LpxD gene knockout elicits protection to Litopenaeus vannamei, white shrimp, against Vibrio parahaemolyticus infection

LpxD gene knockout elicits protection to Litopenaeus vannamei, white shrimp, against Vibrio parahaemolyticus infection

Vibrio parahaemolyticus is a Gram-negative bacterium causing acute hepatopancreatic necrosis disease (AHPND) in white shrimp, Litopenaeus vannamei . LpxD gene, a principal component of lipid A, which plays a vital role in the biosynthesis of lipopolysaccharides (LPSs) responsible for the pathogenesi...

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Bibliographic Details
Published in:Aquaculture international Vol. 27; no. 5; pp. 1383 - 1393
Main Authors: Tsai, Ching-Yi, Santos, Harvey M., Hu, Shao-Yang, Sang, Cheng Yu, Yanuaria, Ciara Alyssa S., Lola, Ernest Nicolo G., Tayo, Lemmuel L., Nitura, Karmella Marie A., Liu, Chun Hung, Chuang, Kuo Pin
Format: Journal Article
Language:English
Published: Cham Springer International Publishing 01-10-2019
Springer Nature B.V
Subjects:
Bacteria
Biomedical and Life Sciences
Biosynthesis
Decapoda
Disease control
Freshwater & Marine Ecology
Hepatopancreas
Immunogenicity
Life Sciences
Lipids
Litopenaeus vannamei
Necrosis
Pathogens
Protection
Recombination
Seafood
Vaccines
Vibrio parahaemolyticus
Vibriosis
Virulence
Waterborne diseases
Zoology
Acute hepatopancreatic necrosis disease (AHPND)
Oral vaccine
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Summary:Vibrio parahaemolyticus is a Gram-negative bacterium causing acute hepatopancreatic necrosis disease (AHPND) in white shrimp, Litopenaeus vannamei . LpxD gene, a principal component of lipid A, which plays a vital role in the biosynthesis of lipopolysaccharides (LPSs) responsible for the pathogenesis of Gram-negative bacteria However, the function of lpxD in V. parahaemolyticus ’ virulence has not been studied. In this study, lpxD gene knockout and its role in V. parahaemolyticus ’ virulence in white shrimp were determined. The lpxD gene was knocked out by homologous recombination. Virulence and immunogenicity of the lpxD gene was disrupted and the pirA, the major virulence factor of AHPND, and AP1 gene were intact in white shrimp. White shrimp fed with normal diet with VP△ lpxD displayed protection (reduced mortality rate from 66 to 27%) after challenge with V. parahaemolyticus . Our results indicated that knockout of the lpxD gene can cause attenuation of V. parahaemolyticus in white shrimp, and VP△ lpxD could be used as a live attenuated oral vaccine against V. parahaemolyticus in white shrimp.
ISSN:0967-6120
1573-143X
DOI:10.1007/s10499-019-00398-y