Search Results - "Lowden, Michael J."

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  1. 1

    Oxford nanopore sequencing enables rapid discovery of single-domain antibodies from phage display libraries by Lowden, Michael J, Henry, Kevin A

    Published in BioTechniques (01-12-2018)
    “…Antibody (Ab) repertoire sequencing using high-throughput massively parallel technologies has contributed substantially to the understanding of Ab responses…”
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    Journal Article
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    Structure of Vibrio cholerae ToxT Reveals a Mechanism for Fatty Acid Regulation of Virulence Genes by Lowden, Michael J., Skorupski, Karen, Pellegrini, Maria, Chiorazzo, Michael G., Taylor, Ronald K., Kull, F. Jon, Mekalanos, John J.

    “…Cholera is an acute intestinal infection caused by the bacterium Vibrio cholerae. In order for V. cholerae to cause disease, it must produce two virulence…”
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  3. 3

    Isolation and characterization of camelid single-domain antibodies against HER2 by Hussack, Greg, Raphael, Shalini, Lowden, Michael J, Henry, Kevin A

    Published in BMC research notes (05-12-2018)
    “…To isolate and characterize novel high-affinity llama single-domain antibodies against human HER2. We immunized a llama with human HER2, constructed a…”
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  4. 4

    Stability-Diversity Tradeoffs Impose Fundamental Constraints on Selection of Synthetic Human VH/VL Single-Domain Antibodies from In Vitro Display Libraries by Henry, Kevin A., Kim, Dae Young, Kandalaft, Hiba, Lowden, Michael J., Yang, Qingling, Schrag, Joseph D., Hussack, Greg, MacKenzie, C. Roger, Tanha, Jamshid

    Published in Frontiers in immunology (12-12-2017)
    “…Human autonomous VH/VL single-domain antibodies (sdAbs) are attractive therapeutic molecules, but often suffer from suboptimal stability, solubility and…”
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  5. 5

    Salmonella phage ST64B encodes a member of the SseK/NleB effector family by Brown, Nat F, Coombes, Brian K, Bishop, Jenna L, Wickham, Mark E, Lowden, Michael J, Gal-Mor, Ohad, Goode, David L, Boyle, Erin C, Sanderson, Kristy L, Finlay, B Brett

    Published in PloS one (18-03-2011)
    “…Salmonella enterica is a species of bacteria that is a major cause of enteritis across the globe, while certain serovars cause typhoid, a more serious disease…”
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    Pathogenic adaptation of intracellular bacteria by rewiring a cis-regulatory input function by Osborne, Suzanne E, Walthers, Don, Tomljenovic, Ana M, Mulder, David T, Silphaduang, Uma, Duong, Nancy, Lowden, Michael J, Wickham, Mark E, Waller, Ross F, Kenney, Linda J, Coombes, Brian K

    “…The acquisition of DNA by horizontal gene transfer enables bacteria to adapt to previously unexploited ecological niches. Although horizontal gene transfer and…”
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  7. 7

    Negative Regulation of Salmonella Pathogenicity Island 2 Is Required for Contextual Control of Virulence during Typhoid by Brian K. Coombes, Wickham, Mark E., Michael J. Lowden, Brown, Nat F., Finlay, B. Brett, Curtiss, Roy

    “…Salmonella enterica relies on a type III secretion system encoded in Salmonella pathogenicity island-2 (SPI-2) to survive and replicate within macrophages at…”
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  8. 8

    SseL Is a Salmonella-Specific Translocated Effector Integrated into the SsrB-Controlled Salmonella Pathogenicity Island 2 Type III Secretion System by Coombes, Brian K, Lowden, Michael J, Bishop, Jennifer L, Wickham, Mark E, Brown, Nat F, Duong, Nancy, Osborne, Suzanne, Gal-Mor, Ohad, Finlay, B. Brett

    Published in Infection and Immunity (01-02-2007)
    “…Bacterial pathogens use horizontal gene transfer to acquire virulence factors that influence host colonization, alter virulence traits, and ultimately shape…”
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    Incorporation of a Novel CD16-Specific Single-Domain Antibody into Multispecific Natural Killer Cell Engagers With Potent ADCC by van Faassen, Henk, Jo, Dong-Hyeon, Ryan, Shannon, Lowden, Michael J, Raphael, Shalini, MacKenzie, C. Roger, Lee, Seung-Hwan, Hussack, Greg, Henry, Kevin A

    Published in Molecular pharmaceutics (07-06-2021)
    “…Multispecific antibodies that bridge immune effector and tumor cells have shown promising preclinical and clinical efficacies. Here, we isolated and…”
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  11. 11

