Search Results - "Los, Georgyi V"

Match criteria for human cell line authentication: Where do we draw the line? by Capes‐Davis, Amanda, Reid, Yvonne A., Kline, Margaret C., Storts, Douglas R., Strauss, Ethan, Dirks, Wilhelm G., Drexler, Hans G., MacLeod, Roderick A.F., Sykes, Gregory, Kohara, Arihiro, Nakamura, Yukio, Elmore, Eugene, Nims, Raymond W., Alston‐Roberts, Christine, Barallon, Rita, Los, Georgyi V., Nardone, Roland M., Price, Paul J., Steuer, Anton, Thomson, Jim, Masters, John R.W., Kerrigan, Liz

“…Continuous human cell lines have been used extensively as models for biomedical research. In working with these cell lines, researchers are often unaware of…”
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Peroxisome Dynamics in Cultured Mammalian Cells by Huybrechts, Sofie J, Van Veldhoven, Paul P, Brees, Chantal, Mannaerts, Guy P, Los, Georgyi V, Fransen, Marc

“…Despite the identification and characterization of various proteins that are essential for peroxisome biogenesis, the origin and the turnover of peroxisomes…”
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Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues by Barallon, Rita, Bauer, Steven R, Butler, John, Capes-Davis, Amanda, Dirks, Wilhelm G, Elmore, Eugene, Furtado, Manohar, Kline, Margaret C, Kohara, Arihiro, Los, Georgyi V, MacLeod, Roderick A. F, Masters, John R. W, Nardone, Mark, Nardone, Roland M, Nims, Raymond W, Price, Paul J, Reid, Yvonne A, Shewale, Jaiprakash, Sykes, Gregory, Steuer, Anton F, Storts, Douglas R, Thomson, Jim, Taraporewala, Zenobia, Alston-Roberts, Christine, Kerrigan, Liz

“…Cell misidentification and cross-contamination have plagued biomedical research for as long as cells have been employed as research tools. Examples of…”
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In Vivo Stable Tumor-Specific Painting in Various Colors Using Dehalogenase-Based Protein-Tag Fluorescent Ligands by Kosaka, Nobuyuki, Ogawa, Mikako, Choyke, Peter L, Karassina, Natasha, Corona, Cesear, McDougall, Mark, Lynch, David T, Hoyt, Clifford C, Levenson, Richard M, Los, Georgyi V, Kobayashi, Hisataka

“…In vivo fluorescence cancer imaging is an important tool in understanding tumor growth and therapeutic monitoring and can be performed either with endogenously…”
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The HaloTag: a novel technology for cell imaging and protein analysis by Los, Georgyi V, Wood, Keith

“…The ability to specifically label proteins with a wide range of optical properties and functionalities can help reveal information about protein functions and…”
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Direct pH measurements by using subcellular targeting of 5(and 6-) carboxyseminaphthorhodafluor in mammalian cells by Benink, Hélène, McDougall, Mark, Klaubert, Dieter, Los, Georgyi

“…As a means of reliably measuring intracellular pH, we have precisely targeted 5(and 6-) carboxyseminaphthorhodafluor to cellular subcompartments. This was…”
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Fluorogenic affinity label for the facile, rapid imaging of proteins in live cells by Watkins, Rex W, Lavis, Luke D, Kung, Vanessa M, Los, Georgyi V, Raines, Ronald T

“…Haloalkane dehalogenase (HD) catalyzes the hydrolysis of haloalkanes via a covalent enzyme-substrate intermediate. Fusing a target protein to an HD variant…”
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HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis by Los, Georgyi V, Encell, Lance P, McDougall, Mark G, Hartzell, Danette D, Karassina, Natasha, Zimprich, Chad, Wood, Monika G, Learish, Randy, Ohana, Rachel Friedman, Urh, Marjeta, Simpson, Dan, Mendez, Jacqui, Zimmerman, Kris, Otto, Paul, Vidugiris, Gediminas, Zhu, Ji, Darzins, Aldis, Klaubert, Dieter H, Bulleit, Robert F, Wood, Keith V

“…We have designed a modular protein tagging system that allows different functionalities to be linked onto a single genetic fusion, either in solution, in…”
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In Vivo Labeling Method Using a Genetic Construct for Nanoscale Resolution Microscopy by Schröder, Jan, Benink, Hélène, Dyba, Marcus, Los, Georgyi V.

