Search Results - "Lorsch, Jon R."

A mechanistic overview of translation initiation in eukaryotes by Aitken, Colin Echeverría, Lorsch, Jon R

“…Translation initiation requires the formation of a pre-initiation complex that recruits the 5′ end of the mRNA and scans along it to locate the start codon…”
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Conformational Differences between Open and Closed States of the Eukaryotic Translation Initiation Complex by Llácer, Jose L., Hussain, Tanweer, Marler, Laura, Aitken, Colin Echeverría, Thakur, Anil, Lorsch, Jon R., Hinnebusch, Alan G., Ramakrishnan, V.

“…Translation initiation in eukaryotes begins with the formation of a pre-initiation complex (PIC) containing the 40S ribosomal subunit, eIF1, eIF1A, eIF3,…”
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Perspective: Sustaining the big-data ecosystem by Bourne, Philip E., Lorsch, Jon R., Green, Eric D.

“…Organizing and accessing biomedical big data will require quite different business models, say Philip E. Bourne, Jon R. Lorsch and Eric D. Green…”
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Structural Changes Enable Start Codon Recognition by the Eukaryotic Translation Initiation Complex by Hussain, Tanweer, Llácer, Jose L., Fernández, Israel S., Munoz, Antonio, Martin-Marcos, Pilar, Savva, Christos G., Lorsch, Jon R., Hinnebusch, Alan G., Ramakrishnan, V.

“…During eukaryotic translation initiation, initiator tRNA does not insert fully into the P decoding site on the 40S ribosomal subunit. This conformation (POUT)…”
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Applied research won't flourish without basic science by Lorsch, Jon R, Tabak, Lawrence A, Bertagnolli, Monica M

“…Three senior figures at the US National Institutes of Health explain why the agency remains committed to supporting basic science and research…”
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Yeast Ded1 promotes 48S translation pre-initiation complex assembly in an mRNA-specific and eIF4F-dependent manner by Gupta, Neha, Lorsch, Jon R, Hinnebusch, Alan G

“…DEAD-box RNA helicase Ded1 is thought to resolve secondary structures in mRNA 5'-untranslated regions (5'-UTRs) that impede 48S preinitiation complex (PIC)…”
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Distinct interactions of eIF4A and eIF4E with RNA helicase Ded1 stimulate translation in vivo by Gulay, Suna, Gupta, Neha, Lorsch, Jon R, Hinnebusch, Alan G

“…Yeast DEAD-box helicase Ded1 stimulates translation initiation, particularly of mRNAs with structured 5'UTRs. Interactions of the Ded1 N-terminal domain (NTD)…”
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eIF1 discriminates against suboptimal initiation sites to prevent excessive uORF translation genome-wide by Zhou, Fujun, Zhang, Hongen, Kulkarni, Shardul D, Lorsch, Jon R, Hinnebusch, Alan G

“…The translation preinitiation complex (PIC) scans the mRNA for an AUG codon in a favorable context. Previous findings suggest that the factor eIF1…”
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Yeast eIF4A enhances recruitment of mRNAs regardless of their structural complexity by Yourik, Paul, Aitken, Colin Echeverría, Zhou, Fujun, Gupta, Neha, Hinnebusch, Alan G, Lorsch, Jon R

“…eIF4A is a DEAD-box RNA-dependent ATPase thought to unwind RNA secondary structure in the 5'-untranslated regions (UTRs) of mRNAs to promote their recruitment…”
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Translational initiation factor eIF5 replaces eIF1 on the 40S ribosomal subunit to promote start-codon recognition by Llácer, Jose Luis, Hussain, Tanweer, Saini, Adesh K, Nanda, Jagpreet Singh, Kaur, Sukhvir, Gordiyenko, Yuliya, Kumar, Rakesh, Hinnebusch, Alan G, Lorsch, Jon R, Ramakrishnan, V

“…In eukaryotic translation initiation, AUG recognition of the mRNA requires accommodation of Met-tRNA in a 'P ' state, which is antagonized by the factor eIF1…”
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Molecular Architecture of a Eukaryotic Translational Initiation Complex by Fernández, Israel S., Bai, Xiao-Chen, Hussain, Tanweer, Kelley, Ann C., Lorsch, Jon R., Ramakrishnan, V., Scheres, Sjors H. W.

