Search Results - "Loechler, Edward L"

Environmental and occupational causes of cancer: new evidence 2005-2007 by Clapp, Richard W, Jacobs, Molly M, Loechler, Edward L

“…What do we currently know about the occupational and environmental causes of cancer? As of 2007, the International Agency for Research on Cancer (IARC)…”
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Architecture of Y-Family DNA Polymerases Relevant to Translesion DNA Synthesis as Revealed in Structural and Molecular Modeling Studies by Chandani, Sushil, Jacobs, Christopher, Loechler, Edward L.

“…DNA adducts, which block replicative DNA polymerases (DNAPs), are often bypassed by lesion-bypass DNAPs, which are mostly in the Y-Family. Y-Family DNAPs can…”
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How Y-Family DNA polymerase IV is more accurate than Dpo4 at dCTP insertion opposite an N2-dG adduct of benzo[a]pyrene by Sholder, Gabriel, Creech, Amanda, Loechler, Edward L.

“…•Investigating how Y-Family DNA polymerases insert dCTP with benzo[a]pyrene-N2-dG.•Four structural elements are key.•Large minor groove opening accommodates…”
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A method to accurately quantitate intensities of (32)P-DNA bands when multiple bands appear in a single lane of a gel is used to study dNTP insertion opposite a benzo[a]pyrene-dG adduct by Sulfolobus DNA polymerases Dpo4 and Dbh by Sholder, Gabriel, Loechler, Edward L

“…Quantitating relative (32)P-band intensity in gels is desired, e.g., to study primer-extension kinetics of DNA polymerases (DNAPs). Following imaging, multiple…”
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A method to accurately quantitate intensities of 32P-DNA bands when multiple bands appear in a single lane of a gel is used to study dNTP insertion opposite a benzo[a]pyrene-dG adduct by Sulfolobus DNA polymerases Dpo4 and Dbh by Sholder, Gabriel, Loechler, Edward L.

“…•A scan-method to accurately quantitate overlapping 32P-bands is described.•The scan-method is superior to current box-methods to quantitate 32P-bands.•dNTP…”
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The Precautionary Principle in Environmental Science by Kriebel, David, Tickner, Joel, Epstein, Paul, Lemons, John, Levins, Richard, Loechler, Edward L., Quinn, Margaret, Rudel, Ruthann, Schettler, Ted, Stoto, Michael

“…Environmental scientists play a key role in society's responses to environmental problems, and many of the studies they perform are intended ultimately to…”
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Structural model of the Y-Family DNA polymerase V/RecA mutasome by Chandani, Sushil, Loechler, Edward L.

“…[Display omitted] ► Y-Family DNA polymerase V models explain most experimental findings. ► Model for DNA polymerase V at a RecA filament prior to UmuD…”
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Genetic effects of oxidative DNA damages: comparative mutagenesis of the imidazole ring-opened formamidopyrimidines (Fapy lesions) and 8-oxo-purines in simian kidney cells by Kalam, M. Abul, Haraguchi, Kazuhiro, Chandani, Sushil, Loechler, Edward L., Moriya, Maasaki, Greenberg, Marc M., Basu, Ashis K.

“…Fapy·dG and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dG) are formed in DNA by hydroxyl radical damage. In order to study replication past these lesions in…”
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Mutagenesis studies of the major benzo[a]pyrene N2-dG adduct in a 5′-TG versus a 5′-UG sequence: removal of the methyl group causes a modest decrease in the [G→T/G→A] mutational ratio by Nagalingam, Arumugam, Seo, Kwang-Young, Loechler, Edward L.

“…The potent mutagen/carcinogen benzo[a]pyrene (B[a]P) is metabolically activated to (+)-anti-B[a]PDE, which induces a full spectrum of mutations primarily at…”
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Mirror image stereoisomers of the major benzo[α]pyrene N2-dG adduct are bypassed by different lesion-bypass DNA polymerases in E. coli by KWANG YOUNG SEO, NAGALINGAM, Arumugam, MIRI, Shadi, JUN YIN, CHANDANI, Sushil, KOLBANOVSKIY, Alexander, SHASTRY, Anant, LOECHLER, Edward L

“…The potent mutagen/carcinogen benzo[a]pyrene (B[a]P) is metabolically activated to (+)-anti-B[a]PDE, which induces a full spectrum of mutations (e.g., G-to-T,…”
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Y-Family DNA polymerases may use two different dNTP shapes for insertion: A hypothesis and its implications by Chandani, Sushil, Loechler, Edward L.

