Characterization of Insulin-Secreting Porcine Bone Marrow Stromal Cells Ex Vivo and Autologous Cell Therapy In Vivo

Cell therapy could potentially meet the need for pancreas and islet transplantations in diabetes mellitus that far exceeds the number of available donors. Bone marrow stromal cells are widely used in clinical trials mainly for their immunomodulatory effects with a record of safety. However, less foc...

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Bibliographic Details
Published in:	Cell transplantation Vol. 24; no. 7; p. 1205
Main Authors:	Do, Hai Van Thi, Loke, Wan Ting, Kee, Irene, Liang, Vivienne, David, Sebastian J, Gan, Shu Uin, Lee, Sze Sing, Ng, Wai Har, Koong, Heng Nung, Ong, Hock Soo, Lee, Kok Onn, Calne, Roy Y, Kon, Oi Lian
Format:	Journal Article
Language:	English
Published:	United States SAGE Publishing 01-07-2015
Subjects:	Animals Bone Marrow Cells - cytology Bone Marrow Cells - metabolism Cell- and Tissue-Based Therapy Diabetes Mellitus, Experimental Disease Models, Animal Humans Insulin - metabolism Insulin-Secreting Cells - metabolism Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Swine
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Summary:	Cell therapy could potentially meet the need for pancreas and islet transplantations in diabetes mellitus that far exceeds the number of available donors. Bone marrow stromal cells are widely used in clinical trials mainly for their immunomodulatory effects with a record of safety. However, less focus has been paid to developing these cells for insulin secretion by transfection. Although murine models of diabetes have been extensively used in gene and cell therapy research, few studies have shown efficacy in large preclinical animal models. Here we report optimized conditions for ex vivo expansion and characterization of porcine bone marrow stromal cells and their permissive expression of a transfected insulin gene. Our data show that these cells resemble human bone marrow stromal cells in surface antigen expression, are homogeneous, and can be reproducibly isolated from outbred Yorkshire-Landrace pigs. Porcine bone marrow stromal cells were efficiently expanded in vitro to >10(10) cells from 20 ml of bone marrow and remained karyotypically normal during expansion. These cells were electroporated with an insulin expression plasmid vector with high efficiency and viability, and secreted human insulin and C-peptide indicating appropriate processing of proinsulin. We showed that autologous insulin-secreting bone marrow stromal cells implanted and engrafted in the liver of a streptozotocin-diabetic pig that modeled type 1 diabetes resulted in partial, but significant, improvement in hyperglycemia that could not be ascribed to regeneration of endogenous β-cells. Glucose-stimulated insulin secretion in vivo from implanted cells in the treated pig was documented by a rise in serum human C-peptide levels during intravenous glucose tolerance tests. Compared to a sham-treated control pig, this resulted in significantly reduced fasting hyperglycemia, a slower rise in serum fructosamine, and prevented weight loss. Taken together, this study suggests that bone marrow stromal cells merit further development as autologous cell therapy for diabetes.
ISSN:	0963-6897 1555-3892
DOI:	10.3727/096368914X679363