Antibacterial and Antimetastatic Potential of Diospyros lycioides Extract on Cervical Cancer Cells and Associated Pathogens

Antibacterial and Antimetastatic Potential of Diospyros lycioides Extract on Cervical Cancer Cells and Associated Pathogens

Cervical cancer is among the most prevalent forms of cancer in women worldwide. Diospyros lycioides was extracted using hexane, ethyl acetate, acetone, and methanol and finger print profiles were determined. The leaf material was tested for the presence of flavonoids, tannins, saponins, terpenoids,...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine Vol. 2016; no. 2016; pp. 1 - 10
Main Authors: Mampuru, Leseilane, Mokgotho, Matlou Phineas, Ndiitwani, T., Lubisi, V. Z., Bagla, V. P., Mbazima, Vusi
Format: Journal Article
Language:English
Published: Cairo, Egypt Hindawi Publishing Corporation 01-01-2016
John Wiley & Sons, Inc
Hindawi Limited
Subjects:
Acetone
Acids
Ammonia
Antibacterial agents
Antioxidants
Apoptosis
Breast cancer
Cancer cells
Cell adhesion & migration
Cell cycle
Cervical cancer
Chromatography
Colleges & universities
Enterococcus faecalis
Escherichia coli
Esters
Ethanol
Extracellular matrix
Flavonoids
Hydrocarbons
Infertility
Isoflavones
Medicine, Botanic
Medicine, Herbal
Metastasis
Pathogens
Pseudomonas aeruginosa
Saponins
Staphylococcus aureus
Womens health
South Africa
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Summary:Cervical cancer is among the most prevalent forms of cancer in women worldwide. Diospyros lycioides was extracted using hexane, ethyl acetate, acetone, and methanol and finger print profiles were determined. The leaf material was tested for the presence of flavonoids, tannins, saponins, terpenoids, and cardiac glycosides using standard chemical methods and the presence of flavonoids and phenolics using thin layer chromatography. The total phenolic content was determined using Folin-Ciocalteu procedure. The four extracts were tested for antibacterial activity using bioautography against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli. The acetone extract with the highest number of antibacterial and antioxidant compounds was assessed for its cytotoxicity on BUD-8 cells using the real-time xCELLigence system and its potential effects on metastatic cervical cancer (HeLa) cell migration and invasion were assessed using wound healing migration and invasion assays. The leaf extract tested positive for flavonoids, tannins, and terpenoids while the four different extracts tested in the antimicrobial assay contained constituents active against one or more of the organisms tested, except E. coli. The cytotoxicity of the acetone extract in real-time was concentration-dependent with potent ability to suppress the migration and invasion of HeLa cells. The finding demonstrates the acetone extract to contain constituents with antibacterial and antimetastatic effects on cervical cancer cells.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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Academic Editor: Daniela Rigano
ISSN:1741-427X
1741-4288
DOI:10.1155/2016/5342082