NMR chemical shift assignments of RNA oligonucleotides to expand the RNA chemical shift database

NMR chemical shift assignments of RNA oligonucleotides to expand the RNA chemical shift database

RNAs play myriad functional and regulatory roles in the cell. Despite their significance, three-dimensional structure elucidation of RNA molecules lags significantly behind that of proteins. NMR-based studies are often rate-limited by the assignment of chemical shifts. Automation of the chemical shi...

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Bibliographic Details
Published in:Biomolecular NMR assignments Vol. 15; no. 2; pp. 479 - 490
Main Authors: Liu, Yaping, Kotar, Anita, Hodges, Tracy L., Abdallah, Kyrillos, Taleb, Mallak H., Bitterman, Brayden A., Jaime, Sara, Schaubroeck, Kyle J., Mathew, Ethan, Morgenstern, Nicholas W., Lohmeier, Anthony, Page, Jordan L., Ratanapanichkich, Matt, Arhin, Grace, Johnson, Breanna L., Cherepanov, Stanislav, Moss, Stephen C., Zuniga, Gisselle, Tilson, Nicholas J., Yeoh, Zoe C., Johnson, Bruce A., Keane, Sarah C.
Format: Journal Article
Language:English
Published: Dordrecht Springer Netherlands 01-10-2021
Springer Nature B.V
Subjects:
Automation
Biochemistry
Biological and Medical Physics
Biophysics
NMR
Nuclear magnetic resonance
Nuclear Magnetic Resonance, Biomolecular
Oligonucleotides
Oligonucleotides - chemistry
Physics
Physics and Astronomy
Polymer Sciences
Ribonucleic acid
RNA
RNA - chemistry
Chemical shift database
GAGA tetraloop
RNA
Solution NMR spectroscopy
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Summary:RNAs play myriad functional and regulatory roles in the cell. Despite their significance, three-dimensional structure elucidation of RNA molecules lags significantly behind that of proteins. NMR-based studies are often rate-limited by the assignment of chemical shifts. Automation of the chemical shift assignment process can greatly facilitate structural studies, however, accurate chemical shift predictions rely on a robust and complete chemical shift database for training. We searched the Biological Magnetic Resonance Data Bank (BMRB) to identify sequences that had no (or limited) chemical shift information. Here, we report the chemical shift assignments for 12 RNA hairpins designed specifically to help populate the BMRB.
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ISSN:1874-2718
1874-270X
1874-270X
DOI:10.1007/s12104-021-10049-0