-off value of 23.0% positivity) sensitivity and specificity were 86.1 and 95.2%, respectively. For bloodspots (at a cut-off value of 18.8% positivity) sensitivity and specificity were 96.8 and 95.7%, respectively. The implications of persistence of antibodies following treatment or self-cure are discussed.
Bibliography:	1999002242 L72 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0167-5877 1873-1716
DOI:	10.1016/S0167-5877(98)00101-9