Chorismate mutase isoenzymes from selected plants and their immunological comparison with the isoenzymes from Sorghum bicolor

The isoenzyme pattern of chorismate mutase (EC 5.4.99.5) was examined by diethylaminoethyl-cellulose chromatography in a wide variety of plants. All plants contained a regulated form of chorismate mutase (CM-1), and most contained an additional, unregulated form (CM-2). The regulatory properties of...

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Bibliographic Details
Published in:	Plant physiology (Bethesda) Vol. 81; no. 3; pp. 717 - 722
Main Authors:	Singh, B.K, Lonergan, S.G, Conn, E.E
Format:	Journal Article
Language:	English
Published:	Rockville, MD American Society of Plant Physiologists 01-07-1986
Subjects:	Allosteric regulation Analytical, structural and metabolic biochemistry ANATOMIA DE LA PLANTA ANATOMIE VEGETALE ANTIGENIC RESPONSE Antiserum BIOCHEMISTRY BIOCHIMIE Biological and medical sciences BIOQUIMICA Biosynthesis Chromatography Cross reaction Cyclic amino acids Enzymes Enzymes and enzyme inhibitors FONCTION PHYSIOLOGIQUE FUNCION FISIOLOGICA Fundamental and applied biological sciences. Psychology HORDEUM VULGARE Immunology ISOENZIMAS ISOENZYME ISOENZYMES Isomerases PHYSIOLOGICAL FUNCTIONS PLANT ANATOMY PLANTAS PLANTE PLANTS RADIOIMMUNOASSAY Sorghum SORGHUM BICOLOR TECHNIQUE RADIO-IMMUNOLOGIQUE TECNICA RADIOINMUNOLOGICA ZEA MAYS Monocotyledones Sorghum bicolor Vegetals Chorismate mutase Cruciferae Enzyme Gramineae Isozyme Dicotyledones Angiospermae Spermatophyta Immunochemistry
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Summary:	The isoenzyme pattern of chorismate mutase (EC 5.4.99.5) was examined by diethylaminoethyl-cellulose chromatography in a wide variety of plants. All plants contained a regulated form of chorismate mutase (CM-1), and most contained an additional, unregulated form (CM-2). The regulatory properties of CM-1 differed significantly between plants. Antisera prepared against CM-1 and CM-2 from Sorghum bicolor were used to test immunological cross reaction of chorismate mutases from other plants. There was a high degree of similarity between chorismate mutase isoenzymes from Sorghum bicolor and Zea mays and some with Hordeum vulgare, but all other species studied were antigenically distinct from sorghum. No homology between the structure of CM-1 and CM-2 was detected within any species.
Bibliography:	F50 F60 8703894 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0032-0889 1532-2548
DOI:	10.1104/pp.81.3.717