Effect of process parameters on production of a biopolymer by Rhizobium sp

Effect of process parameters on production of a biopolymer by Rhizobium sp

The production of biopolymers by a Rhizobium strain was studied under batch and bioreactor conditions. The best viscosity levels were obtained under low mannitol concentrations as well as low agitation and aeration conditions. Infrared spectra indicated the presence of chemical groups characteristic...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology Vol. 113; no. 1-3; pp. 639 - 652
Main Authors: Duta, F.P, Franca, F.P. de, Servulo, E.F.C, Lopes, L.M. de A, Costa, A.C.A. da, Barros, A
Format: Journal Article
Language:English
Published: United States Springer Nature B.V 2004
Subjects:
Aeration
Agar - chemistry
Agitation
Bacteria
biological production
Biomass
Biopolymers
Biopolymers - chemistry
Bioreactors
Biotechnology - methods
conversion factor
Culture Media
Design of experiments
Exopolysaccharides
Experimental design
Fermentation
Hydrogen-Ion Concentration
Infrared spectra
Mannitol
Mannitol - chemistry
Polymers
polysaccharides
Polysaccharides - chemistry
Process parameters
Rhizobium
Rhizobium - metabolism
Spectrophotometry, Infrared
Statistics as Topic
Studies
Substrates
Temperature
Thermogravimetric analysis
Viscosity
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Summary:The production of biopolymers by a Rhizobium strain was studied under batch and bioreactor conditions. The best viscosity levels were obtained under low mannitol concentrations as well as low agitation and aeration conditions. Infrared spectra indicated the presence of chemical groups characteristic of microbially produced biopolymers, including C = O and O-acetyl groups. Thermogravimetric analysis showed the characteristic degradation profiles of the exopolysaccharide produced (T(onset) = 290degreesC). The experimental design showed that a low substrate concentration (10.0 g/L), and low aeration (0.2 vvm) and agitation (200 rpm) levels should be used. The maximum yield of the process was a Yp/s (g/g) of 0.19 +/- 0.1, obtained under optimized conditions.
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ISSN:0273-2289
1559-0291
0273-2289
DOI:10.1385/ABAB:114:1-3:639