    Sequence tolerance of immunoglobulin variable domain framework regions to noncanonical intradomain disulfide linkages by Kim, Dae Young, Kandalaft, Hiba, Lowden, Michael J., Yang, Qingling, Rossotti, Martin A., Robotham, Anna, Kelly, John F., Hussack, Greg, Schrag, Joseph D., Henry, Kevin A., Tanha, Jamshid

    Published in The Journal of biological chemistry (01-11-2023)
    “…Most immunoglobulin (Ig) domains bear only a single highly conserved canonical intradomain, inter-β-sheet disulfide linkage formed between Cys23-Cys104, and…”
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  12. 12

    Facile Affinity Maturation of Single-Domain Antibodies Using Next-Generation DNA Sequencing by Lowden, Michael J, van Faassen, Henk, Raphael, Shalini, Ryan, Shannon, Hussack, Greg, Henry, Kevin A

    “…Binding affinity is one of the primary determinants of antibody function. Here, we provide a protocol for simple and rapid affinity maturation of single-domain…”
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  13. 13

    The stress induced caleosin, RD20/CLO3, acts as a negative regulator of GPA1 in Arabidopsis by Brunetti, Sabrina C., Arseneault, Michelle K. M., Wright, Justin A., Wang, Zhejun, Ehdaeivand, Mohammad-Reza, Lowden, Michael J., Rivoal, Jean, Khalil, Hala B., Garg, Gajra, Gulick, Patrick J.

    Published in Plant molecular biology (01-10-2021)
    “…Key message A stress induced calcium-binding protein, RD20/CLO3 interacts with the alpha subunit of the heterotrimeric G-protein complex in Arabidopsis and…”
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    A disulfide-stabilized human VL single-domain antibody library is a source of soluble and highly thermostable binders by Henry, Kevin A., Kandalaft, Hiba, Lowden, Michael J., Rossotti, Martin A., van Faassen, Henk, Hussack, Greg, Durocher, Yves, Kim, Dae Young, Tanha, Jamshid

    Published in Molecular immunology (01-10-2017)
    “…We have previously shown that incorporation of a second intradomain disulfide linkage into camelid VHH and human VH/VL single-domain antibodies confers…”
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  16. 16

    A disulfide-stabilized human V L single-domain antibody library is a source of soluble and highly thermostable binders by Henry, Kevin A, Kandalaft, Hiba, Lowden, Michael J, Rossotti, Martin A, van Faassen, Henk, Hussack, Greg, Durocher, Yves, Kim, Dae Young, Tanha, Jamshid

    Published in Molecular immunology (01-10-2017)
    “…We have previously shown that incorporation of a second intradomain disulfide linkage into camelid V H and human V /V single-domain antibodies confers…”
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  17. 17

    Stability-Diversity Tradeoffs Impose Fundamental Constraints on Selection of Synthetic Human V H /V L Single-Domain Antibodies from In Vitro Display Libraries by Henry, Kevin A, Kim, Dae Young, Kandalaft, Hiba, Lowden, Michael J, Yang, Qingling, Schrag, Joseph D, Hussack, Greg, MacKenzie, C Roger, Tanha, Jamshid

    Published in Frontiers in immunology (12-12-2017)
    “…Human autonomous V /V single-domain antibodies (sdAbs) are attractive therapeutic molecules, but often suffer from suboptimal stability, solubility and…”
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    Journal Article
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    Functional and structural diversity in GH62 α‐L‐arabinofuranosidases from the thermophilic fungus Scytalidium thermophilum by Kaur, Amrit Pal, Nocek, Boguslaw P., Xu, Xiaohui, Lowden, Michael J., Leyva, Juan Francisco, Stogios, Peter J., Cui, Hong, Di Leo, Rosa, Powlowski, Justin, Tsang, Adrian, Savchenko, Alexei

    Published in Microbial biotechnology (01-05-2015)
    “…Summary The genome of the thermophilic fungus Scytalidium thermophilum (strain CBS 625.91) harbours a wide range of genes involved in carbohydrate degradation,…”
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  20. 20

    Structural functional analysis of Abf62C and Abf62A by Amrit Pal Kaur, Nocek, Boguslaw P, Xu, Xiaohui, Lowden, Michael J, Leyva, Juan Francisco, Stogios, Peter J, Cui, Hong, Rosa Di Leo, Powlowski, Justin, Tsang, Adrian, Savchenko, Alexei

    Published in Microbial biotechnology (01-05-2015)
    “…The genome of the thermophilic fungus Scytalidium thermophilum (strain CBS 625.91) harbours a wide range of genes involved in carbohydrate degradation,…”
    Get full text
    Journal Article