Search Results - "Lorsch, Jon"

A mechanistic overview of translation initiation in eukaryotes by Aitken, Colin Echeverría, Lorsch, Jon R

“…Translation initiation requires the formation of a pre-initiation complex that recruits the 5′ end of the mRNA and scans along it to locate the start codon…”
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Fixing problems with cell lines by Lorsch, Jon R, Collins, Francis S, Lippincott-Schwartz, Jennifer

“…Technologies and policies can improve authentication Despite the important role of cell culture in the study of biology and medicine, evidence has accumulated…”
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Conformational Differences between Open and Closed States of the Eukaryotic Translation Initiation Complex by Llácer, Jose L., Hussain, Tanweer, Marler, Laura, Aitken, Colin Echeverría, Thakur, Anil, Lorsch, Jon R., Hinnebusch, Alan G., Ramakrishnan, V.

“…Translation initiation in eukaryotes begins with the formation of a pre-initiation complex (PIC) containing the 40S ribosomal subunit, eIF1, eIF1A, eIF3,…”
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Maximizing the return on taxpayers' investments in fundamental biomedical research by Lorsch, Jon R

“…The National Institute of General Medical Sciences (NIGMS) at the U.S. National Institutes of Health has an annual budget of more than $2.3 billion. The…”
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Perspective: Sustaining the big-data ecosystem by Bourne, Philip E., Lorsch, Jon R., Green, Eric D.

“…Organizing and accessing biomedical big data will require quite different business models, say Philip E. Bourne, Jon R. Lorsch and Eric D. Green…”
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Structural Changes Enable Start Codon Recognition by the Eukaryotic Translation Initiation Complex by Hussain, Tanweer, Llácer, Jose L., Fernández, Israel S., Munoz, Antonio, Martin-Marcos, Pilar, Savva, Christos G., Lorsch, Jon R., Hinnebusch, Alan G., Ramakrishnan, V.

“…During eukaryotic translation initiation, initiator tRNA does not insert fully into the P decoding site on the 40S ribosomal subunit. This conformation (POUT)…”
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The mechanism of eukaryotic translation initiation: new insights and challenges by Hinnebusch, Alan G, Lorsch, Jon R

“…Translation initiation in eukaryotes is a highly regulated and complex stage of gene expression. It requires the action of at least 12 initiation factors, many…”
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Applied research won't flourish without basic science by Lorsch, Jon R, Tabak, Lawrence A, Bertagnolli, Monica M

“…Three senior figures at the US National Institutes of Health explain why the agency remains committed to supporting basic science and research…”
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Functional interplay between DEAD-box RNA helicases Ded1 and Dbp1 in preinitiation complex attachment and scanning on structured mRNAs in vivo by Sen, Neelam Dabas, Gupta, Neha, K Archer, Stuart, Preiss, Thomas, Lorsch, Jon R, Hinnebusch, Alan G

“…RNA structures that impede ribosome binding or subsequent scanning of the 5'-untranslated region (5'-UTR) for the AUG initiation codon reduce translation…”
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Yeast Ded1 promotes 48S translation pre-initiation complex assembly in an mRNA-specific and eIF4F-dependent manner by Gupta, Neha, Lorsch, Jon R, Hinnebusch, Alan G

“…DEAD-box RNA helicase Ded1 is thought to resolve secondary structures in mRNA 5'-untranslated regions (5'-UTRs) that impede 48S preinitiation complex (PIC)…”
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Distinct interactions of eIF4A and eIF4E with RNA helicase Ded1 stimulate translation in vivo by Gulay, Suna, Gupta, Neha, Lorsch, Jon R, Hinnebusch, Alan G

“…Yeast DEAD-box helicase Ded1 stimulates translation initiation, particularly of mRNAs with structured 5'UTRs. Interactions of the Ded1 N-terminal domain (NTD)…”
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eIF1 discriminates against suboptimal initiation sites to prevent excessive uORF translation genome-wide by Zhou, Fujun, Zhang, Hongen, Kulkarni, Shardul D, Lorsch, Jon R, Hinnebusch, Alan G

“…The translation preinitiation complex (PIC) scans the mRNA for an AUG codon in a favorable context. Previous findings suggest that the factor eIF1…”
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Yeast eIF4A enhances recruitment of mRNAs regardless of their structural complexity by Yourik, Paul, Aitken, Colin Echeverría, Zhou, Fujun, Gupta, Neha, Hinnebusch, Alan G, Lorsch, Jon R

“…eIF4A is a DEAD-box RNA-dependent ATPase thought to unwind RNA secondary structure in the 5'-untranslated regions (UTRs) of mRNAs to promote their recruitment…”
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Translational initiation factor eIF5 replaces eIF1 on the 40S ribosomal subunit to promote start-codon recognition by Llácer, Jose Luis, Hussain, Tanweer, Saini, Adesh K, Nanda, Jagpreet Singh, Kaur, Sukhvir, Gordiyenko, Yuliya, Kumar, Rakesh, Hinnebusch, Alan G, Lorsch, Jon R, Ramakrishnan, V

“…In eukaryotic translation initiation, AUG recognition of the mRNA requires accommodation of Met-tRNA in a 'P ' state, which is antagonized by the factor eIF1…”
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Rps3/uS3 promotes mRNA binding at the 40S ribosome entry channel and stabilizes preinitiation complexes at start codons by Dong, Jinsheng, Aitken, Colin Echeverría, Thakur, Anil, Shin, Byung-Sik, Lorsch, Jon R., Hinnebusch, Alan G.

“…The eukaryotic 43S preinitiation complex (PIC) bearing Met-tRNAi Met in a ternary complex (TC) with eukaryotic initiation factor (eIF)2-GTP scans the mRNA…”
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Molecular Architecture of a Eukaryotic Translational Initiation Complex by Fernández, Israel S., Bai, Xiao-Chen, Hussain, Tanweer, Kelley, Ann C., Lorsch, Jon R., Ramakrishnan, V., Scheres, Sjors H. W.

“…The last step in eukaryotic translational initiation involves the joining of the large and small subunits of the ribosome, with initiator transfer RNA…”
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THE MOLECULAR MECHANICS OF EUKARYOTIC TRANSLATION by Kapp, Lee D, Lorsch, Jon R

“…Great advances have been made in the past three decades in understanding the molecular mechanics underlying protein synthesis in bacteria, but our…”
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Developing a culture of safety in biomedical research training by Bond, Michelle R, Gammie, Alison E, Lorsch, Jon R

“…The National Institute of General Medical Sciences (NIGMS) at the U.S. National Institutes of Health (NIH) is committed to supporting the safety of the…”
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Pi release from eIF2, not GTP hydrolysis, is the step controlled by start-site selection during eukaryotic translation initiation by Algire, Mikkel A, Maag, David, Lorsch, Jon R

“…Irreversible GTP hydrolysis by eIF2 is a critical step in translation initiation in eukaryotes because it is thought to commit the translational machinery to…”
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Transcriptome-wide analysis of the function of Ded1 in translation preinitiation complex assembly in a reconstituted in vitro system by Zhou, Fujun, Bocetti, Julie M, Hou, Meizhen, Qin, Daoming, Hinnebusch, Alan G, Lorsch, Jon R

“…We have developed a deep sequencing-based approach, Rec-Seq, that allows simultaneous monitoring of ribosomal 48S preinitiation complex (PIC) formation on…”
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