Complete genome sequence of ‘Mycobacterium neoaurum’ NRRL B-3805, an androstenedione (AD) producer for industrial biotransformation of sterols

Complete genome sequence of ‘Mycobacterium neoaurum’ NRRL B-3805, an androstenedione (AD) producer for industrial biotransformation of sterols

•Genome sequence of the 4-androstene-3,17-dione (AD) producer strain ‘M. neoaurum’ NRRL B-3805 (formerly Mycobacterium sp. NRRL B-3805).•Sequence of B-3805 was nearly identical to that of VKM Ac-1815D, but the detected differences determine its higher AD and lower ADD production.•These genome data p...

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Bibliographic Details
Published in:Journal of biotechnology Vol. 224; pp. 64 - 65
Main Authors: Rodríguez‐García, Antonio, Fernández‐Alegre, Estela, Morales, Alejandro, Sola‐Landa, Alberto, Lorraine, Jess, Macdonald, Sandy, Dovbnya, Dmitry, Smith, Margaret C.M., Donova, Marina, Barreiro, Carlos
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 20-04-2016
Subjects:
4-Androstene-3,17-dione
Actinobacteria
ADD
Androstenedione - metabolism
Austenitic stainless steels
Base Composition
Bioconversion
Degradation
Enzymes
Gene sequencing
Genes
Genome sequence
Genome Size
Genome, Bacterial
Genomes
Mycobacterium
Mycobacterium - genetics
Mycobacterium neoaurum
Plasmids - genetics
Pseudogenes
Sequence Analysis, DNA - methods
Steroids
Sterols
Mycobacterium
ADD
AD
Actinobacteria
Genome sequence
4-Androstene-3,17-dione
Steroids
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Summary:•Genome sequence of the 4-androstene-3,17-dione (AD) producer strain ‘M. neoaurum’ NRRL B-3805 (formerly Mycobacterium sp. NRRL B-3805).•Sequence of B-3805 was nearly identical to that of VKM Ac-1815D, but the detected differences determine its higher AD and lower ADD production.•These genome data permit the species-level identification of the strain using three marker sequences as Mycobacterium neoaurum. Microbial bioconversion of sterols into high value steroid precursors, such as 4-androstene-3,17-dione (AD), is an industrial challenge. Genes and enzymes involved in sterol degradation have been proposed, although the complete pathway is not yet known. The genome sequencing of the AD producer strain ‘Mycobacterium neoaurum’ NRRL B-3805 (formerly Mycobacterium sp. NRRL B-3805) will serve to elucidate the critical steps for industrial processes and will provide the basis for further genetic engineering. The genome comprises a circular chromosome (5 421 338bp), is devoid of plasmids and contains 4844 protein-coding genes.
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ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2016.03.021