The virome of bubaline (Bubalus bubalis) tonsils reveals an unreported bubaline polyomavirus

Water buffalo ( Bubalus bubalis ) farming is increasing in many regions of the world due to the species’ ability to thrive in environments where bovine cattle would struggle. Despite water buffaloes being known for their resistance to diseases, there is a lack of data about the diversity of the micr...

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Published in:	Brazilian journal of microbiology Vol. 55; no. 3; pp. 2893 - 2900
Main Authors:	Timm, Francine C. B., Campos, Fabrício Souza, Janssen, Luis, dos Santos, Raíssa Nunes, Paredes-Galarza, Bruna, Stone, Nicole Vieira, Oliveira, Martha Trindade, Gasparetto, Raíssa, Müller, Nicolas Felipe Drum, Melgarejo, Alanis da Silva, Corrêa, Miguel Leonetti, Lozano, Lina Marcela Violet, Salvato, Richard Steiner, Godinho, Fernanda Marques de Souza, Barcellos, Regina Bones, Teixeira, Maria Audiléia da Silva, Riet-Correa, Gabriela, Cerqueira, Valíria Duarte, Bezerra Júnior, Pedro Soares, Franco, Ana Cláudia, Roehe, Paulo Michel
Format:	Journal Article
Language:	English
Published:	Cham Springer International Publishing 01-09-2024 Springer Nature B.V
Subjects:	Amplification Animals Biomedical and Life Sciences Brazil Bubalus bubalis Buffalo Buffaloes - virology Cattle Circular DNA Deoxyribonucleic acid DNA DNA polymerase DNA viruses DNA, Viral - genetics DNA-directed DNA polymerase Food Microbiology Gene sequencing Genera Genome, Viral Genomes Harbors Life Sciences Medical Microbiology Microbial Ecology Microbial Genetics and Genomics Microbiology Microbiomes Mycology Nucleotide sequence Palatine Tonsil - virology Phylogeny Polyomavirus - classification Polyomavirus - genetics Polyomavirus - isolation & purification Tonsil Veterinary and Animal Production - Research Paper Virome Brazil Tonsils Marajó Island, Brazil Virome Circular DNA viruses Water buffalo
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Summary:	Water buffalo ( Bubalus bubalis ) farming is increasing in many regions of the world due to the species’ ability to thrive in environments where bovine cattle would struggle. Despite water buffaloes being known for their resistance to diseases, there is a lack of data about the diversity of the microbiome of the species. In this study, we examined the virome diversity in palatine tonsils collected from animals from the island of Marajó, northern Pará state, Brazil, which harbors the largest bubaline flock in the country. Tonsil fragments from 60 clinically healthy bubalines were randomly selected from a sample of 293 animals. The samples were purified, extracted, and randomly amplified with phi29 DNA polymerase . After amplification, the products were purified and sequenced. Circular DNA viruses were predominant in the tonsils’ virome. Sequences of genome segments representative of members of the genera Alphapolyomavirus (including a previously unreported bubaline polyomavirus genome) and Gemycircularvirus were identified, along with other not yet classified circular virus genomes. As the animals were clinically healthy at the time of sampling, such viruses likely constitute part of the normal tonsillar virome of water buffaloes inhabiting the Ilha do Marajó biome.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1517-8382 1678-4405 1678-4405
DOI:	10.1007/s42770-024-01449-0