Tn5041: a chimeric mercury resistance transposon closely related to the toluene degradative transposon Tn4651

Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia 1 Author for correspondence: V. G. Nikiforov. Tel: + 7 095 196 0015. Fax: + 7 095 196 0221. e-mail: uimg@img.rc.ac.ru ABSTRACT Summary: This paper reports the discovery and characterization of Tn 5041 , a novel-type...

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Published in:	Microbiology (Society for General Microbiology) Vol. 143; no. 8; pp. 2549 - 2556
Main Authors:	Kholodii, G. Ya, Yurieva, O. V, Gorlenko, Zh. M, Mindlin, S. Z, Bass, I. A, Lomovskay, O. L, Kopteva, A. V, Nikiforov, V. G
Format:	Journal Article
Language:	English
Published:	England Soc General Microbiol 01-08-1997
Subjects:	Amino Acid Sequence Bacterial Proteins - genetics Base Sequence Biodegradation, Environmental Chimera Cloning, Molecular DNA Nucleotidyltransferases - genetics DNA Transposable Elements - genetics Drug Resistance, Microbial - genetics Escherichia coli - genetics Evolution, Molecular Genes, Bacterial Integrases Mercury - pharmacology Molecular Sequence Data Operon Pseudomonas Pseudomonas - drug effects Pseudomonas - genetics Recombinases Sequence Analysis, DNA Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Toluene - metabolism Transposon Resolvases
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Summary:	Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia 1 Author for correspondence: V. G. Nikiforov. Tel: + 7 095 196 0015. Fax: + 7 095 196 0221. e-mail: uimg@img.rc.ac.ru ABSTRACT Summary: This paper reports the discovery and characterization of Tn 5041 , a novel-type transposon vehicle for dissemination of mercury resistance in natural bacterial populations. Tn 5041 (14876 bp), identified in a Pseudomonas strain from a mercury mine, is a Tn3 family mercury resistance transposon far outside the Tn 21 subgroup. As in other Tn3 family transposons, Tn 5041 duplicates 5 bp of the target sequence following insertion. Tn 5041 apparently acquired its mer operon as a single-ended relic of a transposon belonging to the classical mercury resistance transposons of the Tn 21 subgroup. The putative transposase and the 47 bp terminal inverted repeats of Tn 5041 are closely related to those of the toluene degradative transposon Tn 4651 and fall into a distinct subgroup on the fringe of the Tn 3 family. The amino acid sequence of the putative resolvase of Tn 5041 resembles site-specific recombinases of the integrase family. Besides the mer operon and putative transposition genes, Tn 5041 contains a 4 kb region that accommodates a number of apparently defective genes and mobile elements. Keywords: Tn5041, mer operon, Tn3 family, integrase, transposase Present address: Microcide Pharmaceuticals, 850 Maude Ave, Mountain View, CA 94043, USA. Present address: The Rockefeller University, New York, NY 10021, USA.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1350-0872 1465-2080
DOI:	10.1099/00221287-143-8-2549