Search Results - "Loughran, Sinead"

Purification of Polyhistidine-Tagged Proteins by Loughran, Sinéad T, Bree, Ronan T, Walls, Dermot

“…His-tagging is the most widespread and versatile strategy used to purify recombinant proteins for biochemical and structural studies. Recombinant DNA methods…”
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Tagging Recombinant Proteins to Enhance Solubility and Aid Purification by Loughran, Sinéad T, Walls, Dermot

“…Protein fusion technology has had a major impact on the efficient production and purification of individual recombinant proteins. The use of genetically…”
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Tagging recombinant proteins to enhance solubility and aid purification by Walls, Dermot, Loughran, Sinéad T

“…Protein fusion technology has enormously facilitated the efficient production and purification of individual recombinant proteins. The use of genetically…”
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Protein Quantitation and Analysis of Purity by Campion, Eva M, Loughran, Sinéad T, Walls, Dermot

“…The accurate quantitation of proteins and an analysis of their purity are essential in numerous areas of scientific research, and is a critical factor in many…”
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A Synopsis of Proteins and Their Purification by Walls, Dermot, Cooney, Gary, Loughran, Sinéad T

“…The isolation of a given protein, free of all other biomolecules, is the primary objective of any protein purification scheme. Classical chromatographic…”
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Influenza infection directly alters innate IL-23 and IL-12p70 and subsequent IL-17A and IFN-γ responses to pneumococcus in vitro in human monocytes by Loughran, Sinead T, Power, Patrick A, Maguire, Paula T, McQuaid, Samantha L, Buchanan, Paul J, Jonsdottir, Ingileif, Newman, Robert W, Harvey, Ruth, Johnson, Patricia A

“…Influenza virus is highly contagious and poses substantial public health problems due to its strong association with morbidity and mortality. Approximately…”
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Syntaxin-4 is essential for IgE secretion by plasma cells by Rahman, Arman, DeCourcey, Joseph, Larbi, Nadia Ben, Loughran, Sinéad T., Walls, Dermot, Loscher, Christine E.

“…•Knock-down of syntaxin-4 in U266 plasma cells resulted in reduction of IgE secretion.•Knock-down of syntaxin-4 also leads to the accumulation of IgE in the…”
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A feasibility study of an exercise intervention to educate and promote health and well-being among medical students: the 'MED-WELL' programme by Worobetz, Aubree, Retief, Petrus J, Loughran, Sinead, Walsh, Jane, Casey, Monica, Hayes, Peter, Bengoechea, Enrique García, O'Regan, Andrew, Woods, Catherine, Kelly, Dervla, Connor, Raymond O, Grath, Deirdre Mc, Glynn, Liam G

“…Medical School programme workloads challenge the physical and mental health of students particularly in compressed graduate entry programmes. There is evidence…”
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Evaluation of a coaching workshop for the management of veterinary nursing students' OSCE-associated test anxiety by Dunne, Karen, Moffett, Jenny, Loughran, Sinead T, Duggan, Vivienne, Campion, Deirdre P

“…High stress levels amongst undergraduates (particularly in relation to assessment) and efforts to improve mental wellbeing have been increasingly reported in…”
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Repression of the Proapoptotic Cellular BIK/NBK Gene by Epstein-Barr Virus Antagonizes Transforming Growth Factor beta 1-Induced B-Cell Apoptosis by Campion, Eva M, Hakimjavadi, Roya, Loughran, Sinead T, Phelan, Susan, Smith, Sinead M, D'Souza, Brendan N, Tierney, Rosemary J, Bell, Andrew I, Cahill, Paul A, Walls, Dermot

“…The Epstein-Barr virus (EBV) establishes a lifelong latent infection in humans. EBV infection of primary B cells causes cell activation and proliferation, a…”
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Purification of poly-histidine-tagged proteins by Loughran, Sinéad T, Walls, Dermot

“…His-tagging is the most widespread and versatile strategy used to purify recombinant proteins for biochemical and structural studies. Recombinant DNA methods…”
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New approaches in the detection of calcium-containing microcrystals in synovial fluid by Hernandez-Santana, Aaron, Yavorskyy, Alexander, Loughran, Sinéad T, McCarthy, Geraldine M, McMahon, Gillian P

“…The presence of calcium phosphate crystals such as basic calcium phosphate and calcium pyrophosphate dihydrate in intra-articular fluid is linked to a number…”
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A TLR5 mono-agonist restores inhibited immune responses to Streptococcus pneumoniae during influenza virus infection in human monocytes by Maguire, Paula T, Loughran, Sinéad T, Harvey, Ruth, Johnson, Patricia A

“…Influenza A virus (IAV) predisposes individuals to often more severe secondary bacterial infections with Streptococcus pneumonia (S. pneumoniae). The outcomes…”
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Modified His-tag fusion vector for enhanced protein purification by immobilized metal affinity chromatography by Loughran, Sinéad T., Loughran, Noeleen B., Ryan, Barry J., D’Souza, Brendan N., Walls, Dermot

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Protein quantitation and analysis of purity by Campion, Eva M, Loughran, Sinéad T, Walls, Dermot

“…The accurate quantitation of proteins and an analysis of their purity are essential in numerous areas of scientific research, and are a critical factor in many…”
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A digest of protein purification by Walls, Dermot, McGrath, Robert, Loughran, Sinéad T

“…The isolation of a given protein, free of all other biomolecules, is the primary objective of any protein purification scheme. Classical chromatographic…”
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In-vitro characterisation of a novel celecoxib microbead formulation for the treatment and prevention of colorectal cancer by McDonald, Bernard F., Quinn, Alison M., Devers, Tomas, Cullen, Alan, Coulter, Ivan S., Marison, Ian W., Loughran, Sinéad T.

“…Objectives Colorectal cancer (CRC) is a life‐threatening disease that can develop as a consequence of a sustained chronic inflammatory pathology of the colon…”
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Functional consequence of positive selection revealed through rational mutagenesis of human myeloperoxidase by Loughran, Noeleen B, Hinde, Sara, McCormick-Hill, Sally, Leidal, Kevin G, Bloomberg, Sarah, Loughran, Sinéad T, O'Connor, Brendan, O'Fágáin, Ciarán, Nauseef, William M, O'Connell, Mary J

“…Myeloperoxidase (MPO) is a member of the mammalian heme peroxidase (MHP) multigene family. Whereas all MHPs oxidize specific halides to generate the…”
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Bfl‐1 is a crucial pro‐survival nuclear factor‐κB target gene in Hodgkin/Reed‐Sternberg cells by Loughran, Sinéad T., Campion, Eva M., D'Souza, Brendan N., Smith, Sinéad M., Vrzalikova, Katerina, Wen, Kaisheng, Murray, Paul G., Walls, Dermot

“…Hodgkin/Reed‐Sternberg (H/RS) cells are believed to represent clonal progeny of Germinal Centre B cells that have escaped negative selection by evading…”
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Haemagglutinin-neuraminidase from HPIV3 mediates human NK regulation of T cell proliferation via NKp44 and NKp46 by McQuaid, Samantha, Loughran, Sinead, Power, Patrick, Maguire, Paula, Walls, Dermot, Cusi, Maria Grazia, Orvell, Claes, Johnson, Patricia

“…HPIV3 is a respiratory virus causing airway diseases, including pneumonia, croup, and bronchiolitis, during infancy and childhood. Currently there is no…”
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