Immunopotentiating of mucosal and systemic antibody responses in mice by intranasal immunization with HLT-combined influenza virosomal vaccine

The mucosal vaccination strategy against influenza has been investigated by using influenza virosomal vaccine (IRIV) combined with two different adjuvants: the procholeragenoid (PCG) and the Escherichia coli heat labile toxin (HLT). A comparative study has been carried out on mice administered intra...

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Bibliographic Details
Published in:	Vaccine Vol. 18; no. 25; pp. 2838 - 2842
Main Authors:	Cusi, M.G, Lomagistro, M.M, Valassina, M, Valensin, P.E, Glück, R
Format:	Journal Article
Language:	English
Published:	Oxford Elsevier Ltd 15-06-2000 Elsevier
Subjects:	Adjuvants, Immunologic - administration & dosage Administration, Intranasal Animals Antibodies, Bacterial - biosynthesis Antibodies, Viral - biosynthesis Bacterial Toxins - administration & dosage Bacterial Toxins - immunology Biological and medical sciences Cholera Toxin - administration & dosage Cholera Toxin - immunology Enterotoxins - administration & dosage Enterotoxins - immunology Escherichia coli Proteins Female Fundamental and applied biological sciences. Psychology Heat labile toxin Immunity, Mucosal Influenza Vaccines - administration & dosage Influenza Vaccines - adverse effects Influenza Vaccines - immunology Influenza virosome influenza virus Injections, Intramuscular Interferon-gamma - biosynthesis Mice Mice, Inbred BALB C Microbiology Nasal Mucosa - immunology Nasal Mucosa - metabolism Procholeragenoid Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, Combined - administration & dosage Vaccines, Combined - immunology Virology Heat labile toxin Influenza virosome Procholeragenoid Immunostimulation Mucosa Virus like particle Rodentia Orthomyxoviridae Virosome Intranasal administration Polyvalent vaccine Influenzavirus Property formulation relationship Virus Toxin Vertebrata Local immunity Mammalia Immunogenicity Mouse Immunological adjuvant Enterotoxin Cholera toxin Humoral immunity
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Summary:	The mucosal vaccination strategy against influenza has been investigated by using influenza virosomal vaccine (IRIV) combined with two different adjuvants: the procholeragenoid (PCG) and the Escherichia coli heat labile toxin (HLT). A comparative study has been carried out on mice administered intranasally with these different formulations of influenza vaccine. PCG appears less effective than HLT in inducing an IgG response, but both the adjuvants elicit mucosal adjuvant activity inducing s-IgA in the upper respiratory tract. On the contrary, only HLT when administered intranasally to mice with influenza virosomes stimulates the production of s-IgA in the lower respiratory tract thereby providing a better protection against primary infection of the respiratory system. Both HLT and PCG enhance the production of IFN- γ in the respiratory tract, nevertheless HLT appears more efficacious as a mucosal adjuvant.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0264-410X 1873-2518
DOI:	10.1016/S0264-410X(00)00072-4