Search Results - "Lord, Samuel J."

SuperPlots: Communicating reproducibility and variability in cell biology by Lord, Samuel J, Velle, Katrina B, Mullins, R Dyche, Fritz-Laylin, Lillian K

“…P values and error bars help readers infer whether a reported difference would likely recur, with the sample size n used for statistical tests representing…”
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Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function by Pavani, Sri Rama Prasanna, Thompson, Michael A, Biteen, Julie S, Lord, Samuel J, Liu, Na, Twieg, Robert J, Piestun, Rafael, Moerner, W.E

“…We demonstrate single-molecule fluorescence imaging beyond the optical diffraction limit in 3 dimensions with a wide-field microscope that exhibits a…”
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Adhesion pilus retraction powers twitching motility in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius by Charles-Orszag, Arthur, van Wolferen, Marleen, Lord, Samuel J., Albers, Sonja-Verena, Mullins, R. Dyche

“…Type IV pili are filamentous appendages found in most bacteria and archaea, where they can support functions such as surface adhesion, DNA uptake, aggregation,…”
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High-Temperature Live-Cell Imaging of Cytokinesis, Cell Motility, and Cell-Cell Interactions in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius by Charles-Orszag, Arthur, Lord, Samuel J, Mullins, R Dyche

“…Significant technical challenges have limited the study of extremophile cell biology. Here we describe a system for imaging samples at 75°C using high…”
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Actin-based protrusions of migrating neutrophils are intrinsically lamellar and facilitate direction changes by Fritz-Laylin, Lillian K, Riel-Mehan, Megan, Chen, Bi-Chang, Lord, Samuel J, Goddard, Thomas D, Ferrin, Thomas E, Nicholson-Dykstra, Susan M, Higgs, Henry, Johnson, Graham T, Betzig, Eric, Mullins, R Dyche

“…Leukocytes and other amoeboid cells change shape as they move, forming highly dynamic, actin-filled pseudopods. Although we understand much about the…”
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A Photoactivatable Push−Pull Fluorophore for Single-Molecule Imaging in Live Cells by Lord, Samuel J, Conley, Nicholas R, Lee, Hsiao-lu D, Samuel, Reichel, Liu, Na, Twieg, Robert J, Moerner, W. E

“…We have reengineered a red-emitting dicyanomethylenedihydrofuran push−pull fluorophore so that it is dark until photoactivated with a short burst of…”
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Spatial Organization of EphA2 at the Cell-Cell Interface Modulates Trans-Endocytosis of EphrinA1 by Greene, Adrienne C., Lord, Samuel J., Tian, Aiwei, Rhodes, Christopher, Kai, Hiroyuki, Groves, Jay T.

“…EphA2 is a receptor tyrosine kinase (RTK) that is sensitive to spatial and mechanical aspects of the cell’s microenvironment. Misregulation of EphA2 occurs in…”
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Tadpole Conformation of Gradient Polymer Brushes by Lord, Samuel J, Sheiko, Sergei S, LaRue, Isaac, Lee, Hyung-Il, Matyjaszewski, Krzysztof

“…Cylindrical molecular brushes, each with a gradient of grafting density along the backbone, demonstrated a transition from rodlike to tadpole conformations…”
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WASP and SCAR are evolutionarily conserved in actin-filled pseudopod-based motility by Fritz-Laylin, Lillian K, Lord, Samuel J, Mullins, R Dyche

“…Diverse eukaryotic cells crawl through complex environments using distinct modes of migration. To understand the underlying mechanisms and their evolutionary…”
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Photophysical Properties of Acene DCDHF Fluorophores:  Long-Wavelength Single-Molecule Emitters Designed for Cellular Imaging by Lord, Samuel J., Lu, Zhikuan, Wang, Hui, Willets, Katherine A., Schuck, P. James, Lee, Hsiao-lu D., Nishimura, Stefanie Y., Twieg, Robert J., Moerner, W. E.

