Identification and quantitative confirmation of dinitropyrenes and 3-nitrobenzanthrone as major mutagens in contaminated sediments

Identification and quantitative confirmation of dinitropyrenes and 3-nitrobenzanthrone as major mutagens in contaminated sediments

Polar fractions of a sediment extract of the industrial area of Bitterfeld, Germany, have been subjected for effect-directed identification of mutagens using the Ames fluctuation assay with TA98. Mutagenicity could be well recovered in several secondary and tertiary fractions. Dinitropyrenes and 3-n...

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Bibliographic Details
Published in:Environment international Vol. 44; pp. 31 - 39
Main Authors: Lübcke-von Varel, Urte, Bataineh, Mahmoud, Lohrmann, Stefanie, Löffler, Ivonne, Schulze, Tobias, Flückiger-Isler, Sini, Neca, Jiri, Machala, Miroslav, Brack, Werner
Format: Journal Article
Language:English
Published: Oxford Elsevier Ltd 01-09-2012
Elsevier
Subjects:
Ames fluctuation assay
Aromatic compounds
Benz(a)Anthracenes - analysis
Benz(a)Anthracenes - toxicity
Biological and medical sciences
Chemical mutagenesis
Diagnostic systems
Effect-directed analysis
Environment. Living conditions
Environmental Monitoring - methods
Environmental pollutants toxicology
Geologic Sediments - chemistry
Germany
Humans
Inclusions
LC–(APCI-)MS/MS
Medical sciences
Mutagen identification
Mutagenicity
Mutagenicity Tests
Mutagens
Mutagens - analysis
Mutagens - toxicity
Nitro-PAH
Polycyclic Aromatic Hydrocarbons - analysis
Polycyclic Aromatic Hydrocarbons - toxicity
Public health. Hygiene
Public health. Hygiene-occupational medicine
Risk
Salmonella - drug effects
Sediments
Toxicology
Water Pollutants, Chemical - analysis
Water Pollutants, Chemical - toxicity
Europe
Germany
Germany, Sachsen-Anhalt, Bitterfeld
Mutagen identification
Nitro-PAH
Ames fluctuation assay
LC–(APCI-)MS/MS
Effect-directed analysis
Nitro compound
LC-(APCI-)MS/MS
Toxicity
Genotoxicity
Identification
Mutagen
Soil pollution
Sediments
Mass spectrometry MS/MS
Industrial area
Organic compounds
Quantitative analysis
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Summary:Polar fractions of a sediment extract of the industrial area of Bitterfeld, Germany, have been subjected for effect-directed identification of mutagens using the Ames fluctuation assay with TA98. Mutagenicity could be well recovered in several secondary and tertiary fractions. Dinitropyrenes and 3-nitrobenzanthrone could be confirmed to contribute great shares of the observed mutagenicity. In addition, a multitude of polar polycyclic aromatic compounds has been tentatively identified in mutagenic fractions including nitro-PAHs, azaarenes, ketones, quinones, hydroxy-compounds, lactones and carboxylic acids although their contribution to mutagenicity could not be quantified due to a lack of standards. Diagnostic Salmonella strains YG1024 and YG1041 were applied to confirm the contribution of nitro-aromatic compounds. We suggest the inclusion of dinitropyrenes and 3-nitrobenzanthrone into sediment monitoring in order to minimize the mutagenic risk to aquatic organisms and to human health. ► Effect-directed analysis of polar sediment extract fractions. ► Extensive fractionation. ► Tentative identification of numerous polar polycyclic aromatic compounds. ► Quantitative confirmation of dinitropyrenes and 3-nitrobenzanthrone as predominating mutagens. ► Qualitative confirmation with Salmonella strains YG1024 and YG1041.
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content type line 23
ISSN:0160-4120
1873-6750
DOI:10.1016/j.envint.2012.01.010