Search Results - "Lowden, Michael J."
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Oxford nanopore sequencing enables rapid discovery of single-domain antibodies from phage display libraries
Published in BioTechniques (01-12-2018)“…Antibody (Ab) repertoire sequencing using high-throughput massively parallel technologies has contributed substantially to the understanding of Ab responses…”
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Structure of Vibrio cholerae ToxT Reveals a Mechanism for Fatty Acid Regulation of Virulence Genes
Published in Proceedings of the National Academy of Sciences - PNAS (16-02-2010)“…Cholera is an acute intestinal infection caused by the bacterium Vibrio cholerae. In order for V. cholerae to cause disease, it must produce two virulence…”
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3
Isolation and characterization of camelid single-domain antibodies against HER2
Published in BMC research notes (05-12-2018)“…To isolate and characterize novel high-affinity llama single-domain antibodies against human HER2. We immunized a llama with human HER2, constructed a…”
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Stability-Diversity Tradeoffs Impose Fundamental Constraints on Selection of Synthetic Human VH/VL Single-Domain Antibodies from In Vitro Display Libraries
Published in Frontiers in immunology (12-12-2017)“…Human autonomous VH/VL single-domain antibodies (sdAbs) are attractive therapeutic molecules, but often suffer from suboptimal stability, solubility and…”
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5
Salmonella phage ST64B encodes a member of the SseK/NleB effector family
Published in PloS one (18-03-2011)“…Salmonella enterica is a species of bacteria that is a major cause of enteritis across the globe, while certain serovars cause typhoid, a more serious disease…”
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Pathogenic adaptation of intracellular bacteria by rewiring a cis-regulatory input function
Published in Proceedings of the National Academy of Sciences - PNAS (10-03-2009)“…The acquisition of DNA by horizontal gene transfer enables bacteria to adapt to previously unexploited ecological niches. Although horizontal gene transfer and…”
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Negative Regulation of Salmonella Pathogenicity Island 2 Is Required for Contextual Control of Virulence during Typhoid
Published in Proceedings of the National Academy of Sciences - PNAS (29-11-2005)“…Salmonella enterica relies on a type III secretion system encoded in Salmonella pathogenicity island-2 (SPI-2) to survive and replicate within macrophages at…”
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SseL Is a Salmonella-Specific Translocated Effector Integrated into the SsrB-Controlled Salmonella Pathogenicity Island 2 Type III Secretion System
Published in Infection and Immunity (01-02-2007)“…Bacterial pathogens use horizontal gene transfer to acquire virulence factors that influence host colonization, alter virulence traits, and ultimately shape…”
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A Highly Effective Component Vaccine against Nontyphoidal Salmonella enterica Infections
Published in mBio (22-09-2015)“…Nontyphoidal Salmonella enterica (NTS) infections are a major burden to global public health, as they lead to diseases ranging from gastroenteritis to systemic…”
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Incorporation of a Novel CD16-Specific Single-Domain Antibody into Multispecific Natural Killer Cell Engagers With Potent ADCC
Published in Molecular pharmaceutics (07-06-2021)“…Multispecific antibodies that bridge immune effector and tumor cells have shown promising preclinical and clinical efficacies. Here, we isolated and…”
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Sequence tolerance of immunoglobulin variable domain framework regions to noncanonical intradomain disulfide linkages
Published in The Journal of biological chemistry (01-11-2023)“…Most immunoglobulin (Ig) domains bear only a single highly conserved canonical intradomain, inter-β-sheet disulfide linkage formed between Cys23-Cys104, and…”
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Facile Affinity Maturation of Single-Domain Antibodies Using Next-Generation DNA Sequencing
Published in Methods in molecular biology (Clifton, N.J.) (2022)“…Binding affinity is one of the primary determinants of antibody function. Here, we provide a protocol for simple and rapid affinity maturation of single-domain…”
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The stress induced caleosin, RD20/CLO3, acts as a negative regulator of GPA1 in Arabidopsis
Published in Plant molecular biology (01-10-2021)“…Key message A stress induced calcium-binding protein, RD20/CLO3 interacts with the alpha subunit of the heterotrimeric G-protein complex in Arabidopsis and…”
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Carbohydrate esterase family 16 contains fungal hemicellulose acetyl esterases (HAEs) with varying specificity
Published in New biotechnology (25-09-2022)“…Acetyl esterases are an important component of the enzymatic machinery fungi use to degrade plant biomass and are classified in several Carbohydrate Esterase…”
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A disulfide-stabilized human VL single-domain antibody library is a source of soluble and highly thermostable binders
Published in Molecular immunology (01-10-2017)“…We have previously shown that incorporation of a second intradomain disulfide linkage into camelid VHH and human VH/VL single-domain antibodies confers…”
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A disulfide-stabilized human V L single-domain antibody library is a source of soluble and highly thermostable binders
Published in Molecular immunology (01-10-2017)“…We have previously shown that incorporation of a second intradomain disulfide linkage into camelid V H and human V /V single-domain antibodies confers…”
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Stability-Diversity Tradeoffs Impose Fundamental Constraints on Selection of Synthetic Human V H /V L Single-Domain Antibodies from In Vitro Display Libraries
Published in Frontiers in immunology (12-12-2017)“…Human autonomous V /V single-domain antibodies (sdAbs) are attractive therapeutic molecules, but often suffer from suboptimal stability, solubility and…”
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Quantitative Mass Spectrometry Catalogues Salmonella Pathogenicity Island-2 Effectors and Identifies Their Cognate Host Binding Partners
Published in The Journal of biological chemistry (08-07-2011)“…Gram-negative bacterial pathogens have developed specialized secretion systems to transfer bacterial proteins directly into host cells. These bacterial…”
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Functional and structural diversity in GH62 α‐L‐arabinofuranosidases from the thermophilic fungus Scytalidium thermophilum
Published in Microbial biotechnology (01-05-2015)“…Summary The genome of the thermophilic fungus Scytalidium thermophilum (strain CBS 625.91) harbours a wide range of genes involved in carbohydrate degradation,…”
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Structural functional analysis of Abf62C and Abf62A
Published in Microbial biotechnology (01-05-2015)“…The genome of the thermophilic fungus Scytalidium thermophilum (strain CBS 625.91) harbours a wide range of genes involved in carbohydrate degradation,…”
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