Carnosine supplementation and retinal oxidative parameters in a high-calorie diet rat model

Carnosine supplementation and retinal oxidative parameters in a high-calorie diet rat model

To assess oxidative effects induced by a high-calorie diet on the retina of Wistar rats and test the antioxidative effects of carnosine supplementation. Wistar rats were randomly divided into the following groups: standard diet (SD), high-calorie diet (HcD), standard diet + carnosine (SD + Car), and...

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Published in:BMC ophthalmology Vol. 23; no. 1; p. 502
Main Authors: de Almeida Torres, Rogil Jose, Moreto, Fernando, Luchini, Andrea, de Almeida Torres, Rogerio Joao, Longo, Sofia Pimentel, Pinho, Ricardo Aurino, Nagashima, Seigo, de Noronha, Lucia, Ferron, Artur Junio Togneri, de Almeida Silva, Carol Cristina Vagula, Correa, Camila Renata, Aldini, Giancarlo, Ferreira, Ana Lucia Anjos
Format: Journal Article
Language:English
Published: England BioMed Central Ltd 08-12-2023
BioMed Central
BMC
Subjects:
Adipose tissue
Aldehydes
Animal models
Antioxidant
Antioxidants
Body weight
Calories
Carbohydrates
Carbonyl compounds
Carnosine
Cataracts
Creatinine
Dextrose
Diet
Dietary minerals
Food
Glucose
Glutathione
High density lipoprotein
High-calorie diet
Hydrogen peroxide
Hyperglycemia
Laboratory animals
Lipids
Low density lipoprotein
Low density lipoproteins
Macular degeneration
Medical colleges
Medical research
Metabolic syndrome
Obesity
Oxidation
Oxidative stress
Peptides
Retina
Sorghum
Soybeans
Stress analysis
Sucrose
Sulfhydryl groups
Triglycerides
Uric acid
Retina
Carnosine
High-calorie diet
Antioxidant
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Summary:To assess oxidative effects induced by a high-calorie diet on the retina of Wistar rats and test the antioxidative effects of carnosine supplementation. Wistar rats were randomly divided into the following groups: standard diet (SD), high-calorie diet (HcD), standard diet + carnosine (SD + Car), and high-calorie diet + carnosine (HcD + Car). The body weight, adiposity index, plasma glucose, total lipids, high-density lipoprotein (HDL), low-density lipoprotein (LDL), uric acid, creatinine, and triglycerides of the animals were evaluated. The retinas were analyzed for markers of oxidative stress. Hydrogen peroxide production was assessed by 2',7'-dichlorodihydrofluorescein diacetate (DCF) oxidation. The total glutathione (tGSH), total antioxidant capacity (TAC), protein carbonyl, and sulfhydryl groups of the antioxidant system were analyzed. TAC levels increased in the retinas of the SD + Car group compared to the SD group (p < 0.05) and in the HcD + Car group compared to the HcD group (p < 0.05). The levels of GSH and the GSSH:GSSG ratio were increased in the HcD + Car group compared to the SD + Car group (p < 0.05). An increase in the retinal carbonyl content was observed in the HcD group compared to the SD group (p < 0.05) and in the HcD + Car group compared to the SD + Car group (p < 0.05). A high-calorie diet (HcD) was also associated with a decrease in retinal sulfhydryl-type levels compared to the SD group (p < 0.05). The results suggest that feeding a high-calorie diet to rats can promote an increase in carbonyl content and a reduction in sulfhydryl groups in their retinas. The administration of carnosine was not effective in attenuating these oxidative markers. Animal Ethics Committee of Botucatu Medical School - Certificate number 1292/2019.
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content type line 23
ISSN:1471-2415
1471-2415
DOI:10.1186/s12886-023-03255-y