“…The last step in eukaryotic translational initiation involves the joining of the large and small subunits of the ribosome, with initiator transfer RNA…”
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THE MOLECULAR MECHANICS OF EUKARYOTIC TRANSLATION by Kapp, Lee D, Lorsch, Jon R

“…Great advances have been made in the past three decades in understanding the molecular mechanics underlying protein synthesis in bacteria, but our…”
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Transcriptome-wide analysis of the function of Ded1 in translation preinitiation complex assembly in a reconstituted in vitro system by Zhou, Fujun, Bocetti, Julie M, Hou, Meizhen, Qin, Daoming, Hinnebusch, Alan G, Lorsch, Jon R

“…We have developed a deep sequencing-based approach, Rec-Seq, that allows simultaneous monitoring of ribosomal 48S preinitiation complex (PIC) formation on…”
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Temperature-dependent regulation of upstream open reading frame translation in S. cerevisiae by Kulkarni, Shardul D, Zhou, Fujun, Sen, Neelam Dabas, Zhang, Hongen, Hinnebusch, Alan G, Lorsch, Jon R

“…Translation of an mRNA in eukaryotes starts at an AUG codon in most cases, but near-cognate codons (NCCs) such as UUG, ACG, and AUU can also be used as start…”
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Coordinated Movements of Eukaryotic Translation Initiation Factors eIF1, eIF1A, and eIF5 Trigger Phosphate Release from eIF2 in Response to Start Codon Recognition by the Ribosomal Preinitiation Complex by Nanda, Jagpreet S., Saini, Adesh K., Muñoz, Antonio M., Hinnebusch, Alan G., Lorsch, Jon R.

“…Accurate recognition of the start codon in an mRNA by the eukaryotic translation preinitiation complex (PIC) is essential for proper gene expression. The…”
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The Eukaryotic Translation Initiation Factors eIF1 and eIF1A Induce an Open Conformation of the 40S Ribosome by Passmore, Lori A., Schmeing, T. Martin, Maag, David, Applefield, Drew J., Acker, Michael G., Algire, Mikkel A., Lorsch, Jon R., Ramakrishnan, V.

“…Initiation of translation is the process by which initiator tRNA and the start codon of mRNA are positioned in the ribosomal P site. In eukaryotes, one of the…”
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eIF1A residues implicated in cancer stabilize translation preinitiation complexes and favor suboptimal initiation sites in yeast by Martin-Marcos, Pilar, Zhou, Fujun, Karunasiri, Charm, Zhang, Fan, Dong, Jinsheng, Nanda, Jagpreet, Kulkarni, Shardul D, Sen, Neelam Dabas, Tamame, Mercedes, Zeschnigk, Michael, Lorsch, Jon R, Hinnebusch, Alan G

“…The translation pre-initiation complex (PIC) scans the mRNA for an AUG codon in favorable context, and AUG recognition stabilizes a closed PIC conformation…”
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Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex by Aitken, Colin Echeverría, Beznosková, Petra, Vlčkova, Vladislava, Chiu, Wen-Ling, Zhou, Fujun, Valášek, Leoš Shivaya, Hinnebusch, Alan G, Lorsch, Jon R

“…Eukaryotic translation initiation factor 3 (eIF3) is a central player in recruitment of the pre-initiation complex (PIC) to mRNA. We probed the effects on mRNA…”
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Kinetic and thermodynamic analysis of the role of start codon/anticodon base pairing during eukaryotic translation initiation by Kolitz, Sarah E, Takacs, Julie E, Lorsch, Jon R

“…Start codon recognition is a crucial event in the initiation of protein synthesis. To gain insight into the mechanism of start codon recognition in eukaryotes,…”
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eIF1 Controls Multiple Steps in Start Codon Recognition during Eukaryotic Translation Initiation by Nanda, Jagpreet S., Cheung, Yuen-Nei, Takacs, Julie E., Martin-Marcos, Pilar, Saini, Adesh K., Hinnebusch, Alan G., Lorsch, Jon R.

“…Eukaryotic translation initiation factor (eIF) 1 is a central mediator of start codon recognition. Dissociation of eIF1 from the preinitiation complex (PIC)…”
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