Search Results - "Loo, R. R. Ogorzalek"

Amyloid fibrils in FTLD-TDP are composed of TMEM106B and not TDP-43 by Jiang, Yi Xiao, Cao, Qin, Sawaya, Michael R., Abskharon, Romany, Ge, Peng, DeTure, Michael, Dickson, Dennis W., Fu, Janine Y., Ogorzalek Loo, Rachel R., Loo, Joseph A., Eisenberg, David S.

“…Frontotemporal lobar degeneration (FTLD) is the third most common neurodegenerative condition after Alzheimer’s and Parkinson’s diseases 1 . FTLD typically…”
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Added Value of Internal Fragments for Top-Down Mass Spectrometry of Intact Monoclonal Antibodies and Antibody–Drug Conjugates by Wei, Benqian, Lantz, Carter, Liu, Weijing, Viner, Rosa, Ogorzalek Loo, Rachel R., Campuzano, Iain D. G., Loo, Joseph A.

“…Monoclonal antibodies (mAbs) and antibody–drug conjugates (ADCs) are two of the most important therapeutic drug classes that require extensive…”
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What Protein Charging (and Supercharging) Reveal about the Mechanism of Electrospray Ionization by Ogorzalek Loo, Rachel R., Lakshmanan, Rajeswari, Loo, Joseph A.

“…Understanding the charging mechanism of electrospray ionization is central to overcoming shortcomings such as ion suppression or limited dynamic range, and…”
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Salt Bridge Rearrangement (SaBRe) Explains the Dissociation Behavior of Noncovalent Complexes by Loo, Rachel R. Ogorzalek, Loo, Joseph A.

“…Native electrospray ionization-mass spectrometry, with gas-phase activation and solution compositions that partially release subcomplexes, can elucidate…”
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Structure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions by Jiang, Jiansen, Chan, Henry, Cash, Darian D., Miracco, Edward J., Loo, Rachel R. Ogorzalek, Upton, Heather E., Cascio, Duilio, Johnson, Reid O'Brien, Collins, Kathleen, Loo, Joseph A., Zhou, Z. Hong, Feigon, Juli

“…Telomerase helps maintain telomeres by processive synthesis of telomere repeat DNA at their 3'-ends, using an integral telomerase RNA (TER) and telomerase…”
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Elucidating the molecular programming of a nonlinear non-ribosomal peptide synthetase responsible for fungal siderophore biosynthesis by Jenner, Matthew, Hai, Yang, Nguyen, Hong H., Passmore, Munro, Skyrud, Will, Kim, Junyong, Garg, Neil K., Zhang, Wenjun, Ogorzalek Loo, Rachel R., Tang, Yi

“…Siderophores belonging to the ferrichrome family are essential for the viability of fungal species and play a key role for virulence of numerous pathogenic…”
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Structural Characterization of a Thrombin-Aptamer Complex by High Resolution Native Top-Down Mass Spectrometry by Zhang, Jiang, Loo, Rachel R. Ogorzalek, Loo, Joseph A.

“…Native mass spectrometry (MS) with electrospray ionization (ESI) has evolved as an invaluable tool for the characterization of intact native proteins and…”
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Improving Proteome Coverage and Sample Recovery with Enhanced FASP (eFASP) for Quantitative Proteomic Experiments by Erde, Jonathan, Loo, Rachel R Ogorzalek, Loo, Joseph A

“…Enhanced Filter Aided Sample Preparation (eFASP) incorporates plastics passivation and digestion-enhancing surfactants into the traditional FASP workflow to…”
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Structure-based design of nanobodies that inhibit seeding of Alzheimer's patient-extracted tau fibrils by Abskharon, Romany, Pan, Hope, Sawaya, Michael R, Seidler, Paul M, Olivares, Eileen J, Chen, Yu, Murray, Kevin A, Zhang, Jeffrey, Lantz, Carter, Bentzel, Megan, Boyer, David R, Cascio, Duilio, Nguyen, Binh A, Hou, Ke, Cheng, Xinyi, Pardon, Els, Williams, Christopher K, Nana, Alissa L, Vinters, Harry V, Spina, Salvatore, Grinberg, Lea T, Seeley, William W, Steyaert, Jan, Glabe, Charles G, Ogorzalek Loo, Rachel R, Loo, Joseph A, Eisenberg, David S

“…Despite much effort, antibody therapies for Alzheimer's disease (AD) have shown limited efficacy. Challenges to the rational design of effective antibodies…”
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New Reagents for Increasing ESI Multiple Charging of Proteins and Protein Complexes by Lomeli, Shirley H., Peng, Ivory X., Yin, Sheng, Ogorzalek Loo, Rachel R., Loo, Joseph A.