“…We demonstrate beam scanning-stimulated emission depletion microscopy with in vivo labeled cells. A red emitting fluorescent dye is introduced into membrane…”
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Chromophore-Assisted Light Inactivation of HaloTag Fusion Proteins Labeled with Eosin in Living Cells by Takemoto, Kiwamu, Matsuda, Tomoki, McDougall, Mark, Klaubert, Dieter H, Hasegawa, Akira, Los, Georgyi V, Wood, Keith V, Miyawaki, Atsushi, Nagai, Takeharu

“…Chromophore-assisted light inactivation (CALI) is a potentially powerful tool for the acute disruption of a target protein inside living cells with high…”
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Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter by Svendsen, Soshana, Zimprich, Chad, McDougall, Mark G, Klaubert, Dieter H, Los, Georgyi V

“…The ability to specifically label proteins within living cells can provide information about their dynamics and function. To study a membrane protein, we fused…”
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Cell line misidentification: the beginning of the end by Masters, John R W

“…That a substantial proportion of cell lines is mislabelled or replaced by cells derived from a different individual, tissue or species has been a long known,…”
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Development of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands by Encell, Lance P, Friedman Ohana, Rachel, Zimmerman, Kris, Otto, Paul, Vidugiris, Gediminas, Wood, Monika G, Los, Georgyi V, McDougall, Mark G, Zimprich, Chad, Karassina, Natasha, Learish, Randall D, Hurst, Robin, Hartnett, James, Wheeler, Sarah, Stecha, Pete, English, Jami, Zhao, Kate, Mendez, Jacqui, Benink, Hélène A, Murphy, Nancy, Daniels, Danette L, Slater, Michael R, Urh, Marjeta, Darzins, Aldis, Klaubert, Dieter H, Bulleit, Robert F, Wood, Keith V

“…Our fundamental understanding of proteins and their biological significance has been enhanced by genetic fusion tags, as they provide a convenient method for…”
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Abstract 4305: Biodistribution and clearance of non-targeted DyLight dyes in tumor-free nude mice for in vivo imaging by NLEND, Marie C., Desai, Surbhi, Hong, Suk J., Anderson, Mary Beth, Los, Georgyi V., Hermanson, Greg T., Diener, Justin M., Leevy, Warren M., Bell, Peter A.

“…Abstract Far red to near infra red (NIR) fluorophores are commonly used in cell-based assays and for deep tissue in vivo imaging. The light emitting and…”
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A novel action of lithium: Stimulation of glutamate release and inositol 1,4,5 trisphosphate accumulation via activation of the N-methyl D-aspartate receptor in monkey and mouse cerebral cortex slices by Hokin, Lowell E., Dixon, John F., Los, Georgyi V.

“…Beginning at therapeutic concentrations (1–1.5mM), the anti-manicdepressive drug, lithium, stimulated the release of the major excitatory central…”
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Lithium Stimulates Glutamate "Release" and Inositol 1,4,5-Trisphosphate Accumulation via Activation of the N-Methyl-D-Aspartate Receptor in Monkey and Mouse Cerebral Cortex Slices by Dixon, John F., Los, Georgyi V., Hokin, Lowell E.

“…Beginning at therapeutic concentrations (1-1.5 mM), the anti-manic-depressive drug lithium stimulated the release of glutamate, a major excitatory…”
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Lithium stimulates glutamate "release" and inositol 1,4,5-triphosphate accumulation via activation of the N-methyl-D-aspartate receptor in monkey and mouse cerebral cortex slices by Dixon, John F, Los, Georgyi V, Hokin, Lowell E

“…A study found that the anti-manic-depressive drug lithium stimulated the release of glutamate in monkey cerebral cortex slices with an associated increase in…”
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Lithium enhances accumulation of [3H]inositol radioactivity and mass of second messenger inositol 1,4,5-trisphosphate in monkey cerebral cortex slices by Dixon, J F, Lee, C H, Los, G V, Hokin, L E

“…We previously reported that lithium, in the presence of acetylcholine, increased accumulations of inositol 1,4,5-trisphosphate and inositol…”
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Phosphoinositide signalling in human neuroblastoma cells: Biphasic effect of Li + on the level of the inositolphosphate second messengers by Los, Georgyi V., Artemenko, Irina P., Hokin, Lowell E.

“…Lithium has a biphasic effect on the agonist-dependent accumulation of Ins(1,4,5)P 3 in human neuroblastoma SH-SY5Y cells. These effects consist of a transient…”
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