Search Results - "Lloyd W. Ruddock"

Protein disulfide isomerase: a critical evaluation of its function in disulfide bond formation by Hatahet, Feras, Ruddock, Lloyd W

“…Disulfide bond formation is probably involved in the biogenesis of approximately one third of human proteins. A central player in this essential process is…”
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Design of an alternate antibody fragment format that can be produced in the cytoplasm of Escherichia coli by Tungekar, Aatir A., Ruddock, Lloyd W.

“…With increased accessibility and tissue penetration, smaller antibody formats such as antibody fragments (Fab) and single chain variable fragments (scFv) show…”
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Efficient Production of Fc Fusion Proteins in the Cytoplasm of Escherichia coli : Dissecting and Mitigating Redox Heterogeneity by Tungekar, Aatir A, Ruddock, Lloyd W

“…Cost-effective production of therapeutic proteins in microbial hosts is an indispensable tool towards accessible healthcare. Many of these heterologously…”
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N-glycan processing in ER quality control by Ruddock, Lloyd W, Molinari, Maurizio

“…Glycosylation of asparagine residues in Asn-x-Ser/Thr motifs is a common covalent modification of proteins in the lumen of the endoplasmic reticulum (ER). By…”
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The human protein disulphide isomerase family: substrate interactions and functional properties by Ellgaard, Lars, Ruddock, Lloyd W.

“…The process of disulphide bond formation in the endoplasmic reticulum of eukaryotic cells was one of the first mechanisms of catalysed protein folding to be…”
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Biophysical and structural studies of fibulin-2 by Sohail, Anil A., Koski, M. Kristian, Ruddock, Lloyd W.

“…Fibulin-2 is a multidomain, disulfide-rich, homodimeric protein which belongs to a broader extracellular matrix family. It plays an important role in the…”
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Complementarity determining regions and frameworks contribute to the disulfide bond independent folding of intrinsically stable scFv by Gąciarz, Anna, Ruddock, Lloyd W

“…CyDisCo is a system facilitating disulfide bond formation in recombinant proteins in the cytoplasm of Escherichia coli. Previously we screened for soluble…”
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Production of neutralizing antibody fragment variants in the cytoplasm of E. coli for rapid screening: SARS-CoV-2 a case study by Tungekar, Aatir A., Recacha, Rosario, Ruddock, Lloyd W.

“…Global health challenges such as the coronavirus pandemic warrant the urgent need for a system that allows efficient production of diagnostic and therapeutic…”
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The crystal structure of human microsomal triglyceride transfer protein by Biterova, Ekaterina I., Isupov, Michail N., Keegan, Ronan M., Lebedev, Andrey A., Sohail, Anil A., Liaqat, Inam, Alanen, Heli I., Ruddock, Lloyd W.

“…Microsomal triglyceride transfer protein (MTP) plays an essential role in lipid metabolism, especially in the biogenesis of very low-density lipoproteins and…”
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Structures of Angptl3 and Angptl4, modulators of triglyceride levels and coronary artery disease by Biterova, Ekaterina, Esmaeeli, Mariam, Alanen, Heli I., Saaranen, Mirva, Ruddock, Lloyd W.

“…Coronary artery disease is the most common cause of death globally and is linked to a number of risk factors including serum low density lipoprotein, high…”
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Substrate recognition by the protein disulfide isomerases by Hatahet, Feras, Ruddock, Lloyd W.

“…Protein folding in the endoplasmic reticulum is often associated with the formation of native disulfide bonds. Their primary function is to stabilize the…”
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Structural analysis of human NHLRC2, mutations of which are associated with FINCA disease by Biterova, Ekaterina, Ignatyev, Alexander, Uusimaa, Johanna, Hinttala, Reetta, Ruddock, Lloyd W

“…NHLRC2 (NHL repeat-containing protein 2) is an essential protein. Mutations of NHLRC2, including Asp148Tyr, have been recently associated with a novel FINCA…”
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High-resolution Crystal Structure of Human pERp1, A Saposin-like Protein Involved in IgA, IgM and Integrin Maturation in the Endoplasmic Reticulum by Sowa, Sven T., Moilanen, Antti, Biterova, Ekaterina, Saaranen, Mirva J, Lehtiö, Lari, Ruddock, Lloyd W

“…[Display omitted] •The CNPY family are specialized protein folding factors in the ER.•Here we present the high-resolution crystal structure of human pERp1.•The…”
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Systematic screening of soluble expression of antibody fragments in the cytoplasm of E. coli by Gaciarz, Anna, Veijola, Johanna, Uchida, Yuko, Saaranen, Mirva J, Wang, Chunguang, Hörkkö, Sohvi, Ruddock, Lloyd W

“…Disulfide bonds are the most common structural, post-translational modification found in proteins. Antibodies contain up to 25 disulfide bonds depending on…”
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Efficient soluble expression of disulfide bonded proteins in the cytoplasm of Escherichia coli in fed-batch fermentations on chemically defined minimal media by Gąciarz, Anna, Khatri, Narendar Kumar, Velez-Suberbie, M Lourdes, Saaranen, Mirva J, Uchida, Yuko, Keshavarz-Moore, Eli, Ruddock, Lloyd W

“…The production of recombinant proteins containing disulfide bonds in Escherichia coli is challenging. In most cases the protein of interest needs to be either…”
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Low-molecular-weight oxidants involved in disulfide bond formation by Ruddock, Lloyd W

“…The biogenesis of most secreted and outer membrane proteins involves the formation of structure stabilizing disulfide bonds. Hence knowledge of the mechanisms…”
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Reexamination of the Role of Interplay between Glutathione and Protein Disulfide Isomerase by Lappi, Anna-Kaisa, Ruddock, Lloyd W.

“…Protein disulfide isomerase (PDI) has an essential role in the process of disulfide bond formation, where it catalyzes disulfide bond formation, reduction, and…”
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Division of labor among oxidoreductases: TMX1 preferentially acts on transmembrane polypeptides by Pisoni, Giorgia Brambilla, Ruddock, Lloyd W, Bulleid, Neil, Molinari, Maurizio

“…The endoplasmic reticulum (ER) is the site of maturation for secretory and membrane proteins in eukaryotic cells. The lumen of the mammalian ER contains >20…”
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Production of Extracellular Matrix Proteins in the Cytoplasm of E. coli : Making Giants in Tiny Factories by Sohail, Anil A, Gaikwad, Madhuri, Khadka, Prakash, Saaranen, Mirva J, Ruddock, Lloyd W

“…is the most widely used protein production host in academia and a major host for industrial protein production. However, recombinant production of eukaryotic…”
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Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli by Nguyen, Van Dat, Hatahet, Feras, Salo, Kirsi E H, Enlund, Eveliina, Zhang, Chi, Ruddock, Lloyd W

“…Disulfide bonds are one of the most common post-translational modifications found in proteins. The production of proteins that contain native disulfide bonds…”
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