“…Chemicals and radiation can damage DNA leading to the formation of adducts/lesions, which – if not removed by DNA repair pathways – usually block replicative…”
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Amino Acid Architecture That Influences dNTP Insertion Efficiency in Y-Family DNA Polymerase V of E. coli by Seo, Kwang Young, Yin, Jun, Donthamsetti, Prashant, Chandani, Sushil, Lee, Chui Hong, Loechler, Edward L.

“…Y-family DNA polymerases (DNAPs) are often required in cells to synthesize past DNA-containing lesions, such as [+ ta]-B[ a]P- N 2-dG, which is the major…”
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Molecular modeling benzo[a]pyrene N-dG adducts in the two overlapping active sites of the Y-family DNA polymerase Dpo4 by Chandani, Sushil, Loechler, Edward L

“…The potent, ubiquitous environmental mutagen/carcinogen benzo[a]pyrene (B[a]P) induces a single major adduct [+ta]-B[a]P-N2-dG, whose bypass in most cases…”
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Homology modeling of four Y-family, lesion-bypass DNA polymerases: The case that E. coli Pol IV and human Pol κ are orthologs, and E. coli Pol V and human Pol η are orthologs by Lee, Chiu Hong, Chandani, Sushil, Loechler, Edward L.

“…Y-family DNA polymerases (DNAPs) are a superfamily of evolutionarily related proteins that exist in cells to bypass DNA damage caused by both radiation and…”
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DNA polymerase II (polB) is involved in a new DNA repair pathway for DNA interstrand cross-links in Escherichia coli by Berardini, M, Foster, P L, Loechler, E L

“…DNA-DNA interstrand cross-links are the cytotoxic lesions for many chemotherapeutic agents. A plasmid with a single nitrogen mustard (HN2) interstrand…”
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Factors that influence the mutagenic patterns of DNA adducts from chemical carcinogens by Seo, Kwang-Young, Jelinsky, Scott A., Loechler, Edward L.

“…Carcinogens are generally mutagens, which is understandable given that tumor cells grow uncontrollably because they have mutations in critical genes involved…”
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Molecular modeling benzo[a]pyrene N2-dG adducts in the two overlapping active sites of the Y-family DNA polymerase Dpo4 by Chandani, Sushil, Loechler, Edward L

“…The potent, ubiquitous environmental mutagen/carcinogen benzo[a]pyrene (B[a]P) induces a single major adduct [+ta]-B[a]P-N2-dG, whose bypass in most cases…”
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The role of adduct site-specific mutagenesis in understanding how carcinogen-DNA adducts cause mutations: perspective, prospects and problems by Loechler, E L

“…Usually, a particular mutagen/carcinogen forms adducts at many sites in DNA, making it impossible to determine which type of adduct causes which mutation and…”
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Free-Energy Perturbation Methods to Study Structure and Energetics of DNA Adducts:  Results for the Major N2-dG Adduct of Benzo[a]pyrene in Two Conformations and Different Sequence Contexts by Chandani, Sushil, Lee, Chiu Hong, Loechler, Edward L

“…The potent mutagen/carcinogen benzo[a]pyrene (B[a]P) is activated to (+)-anti-B[a]PDE, which induces a variety of mutations (e.g., G → T, G → A, etc.) via its…”
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Mutagenesis studies with four stereoisomeric N2-dG benzo[a]pyrene adducts in the identical 5′-CGC sequence used in NMR studies: G→T mutations dominate in each case by Seo, Kwang-Young, Nagalingam, Arumugam, Tiffany, Matthew, Loechler, Edward L.

“…Benzo[a]pyrene (B[a]P) is a polycyclic aromatic hydrocarbon (PAH) and a potent mutagen/carcinogen found ubiquitously in the environment. B[a]P is primarily…”
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