Characterization of proanthocyanidins from grape seeds

Characterization of proanthocyanidins from grape seeds

Leucoselect™ (grape seed selected proanthocyanidins) was analyzed. HPLC thermospray mass spectrometry (TSP-MS) allowed the detection of monomeric flavan-3-ols and dimeric proanthocyanidins. Fractionation over Sephadex® LH-20 resin and analysis of the isolated fractions by gel permeation chromatograp...

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Bibliographic Details
Published in:Fitoterapia Vol. 71; no. 2; pp. 162 - 175
Main Authors: Gabetta, Bruno, Fuzzati, Nicola, Griffini, Alberto, Lolla, Enrico, Pace, Roberto, Ruffilli, Tiziano, Peterlongo, Federico
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-04-2000
Subjects:
Anthocyanins - chemistry
Chromatography, Gel
Chromatography, High Pressure Liquid
ESI-MS
GPC
Grape seeds
HPLC TSP-MS
Humans
Mass Spectrometry
Proanthocyanidins
Rosales
Seeds
Vitis vinifera
HPLC TSP-MS
ESI-MS
GPC
Grape seeds
Vitis vinifera
Proanthocyanidins
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Summary:Leucoselect™ (grape seed selected proanthocyanidins) was analyzed. HPLC thermospray mass spectrometry (TSP-MS) allowed the detection of monomeric flavan-3-ols and dimeric proanthocyanidins. Fractionation over Sephadex® LH-20 resin and analysis of the isolated fractions by gel permeation chromatography (GPC) and electrospray mass spectrometry (ESI-MS) led to the complete characterization of the proanthocyanidin constituents of Leucoselect™. The analysis revealed the presence of approximately 15% of (+)-catechin (1) and (−)-epicatechin (2), 80% of (−)-epicatechin 3- O-gallate (3), dimers, trimers, tetramers and their gallates and 5% of pentamers, hexamers, heptamers and their gallates.
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ISSN:0367-326X
DOI:10.1016/S0367-326X(99)00161-6