A transcriptomic approach of salinity response in the euryhaline teleost, Dicentrarchus labrax

Euryhaline teleosts possess the capacity to osmoregulate under various environmental conditions (freshwater to hypersaline water). This physiological capacity is generally monitored using enzyme activity assays (Na +/K +-ATPase…), hormones quantification (prolactine, growth hormone) or their mRNAs e...

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Bibliographic Details
Published in:	Gene Vol. 379; no. 1; pp. 40 - 50
Main Authors:	Boutet, I., Long Ky, C.L., Bonhomme, F.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-09-2006 Elsevier
Subjects:	Adaptation, Physiological Amino Acid Sequence Animals Base Sequence Bass - genetics Bass - physiology Biodiversity and Ecology Brackish Carbonic anhydrase Carbonic Anhydrases - genetics Carbonic Anhydrases - metabolism Cell Cycle Dicentrarchus labrax Environmental Sciences Female Fish Proteins - genetics Fish Proteins - metabolism Fresh Water Freshwater Gene Expression Profiling Genetic Markers Gills - cytology Gills - physiology Intestines - cytology Intestines - physiology Male Marine Metalloendopeptidases - genetics Metalloendopeptidases - immunology Metalloendopeptidases - metabolism Molecular Sequence Data mRNA expression Osmoregulation RNA, Messenger - metabolism Sea bass Seawater Sodium Chloride - metabolism Suppression subtractive hybridisation Transcription, Genetic Water-Electrolyte Balance - genetics RT UTR ACE Carbonic anhydrase RAPD Osmoregulation cDNA Suppression subtractive hybridisation Sea bass CA mRNA expression
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Summary:	Euryhaline teleosts possess the capacity to osmoregulate under various environmental conditions (freshwater to hypersaline water). This physiological capacity is generally monitored using enzyme activity assays (Na +/K +-ATPase…), hormones quantification (prolactine, growth hormone) or their mRNAs expression. To date, few studies addressed the genetic correlates of adaptation to varying salinity at a molecular level in such fish. In the sea bass Dicentrarchus labrax, genetic differentiation was observed at specific allozyme loci between lagoon- and open-sea populations. In the present study, we investigated transcriptomic response of D. labrax to salt- and freshwater acclimation in two organs involved in osmoregulation, gill and intestine. By using suppression subtractive hybridisation, we characterised 586 partial cDNA sequences encoding proteins potentially involved in the metabolism of sea bass acclimated to salt- or freshwater under experimental conditions. Using these results, we first characterised complete genomic sequence of a carbonic anhydrase and then analysed mRNA expression of genes potentially involved in osmoregulation mechanisms (Na +/K +-ATPase, carbonic anhydrase, angiotensin-converting enzyme and claudin-3), cell-cycle regulation (secretagogin) and immune system (nephrosin) in gill and intestine of wild fish from open sea and lagoons. Our analyses indicate a strong tissue- and environmental-dependant expression pattern for all the genes studied. A transcriptomic approach such as described in the present paper provides thus a first description of genes involved in metabolic or structural functions important for coping with environmental salinity variations in a euryhaline fish like the common sea bass D. labrax. It should be supplemented by proteomics to check the direct involvement of the gene products at the protein level, and by polymorphism analyses if one is to understand population or individual fluctuations in acclimation to salinity variation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0378-1119 1879-0038
DOI:	10.1016/j.gene.2006.04.011