“…The addition of m-nitrobenzyl alcohol ( m-NBA) was shown previously (Lomeli et al., J. Am. Soc. Mass Spectrom. 2009, 20, 593–596) to enhance multiple charging…”
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Increasing Charge While Preserving Noncovalent Protein Complexes for ESI-MS by Lomeli, Shirley H., Yin, Sheng, Ogorzalek Loo, Rachel R., Loo, Joseph A.

“…Increased multiple charging of native proteins and noncovalent protein complexes is observed in electrospray ionization (ESI) mass spectra obtained from…”
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Top-down/Bottom-up Mass Spectrometry Workflow Using Dissolvable Polyacrylamide Gels by Takemori, Nobuaki, Takemori, Ayako, Wongkongkathep, Piriya, Nshanian, Michael, Loo, Rachel R. Ogorzalek, Lermyte, Frederik, Loo, Joseph A

“…Biologists’ preeminent toolbox for separating, analyzing, and visualizing proteins is SDS-PAGE, yet recovering the proteins embedded in these polyacrylamide…”
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Enhancing protein disulfide bond cleavage by UV excitation and electron capture dissociation for top-down mass spectrometry by Wongkongkathep, Piriya, Li, Huilin, Zhang, Xing, Ogorzalek Loo, Rachel R., Julian, Ryan R., Loo, Joseph A.

“…•UV-activation (266 nm) can homolytically cleave a disulfide bond.•UV-activation with ECD allowed the three disulfide bonds of insulin to be cleaved.•For…”
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Increasing fragmentation of disulfide-bonded proteins for top–down mass spectrometry by supercharging by Zhang, Jiang, Loo, Rachel R. Ogorzalek, Loo, Joseph A.

“…[Display omitted] •Supercharging (sulfolane) increases the ESI charging of disulfide-bonded proteins.•ECD/CAD of the higher charge states allows cleavage of…”
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Keystone predation and molecules of keystone significance by Zimmer, Richard K., Ferrier, Graham A., Kim, Steven J., Loo, Rachel R. Ogorzalek, Zimmer, Cheryl Ann, Loo, Joseph A.

“…Keystone species structure ecological communities and are major determinants of biodiversity. A synthesis of research on keystone species is nonetheless…”
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Dynamic acylome reveals metabolite driven modifications in Syntrophomonas wolfei by Fu, Janine Y, Muroski, John M, Arbing, Mark A, Salguero, Jessica A, Wofford, Neil Q, McInerney, Michael J, Gunsalus, Robert P, Loo, Joseph A, Ogorzalek Loo, Rachel R

“…is an anaerobic syntrophic microbe that degrades short-chain fatty acids to acetate, hydrogen, and/or formate. This thermodynamically unfavorable process…”
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Synthesis of Maleimide-End-Functionalized Star Polymers and Multimeric Protein−Polymer Conjugates by Tao, Lei, Kaddis, Catherine S, Loo, Rachel R. Ogorzalek, Grover, Gregory N, Loo, Joseph A, Maynard, Heather D

“…Protein−polymer conjugates exhibit superior properties to unmodified proteins, generating a high demand for these materials in the fields of medicine,…”
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Membrane Complexes of Syntrophomonas wolfei Involved in Syntrophic Butyrate Degradation and Hydrogen Formation by Crable, Bryan R, Sieber, Jessica R, Mao, Xinwei, Alvarez-Cohen, Lisa, Gunsalus, Robert, Ogorzalek Loo, Rachel R, Nguyen, Hong, McInerney, Michael J

“…Syntrophic butyrate metabolism involves the thermodynamically unfavorable production of hydrogen and/or formate from the high potential electron donor,…”
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An integrated native mass spectrometry and top-down proteomics method that connects sequence to structure and function of macromolecular complexes by Li, Huilin, Nguyen, Hong Hanh, Ogorzalek Loo, Rachel R., Campuzano, Iain D. G., Loo, Joseph A.

“…Mass spectrometry (MS) has become a crucial technique for the analysis of protein complexes. Native MS has traditionally examined protein subunit arrangements,…”
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Reactive-Electrospray-Assisted Laser Desorption/Ionization for Characterization of Peptides and Proteins by Peng, Ivory X, Ogorzalek Loo, Rachel R, Shiea, Jentaie, Loo, Joseph A

“…Electrospray-assisted laser desorption/ionization (ELDI) is a soft ionization method for mass spectrometry (MS) and combines features of both electrospray…”
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