“…We report the solvatochromic, viscosity-sensitive, and single-molecule photophysics of the fluorophores DCDHF-N-6 and DCDHF-A-6. These molecules are members of…”
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Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution by Yang, Bin, Chen, Xingye, Wang, Yina, Feng, Siyu, Pessino, Veronica, Stuurman, Nico, Cho, Nathan H., Cheng, Karen W., Lord, Samuel J., Xu, Linfeng, Xie, Dan, Mullins, R. Dyche, Leonetti, Manuel D., Huang, Bo

“…We designed an epi-illumination SPIM system that uses a single objective and has a sample interface identical to that of an inverted fluorescence microscope…”
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Sulfolobus acidocaldarius adhesion pili power twitching motility in the absence of a dedicated retraction ATPase by Charles-Orszag, Arthur, van Wolferen, Marleen, Lord, Samuel J., Albers, Sonja-Verena, Mullins, Dyche

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Polymer dynamics of Alp7A reveals how two critical concentrations govern assembly of dynamically unstable actin-like proteins by Petek-Seoane, Natalie A., Rodriguez, Johnny, Derman, Alan I., Royal, Siobhan G., Lord, Samuel J., Lawrence, Rosalie, Pogliano, Joe, Mullins, R. Dyche

“…Dynamically unstable polymers capture and move cellular cargos in bacteria and eukaryotes, but regulation of their assembly remains poorly understood. Here we…”
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Diffusion of Lipid-like Single-Molecule Fluorophores in the Cell Membrane by Nishimura, Stefanie Y, Lord, Samuel J, Klein, Lawrence O, Willets, Katherine A, He, Meng, Lu, Zhikuan, Twieg, Robert J, Moerner, W. E

“…The dicyanomethylenedihydrofuran (DCDHF) class of single-molecule fluorophores contains an amine donor and a dicyanomethylenedihydrofuran acceptor linked by a…”
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Single-Molecule Spectroscopy and Imaging of Biomolecules in Living Cells by Lord, Samuel J, Lee, Hsiao-lu D, Moerner, W. E

“…The number of reports per year on single-molecule imaging experiments has grown roughly exponentially since the first successful efforts to optically detect a…”
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Superresolution Imaging of Targeted Proteins in Fixed and Living Cells Using Photoactivatable Organic Fluorophores by Lee, Hsiao-lu D, Lord, Samuel J, Iwanaga, Shigeki, Zhan, Ke, Xie, Hexin, Williams, Jarrod C, Wang, Hui, Bowman, Grant R, Goley, Erin D, Shapiro, Lucy, Twieg, Robert J, Rao, Jianghong, Moerner, W. E

“…Superresolution imaging techniques based on sequential imaging of sparse subsets of single molecules require fluorophores whose emission can be photoactivated…”
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Quantitative Proteomic Analysis Reveals apoE4-Dependent Phosphorylation of the Actin-Regulating Protein VASP by Cakir, Zeynep, Lord, Samuel J., Zhou, Yuan, Jang, Gwendolyn M., Polacco, Benjamin J., Eckhardt, Manon, Jimenez-Morales, David, Newton, Billy W., Orr, Adam L., Johnson, Jeffrey R., da Cruz, Alexandre, Mullins, R. Dyche, Krogan, Nevan J., Mahley, Robert W., Swaney, Danielle L.

“…Apolipoprotein (apo) E4 is the major genetic risk factor for Alzheimer’s disease. While neurons generally produce a minority of the apoE in the central nervous…”
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Our evolving view of cell motility by Fritz-Laylin, Lillian K., Lord, Samuel J., Mullins, R. Dyche

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Concise Language Promotes Clear Thinking about Cell Shape and Locomotion by Fritz‐Laylin, Lillian K., Lord, Samuel J., Kakley, Mallory, Mullins, R. Dyche

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Azido Push−Pull Fluorogens Photoactivate to Produce Bright Fluorescent Labels by Lord, Samuel J, Lee, Hsiao-lu D, Samuel, Reichel, Weber, Ryan, Liu, Na, Conley, Nicholas R, Thompson, Michael A, Twieg, Robert J, Moerner, W. E

“…Dark azido push−pull chromophores have the ability to be photoactivated to produce bright fluorescent labels suitable for single-molecule imaging. Upon…”
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